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Literature summary extracted from

  • Andrade, D.; Assis, D.M.; Santos, J.A.; Alves, F.M.; Hirata, I.Y.; Araujo, M.S.; Blaber, S.I.; Blaber, M.; Juliano, M.A.; Juliano, L.
    Substrate specificity of kallikrein-related peptidase 13 activated by salts or glycosaminoglycans and a search for natural substrate candidates (2011), Biochimie, 93, 1701-1709.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.4.21.119 chondroitin
-
 Homo sapiens
3.4.21.119 dermatan sulfate
-
 Homo sapiens
3.4.21.119 enterokinase
-
 Homo sapiens
3.4.21.119 Sodium citrate
-
 Homo sapiens
3.4.21.119 Sodium sulfate
-
 Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.21.119 expressed in a baculovirus/insect cell line system Homo sapiens

General Stability

EC Number General Stability Organism
3.4.21.119 KLK13 activity is reduced close to zero in the presence of 50 mM Tris HCl (with no sodium citrate or heparin) Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.21.119 0.0012
-
 Abz-KLRSSKQ-EDDnp in the presence of 1.4 M sodium citrate, in 50 mM Tris, 1 mM EDTA, pH 7.5, at 37°C Homo sapiens
3.4.21.119 0.0022
-
 Abz-KLRSSKQ-EDDnp in the presence of 0.0005 mM heparin, in 50 mM Tris, 1 mM EDTA, pH 7.5, at 37°C Homo sapiens
3.4.21.119 0.0053
-
 Abz-KLKSSKQ-EDDnp in the presence of 1.4 M sodium citrate, in 50 mM Tris, 1 mM EDTA, pH 7.5, at 37°C Homo sapiens
3.4.21.119 0.031
-
 Abz-KLKSSKQ-EDDnp in the presence of 0.0005 mM heparin, in 50 mM Tris, 1 mM EDTA, pH 7.5, at 37°C Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.21.119 additional information Na+, K+, Mg2+ and Ca2+ up to 1 M concentration do not activate the enzyme Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.119 Homo sapiens
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.4.21.119 glycoprotein
-
 Homo sapiens

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.21.119 Ni-NTA column chromatography Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.21.119 cervix
-
 Homo sapiens
-
3.4.21.119 esophagus
-
 Homo sapiens
-
3.4.21.119 salivary gland
-
 Homo sapiens
-
3.4.21.119 testis
-
 Homo sapiens
-
3.4.21.119 tonsil
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.119 Abz-GFSPFRSSRIQ-EDDnp + H2O
-
 Homo sapiens Abz-GFSPFR + SSRIQ-EDDnp
-
 ?
3.4.21.119 Abz-KLKSSKQ-EDDnp + H2O
-
 Homo sapiens Abz-KLK + SSKQ-EDDnp
-
 ?
3.4.21.119 Abz-KLRKSKQ-EDDnp + H2O
-
 Homo sapiens Abz-KLR + KSKQ-EDDnp
-
 ?
3.4.21.119 Abz-KLRRSKQ-EDDnp + H2O
-
 Homo sapiens Abz-KLR + RSKQ-EDDnp
-
 ?
3.4.21.119 Abz-KLRSSKQ-EDDnp + H2O best substrate Homo sapiens Abz-KLR + SSKQ-EDDnp
-
 ?
3.4.21.119 Abz-MISLMKRPPGFSPFRSSRI-NH2 + H2O rapid cleavage Homo sapiens Abz-MISLMKRPPGFSPFR + SSRI-NH2
-
 ?
3.4.21.119 Abz-MISLMKRPQ-EDDnp + H2O
-
 Homo sapiens Abz-MISLMK + RPQ-EDDnp
-
 ?
3.4.21.119 histatin 3 + H2O i.e. DSHAKRHHGYKRKFHEKHHSHRGYRSNYLYDN, efficient substrate Homo sapiens DSHAKRHHGYKRKFHEKHHSHRGYR + SNYLYDN
-
 ?
3.4.21.119 low molecular weight human kininogen + H2O
-
 Homo sapiens ?
-
 ?
3.4.21.119 additional information KLK13 does not hydrolyze Abz-KLRESKQ-EDDnp, Abz-KLRSEKQ-EDDnp, and AbzeELRSSKQ-EDDnp Homo sapiens ?
-
 ?
3.4.21.119 additional information the S2' subsite of KLK13 does not have restricted specificity but does present preference for the basic amino acid R and does not accepted negative charge Homo sapiens ?
-
 ?
3.4.21.119 myelin basic protein + H2O at least two cleavage sites are identified at YGGR-ASDYK and PRHR-DTGI Homo sapiens ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.21.119 ? x * about 30000, SDS-PAGE Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
3.4.21.119 kallikrein-related peptidase 13
-
 Homo sapiens
3.4.21.119 KLK13
-
 Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.21.119 0.1
-
 Abz-KLKSSKQ-EDDnp in the presence of 1.4 M sodium citrate, in 50 mM Tris, 1 mM EDTA, pH 7.5, at 37°C Homo sapiens
3.4.21.119 0.5
-
 Abz-KLKSSKQ-EDDnp in the presence of 0.0005 mM heparin, in 50 mM Tris, 1 mM EDTA, pH 7.5, at 37°C Homo sapiens
3.4.21.119 3.2
-
 Abz-KLRSSKQ-EDDnp in the presence of 0.0005 mM heparin, in 50 mM Tris, 1 mM EDTA, pH 7.5, at 37°C Homo sapiens
3.4.21.119 3.4
-
 Abz-KLRSSKQ-EDDnp in the presence of 1.4 M sodium citrate, in 50 mM Tris, 1 mM EDTA, pH 7.5, at 37°C Homo sapiens

General Information

EC Number General Information Comment Organism
3.4.21.119 physiological function KLK13 plays a role in the defense of the upper digestive apparatus and in male reproductive organs Homo sapiens

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.4.21.119 16
-
 Abz-KLKSSKQ-EDDnp in the presence of 0.0005 mM heparin, in 50 mM Tris, 1 mM EDTA, pH 7.5, at 37°C Homo sapiens
3.4.21.119 19
-
 Abz-KLKSSKQ-EDDnp in the presence of 1.4 M sodium citrate, in 50 mM Tris, 1 mM EDTA, pH 7.5, at 37°C Homo sapiens
3.4.21.119 1455
-
 Abz-KLRSSKQ-EDDnp in the presence of 0.0005 mM heparin, in 50 mM Tris, 1 mM EDTA, pH 7.5, at 37°C Homo sapiens
3.4.21.119 2834
-
 Abz-KLRSSKQ-EDDnp in the presence of 1.4 M sodium citrate, in 50 mM Tris, 1 mM EDTA, pH 7.5, at 37°C Homo sapiens