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Literature summary extracted from

  • Mazumder, S.; Ganguly, A.; Ali, N.
    The effect of C-terminal domain deletion on the catalytic activity of Leishmania donovani surface proteinase GP63: Role of Ser446 in proteolysis (2010), Biochimie, 92, 1876-1885.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.24.36 DNA and amino acid sequence determination, analysis and comparison, overexpression in Escherichia coli strain BL21 (DE3) pLysS Leishmania donovani donovani

Protein Variants

EC Number Protein Variants Comment Organism
3.4.24.36 F448V site-directed mutagenesis, the mutant shows activity similar to the wild-type enzyme Leishmania donovani donovani
3.4.24.36 F448Y site-directed mutagenesis, the mutant shows activity similar to the wild-type enzyme Leishmania donovani donovani
3.4.24.36 additional information construction of several truncation mutants, deletion of about 180-211 amino acids, DELTA420 and DELTA389, from the C-terminal region results in approximately 50% reduction in residual activitycompared to the wild-type enzyme Leishmania donovani donovani
3.4.24.36 S446A site-directed mutagenesis, the mutant shows 50% reduced activity and altered kinetics compared to the wild-type enzyme Leishmania donovani donovani
3.4.24.36 S446T site-directed mutagenesis, the mutant shows activity similar to the wild-type enzyme Leishmania donovani donovani

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.24.36 0.0037
-
 casein fluorogenic substrate, pH 7.2, 37°C, wild-type enzyme Leishmania donovani donovani
3.4.24.36 0.005
-
 casein fluorogenic substrate, pH 7.2, 37°C, mutant F448V Leishmania donovani donovani
3.4.24.36 0.0052
-
 casein fluorogenic substrate, pH 7.2, 37°C, mutant F448Y Leishmania donovani donovani
3.4.24.36 0.0053
-
 casein fluorogenic substrate, pH 7.2, 37°C, truncated mutant DELTA542 Leishmania donovani donovani
3.4.24.36 0.0054
-
 casein fluorogenic substrate, pH 7.2, 37°C, mutant S446T Leishmania donovani donovani
3.4.24.36 0.0055
-
 casein fluorogenic substrate, pH 7.2, 37°C, truncated mutant DELTA484 Leishmania donovani donovani
3.4.24.36 0.0098
-
 casein fluorogenic substrate, pH 7.2, 37°C, mutant S446A Leishmania donovani donovani
3.4.24.36 0.0104
-
 casein fluorogenic substrate, pH 7.2, 37°C, truncated mutant DELTA389 Leishmania donovani donovani
3.4.24.36 0.011
-
 casein fluorogenic substrate, pH 7.2, 37°C, truncated mutant DELTA420 Leishmania donovani donovani

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.24.36 Zn2+ zinc proteinase Leishmania donovani donovani

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.36 Leishmania donovani donovani C7EX18
-
-
3.4.24.36 Leishmania donovani donovani MHOM/IN/1983/AG83 C7EX18
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.24.36 recombinant enzyme from Escherichia coli strain BL21 (DE3) pLysS by nickel affinity chromatography and ultrafiltration Leishmania donovani donovani

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.36 azocasein + H2O
-
 Leishmania donovani donovani ?
-
 ?
3.4.24.36 azocasein + H2O
-
 Leishmania donovani donovani MHOM/IN/1983/AG83 ?
-
 ?
3.4.24.36 casein + H2O fluorescein isothiocyanate-labeled casein Leishmania donovani donovani ?
-
 ?
3.4.24.36 casein + H2O fluorescein isothiocyanate-labeled casein Leishmania donovani donovani MHOM/IN/1983/AG83 ?
-
 ?
3.4.24.36 Gelatin + H2O
-
 Leishmania donovani donovani ?
-
 ?
3.4.24.36 Gelatin + H2O
-
 Leishmania donovani donovani MHOM/IN/1983/AG83 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.24.36 More homology modeling and structure-function relationship of GP63, overview Leishmania donovani donovani

Synonyms

EC Number Synonyms Comment Organism
3.4.24.36 gp63
-
 Leishmania donovani donovani
3.4.24.36 surface proteinase GP63
-
 Leishmania donovani donovani

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.24.36 37
-
 assay at Leishmania donovani donovani

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.24.36 3.1
-
 casein fluorogenic substrate, pH 7.2, 37°C, mutant S446A Leishmania donovani donovani
3.4.24.36 3.5
-
 casein fluorogenic substrate, pH 7.2, 37°C, truncated mutant DELTA389 Leishmania donovani donovani
3.4.24.36 3.7
-
 casein fluorogenic substrate, pH 7.2, 37°C, truncated mutant DELTA420 Leishmania donovani donovani
3.4.24.36 11.1
-
 casein fluorogenic substrate, pH 7.2, 37°C, truncated mutant DELTA484 Leishmania donovani donovani
3.4.24.36 11.8
-
 casein fluorogenic substrate, pH 7.2, 37°C, mutant F448Y Leishmania donovani donovani
3.4.24.36 11.8
-
 casein fluorogenic substrate, pH 7.2, 37°C, truncated mutant DELTA542 Leishmania donovani donovani
3.4.24.36 11.9
-
 casein fluorogenic substrate, pH 7.2, 37°C, mutant S446T Leishmania donovani donovani
3.4.24.36 12
-
 casein fluorogenic substrate, pH 7.2, 37°C, wild-type enzyme Leishmania donovani donovani
3.4.24.36 12.7
-
 casein fluorogenic substrate, pH 7.2, 37°C, mutant F448V Leishmania donovani donovani

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.24.36 7.2 7.5 assay at Leishmania donovani donovani

General Information

EC Number General Information Comment Organism
3.4.24.36 evolution GP63 belongs to the metzincin class, encoded by a series of tandemly linked genes usually in a single chromosome that are highly sequence-conserved among different species of Leishmania Leishmania donovani donovani
3.4.24.36 additional information homology modeling and structure-function relationship of GP63, overview Leishmania donovani donovani

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.4.24.36 300
-
 casein fluorogenic substrate, pH 7.2, 37°C, mutant S446A Leishmania donovani donovani
3.4.24.36 300
-
 casein fluorogenic substrate, pH 7.2, 37°C, truncated mutant DELTA389 Leishmania donovani donovani
3.4.24.36 300
-
 casein fluorogenic substrate, pH 7.2, 37°C, truncated mutant DELTA420 Leishmania donovani donovani
3.4.24.36 2000
-
 casein fluorogenic substrate, pH 7.2, 37°C, truncated mutant DELTA484 Leishmania donovani donovani
3.4.24.36 2200
-
 casein fluorogenic substrate, pH 7.2, 37°C, mutant F448Y Leishmania donovani donovani
3.4.24.36 2200
-
 casein fluorogenic substrate, pH 7.2, 37°C, mutant S446T Leishmania donovani donovani
3.4.24.36 2200
-
 casein fluorogenic substrate, pH 7.2, 37°C, truncated mutant DELTA542 Leishmania donovani donovani
3.4.24.36 2500
-
 casein fluorogenic substrate, pH 7.2, 37°C, mutant F448V Leishmania donovani donovani
3.4.24.36 3200
-
 casein fluorogenic substrate, pH 7.2, 37°C, wild-type enzyme Leishmania donovani donovani