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Literature summary extracted from

  • Akers, W.J.; Xu, B.; Lee, H.; Sudlow, G.; Fields, G.B.; Achilefu, S.; Edwards, W.B.
    Detection of MMP-2 and MMP-9 activity in vivo with a triple-helical peptide optical probe (2012), Bioconjug. Chem., 23, 656-663.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.24.24 ilomastat a pan-MMP inhibitor, strong inhibition Homo sapiens
3.4.24.35 ilomastat a pan-MMP inhibitor, strong inhibition Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.24.24 additional information
-
 additional information kinetic analysis of LS276-THP hydrolysis by MMP-2 Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.24 Homo sapiens
-
-
-
3.4.24.35 Homo sapiens
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.24.24 fibrosarcoma cell tumor periphery Homo sapiens
-
3.4.24.24 HT-1080 cell tumour cells developed in mice, high expression level Homo sapiens
-
3.4.24.35 fibrosarcoma cell tumor periphery Homo sapiens
-
3.4.24.35 HT-1080 cell tumour cells developed in mice, high expression level Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.24 LS276-THP + H2O development and evaluation of an activatable NIR fluorescent probe LS276-THP for in vivo detection of cancer-related matrix metalloproteinase activity based on a triplehelical peptide substrate with high specificity for MMP-2 and MMP-9 relative to other members of the MMP family, overview. Triple-helical peptides are suitable for highly specific in vivo detection of tumor-related MMP-2 and MMP-9 activity Homo sapiens ?
-
 ?
3.4.24.35 LS276-THP + H2O development and evaluation of an activatable NIR fluorescent probe LS276-THP for in vivo detection of cancer-related matrix metalloproteinase activity based on a triplehelical peptide substrate with high specificity for MMP-2 and MMP-9 relative to other members of the MMP family, overview. Triple-helical peptides are suitable for highly specific in vivo detection of tumor-related MMP-2 and MMP-9 activity Homo sapiens ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.24.24 MMP-2
-
 Homo sapiens
3.4.24.35 MMP-9
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.24.24 37
-
 assay at Homo sapiens
3.4.24.35 37
-
 assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.24.24 7.4
-
 assay at Homo sapiens
3.4.24.35 7.4
-
 assay at Homo sapiens

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
3.4.24.24 0.0000005
-
 pH 7.4, 37°C Homo sapiens ilomastat
3.4.24.35 0.0000002
-
 pH 7.4, 37°C Homo sapiens ilomastat

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.4.24.24 30000
-
 LS276-THP pH 7.4, 37°C Homo sapiens