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Literature summary extracted from

  • Nosaka, K.
    High affinity of acid phosphatase encoded by PHO3 gene in Saccharomyces cerevisiae for thiamin phosphates (1990), Biochim. Biophys. Acta, 1037, 147-154.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.2 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide highly increases the Km for thiamin phosphate Saccharomyces cerevisiae
3.1.3.2 phosphate competitive inhibition of phosphate-repressible acid phosphatase. iinhibition 4-nitrophenyl phosphate hydrolysis and slight inhibition thiamin phosphate hydrolysis by thiamin-repressible acid phosphatase Saccharomyces cerevisiae
3.1.3.2 thiamin competitive versus thiamin phosphate, slight inhibition 4-nitrophenyl phosphate hydrolysis. 1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide modification of the enzyme causes complete loss of thiamin binding activity. The thiamin binding of the thiamin-repressible acid phosphatase is optimal at pH 5.2 and lost at pH 3.0 Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.2 0.0016
-
 thiamine phosphate thiamine-repressible acid phosphatase, pH 5.0, 37°C Saccharomyces cerevisiae
3.1.3.2 0.0017
-
 thiamine diphosphate thiamine-repressible acid phosphatase, pH 5.0, 37°C Saccharomyces cerevisiae
3.1.3.2 0.0028
-
 thiamine diphosphate thiamine-repressible acid phosphatase, pH 3.0, 37°C Saccharomyces cerevisiae
3.1.3.2 0.0063
-
 thiamine phosphate thiamine-repressible acid phosphatase, pH 3.0, 37°C Saccharomyces cerevisiae
3.1.3.2 0.2
-
 thiamine diphosphate phosphate-repressible acid phosphatase, pH 4.5, 37°C Saccharomyces cerevisiae
3.1.3.2 0.22
-
 4-nitrophenyl phosphate phosphate-repressible acid phosphatase, pH 4.5, 37°C Saccharomyces cerevisiae
3.1.3.2 0.25
-
 4-nitrophenyl phosphate thiamine-repressible acid phosphatase, pH 3.0, 37°C Saccharomyces cerevisiae
3.1.3.2 0.27
-
 thiamine phosphate phosphate-repressible acid phosphatase, pH 4.5, 37°C Saccharomyces cerevisiae
3.1.3.2 1.5
-
 4-nitrophenyl phosphate thiamine-repressible acid phosphatase, pH 5.0, 37°C Saccharomyces cerevisiae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.3.2 periplasmic space thiamin-repressible acid phosphatase Saccharomyces cerevisiae 42597
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.2 60000
-
 x * 60000, about, thiamine-repressible acid phosphatase, sequence calculation Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.3.2 thiamine diphosphate + 2 H2O Saccharomyces cerevisiae
-
 thiamine + 2 phosphate
-
 ?
3.1.3.2 thiamine phosphate + H2O Saccharomyces cerevisiae
-
 thiamine + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.2 Saccharomyces cerevisiae P24031 gene PH03 encoding thiamin-repressible acid phosphatase, gene PH05 encoding phosphate-repressible acid phosphatase
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.1.3.2 glycoprotein the enzyme is highly glycosylated Saccharomyces cerevisiae

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.2 native thiamine-repressible acid phosphatase by cold osmotic shock treatment, anion exchange chromatography, and ultrafiltration Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.2 8.7
-
 purified thiamine-repressible acid phosphatase, substrate glucose 1-phosphate, pH 3.0, 37°C Saccharomyces cerevisiae
3.1.3.2 17.1
-
 purified thiamine-repressible acid phosphatase, substrate thiazole phosphate, pH 3.0, 37°C Saccharomyces cerevisiae
3.1.3.2 61.3
-
 purified thiamine-repressible acid phosphatase, substrate thiamine phosphate, pH 3.0, 37°C Saccharomyces cerevisiae
3.1.3.2 66.2
-
 purified thiamine-repressible acid phosphatase, substrate ATP, pH 3.0, 37°C Saccharomyces cerevisiae
3.1.3.2 71.5
-
 purified thiamine-repressible acid phosphatase, substrate 4-nitrophenyl phosphate, pH 3.0, 37°C Saccharomyces cerevisiae
3.1.3.2 221.2
-
 purified thiamine-repressible acid phosphatase, substrate thiamine diphosphate, pH 3.0, 37°C Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.2 4-nitrophenyl phosphate + H2O
-
 Saccharomyces cerevisiae 4-nitrophenol + phosphate
-
 ?
3.1.3.2 5-hydroxyethyl-4-methylthiazole phosphate + H2O
-
 Saccharomyces cerevisiae 5-hydroxyethyl-4-methylthiazole + phosphate
-
 ?
3.1.3.2 ATP + H2O
-
 Saccharomyces cerevisiae ADP + phosphate
-
 ?
3.1.3.2 D-glucose 1-phosphate + H2O low activity Saccharomyces cerevisiae D-glucose + phosphate
-
 ?
3.1.3.2 additional information the thiamin-repressible acid phosphatase shows no activity with CMP. The phosphate-repressible acid phosphatase shows a broad substrate specificity on phosphate esters Saccharomyces cerevisiae ?
-
 ?
3.1.3.2 thiamine diphosphate + 2 H2O
-
 Saccharomyces cerevisiae thiamine + 2 phosphate
-
 ?
3.1.3.2 thiamine phosphate + H2O
-
 Saccharomyces cerevisiae thiamine + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.2 ? x * 60000, about, thiamine-repressible acid phosphatase, sequence calculation Saccharomyces cerevisiae

Synonyms

EC Number Synonyms Comment Organism
3.1.3.2 orthophosphoric-monoester phosphohydrolase
-
 Saccharomyces cerevisiae
3.1.3.2 phosphate-repressible acid phosphatase
-
 Saccharomyces cerevisiae
3.1.3.2 Pi-rAPase
-
 Saccharomyces cerevisiae
3.1.3.2 T-rAPase
-
 Saccharomyces cerevisiae
3.1.3.2 thiamin-repressible acid phosphatase
-
 Saccharomyces cerevisiae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.3.2 37
-
 assay at Saccharomyces cerevisiae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.2 3
-
 thiamine-repressible acid phosphatase Saccharomyces cerevisiae
3.1.3.2 4.5
-
 assay at, phosphate-repressible acid phosphatase Saccharomyces cerevisiae

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.3.2 3 5 activity range, thiamine-repressible acid phosphatase Saccharomyces cerevisiae

Expression

EC Number Organism Comment Expression
3.1.3.2 Saccharomyces cerevisiae the thiamin-repressible acid phosphatase is repressed by thiamine, but not by phosphate down