Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Sevrioukova, I.F.; Poulos, T.L.
    Arginines 65 and 310 in putidaredoxin reductase are critical for interaction with putidaredoxin (2010), Biochemistry, 49, 5160-5166.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.18.1.5 crystal structure of a covalently linked putidaredoxin reductase (Pdr)-putidaredoxin (Pdx) complex. Residues R65 and R310 are the key elements required for the formation of a productive electron transfer complex with Pdx. The C-terminal lysine cluster assists in Pdx docking by fine-tuning Pdr-Pdx interactions to achieve the optimal geometry between the redox centers, and the basic surface residues in Pdr-like ferredoxin reductases not only define specificity for the redox partner but also may facilitate its dissociation Pseudomonas putida

Protein Variants

EC Number Protein Variants Comment Organism
1.18.1.5 K339A moderate decrease in the binding affinity and reduction of Pdx Pseudomonas putida
1.18.1.5 K387A moderate decrease in the binding affinity and reduction of Pdx Pseudomonas putida
1.18.1.5 K409A moderate decrease in the binding affinity and reduction of Pdx Pseudomonas putida
1.18.1.5 N384A mutation has no effect on the Pdr-Pdx interaction Pseudomonas putida
1.18.1.5 R310A mutation lowers the interprotein electron tranfer rate by 20-30fold without perturbing the Pdx association step Pseudomonas putida
1.18.1.5 R310E mutation decreases both the Pdr-to-Pdx ET and partner binding affinity by 100- and 8fold, respectively Pseudomonas putida
1.18.1.5 R65A mutation lowers the interprotein electron tranfer rate by 20-30fold without perturbing the Pdx association step Pseudomonas putida

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.18.1.5 0.017
-
 NADH mutant R65A, pH 7.5, 25°C, cosubstrate dichlorophenolindophenol Pseudomonas putida
1.18.1.5 0.018
-
 NADH mutant R310A, pH 7.5, 25°C, cosubstrate dichlorophenolindophenol Pseudomonas putida
1.18.1.5 0.022
-
 NADH mutant R339A, pH 7.5, 25°C, cosubstrate dichlorophenolindophenol Pseudomonas putida
1.18.1.5 0.025
-
 NADH mutant R339A, pH 7.5, 25°C, cosubstrate K3Fe(CN)6 Pseudomonas putida
1.18.1.5 0.027
-
 NADH wild-type, pH 7.5, 25°C, cosubstrate dichlorophenolindophenol Pseudomonas putida
1.18.1.5 0.029
-
 NADH mutant K409A, pH 7.5, 25°C, cosubstrate K3Fe(CN)6 Pseudomonas putida
1.18.1.5 0.029
-
 oxidized 2,6-dichlorophenolindolphenol mutant R65A, pH 7.5, 25°C Pseudomonas putida
1.18.1.5 0.031
-
 NADH mutant K409A, pH 7.5, 25°C, cosubstrate dichlorophenolindophenol Pseudomonas putida
1.18.1.5 0.034
-
 NADH mutant R65A, pH 7.5, 25°C, cosubstrate K3Fe(CN)6 Pseudomonas putida
1.18.1.5 0.036
-
 NADH mutant K387A, pH 7.5, 25°C, cosubstrate K3Fe(CN)6 Pseudomonas putida
1.18.1.5 0.036
-
 Fe(CN)63- mutant K409A, pH 7.5, 25°C Pseudomonas putida
1.18.1.5 0.036
-
 NADH mutant R310A, pH 7.5, 25°C, cosubstrate K3Fe(CN)6 Pseudomonas putida
1.18.1.5 0.037
-
 NADH mutant R310E, pH 7.5, 25°C, cosubstrate dichlorophenolindophenol Pseudomonas putida
1.18.1.5 0.037
-
 NADH mutant R310E, pH 7.5, 25°C, cosubstrate K3Fe(CN)6 Pseudomonas putida
1.18.1.5 0.038
-
 NADH wild-type, pH 7.5, 25°C, cosubstrate K3Fe(CN)6 Pseudomonas putida
1.18.1.5 0.04
-
 Fe(CN)63- wild-type, pH 7.5, 25°C Pseudomonas putida
1.18.1.5 0.04
-
 Fe(CN)63- mutant R310E, pH 7.5, 25°C Pseudomonas putida
1.18.1.5 0.042
-
 NADH mutant K387A, pH 7.5, 25°C, cosubstrate dichlorophenolindophenol Pseudomonas putida
1.18.1.5 0.043
-
 Fe(CN)63- mutant N384A, pH 7.5, 25°C Pseudomonas putida
1.18.1.5 0.044
-
 Fe(CN)63- mutant K387A, pH 7.5, 25°C Pseudomonas putida
1.18.1.5 0.045
-
 Fe(CN)63- mutant R339A, pH 7.5, 25°C Pseudomonas putida
1.18.1.5 0.05
-
 NADH mutant N384A, pH 7.5, 25°C, cosubstrate dichlorophenolindophenol Pseudomonas putida
1.18.1.5 0.05
-
 NADH mutant N384A, pH 7.5, 25°C, cosubstrate K3Fe(CN)6 Pseudomonas putida
1.18.1.5 0.05
-
 Fe(CN)63- mutant R65A, pH 7.5, 25°C Pseudomonas putida
1.18.1.5 0.083
-
 oxidized 2,6-dichlorophenolindolphenol mutant K387A, pH 7.5, 25°C Pseudomonas putida
1.18.1.5 0.083
-
 oxidized 2,6-dichlorophenolindolphenol mutant N384A, pH 7.5, 25°C Pseudomonas putida
1.18.1.5 0.091
-
 oxidized 2,6-dichlorophenolindolphenol mutant K409A, pH 7.5, 25°C Pseudomonas putida
1.18.1.5 0.097
-
 oxidized 2,6-dichlorophenolindolphenol wild-type, pH 7.5, 25°C Pseudomonas putida
1.18.1.5 0.125
-
 oxidized 2,6-dichlorophenolindolphenol mutant R310A, pH 7.5, 25°C Pseudomonas putida
1.18.1.5 0.125
-
 oxidized 2,6-dichlorophenolindolphenol mutant R339A, pH 7.5, 25°C Pseudomonas putida
1.18.1.5 0.143
-
 Fe(CN)63- mutant R310A, pH 7.5, 25°C Pseudomonas putida
1.18.1.5 0.2
-
 oxidized 2,6-dichlorophenolindolphenol mutant R310E, pH 7.5, 25°C Pseudomonas putida

Organism

EC Number Organism UniProt Comment Textmining
1.18.1.5 Pseudomonas putida P16640
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.18.1.5 2 ferricytochrome c + NADH
-
 Pseudomonas putida 2 ferrocytochrome c + NAD+ + H+
-
 ?
1.18.1.5 NADH + H+ + Fe(CN)63-
-
 Pseudomonas putida NAD+ + Fe(CN)62-
-
 ?
1.18.1.5 NADH + H+ + oxidized 2,6-dichlorophenolindophenol
-
 Pseudomonas putida NAD+ + reduced 2,6-dichlorophenolindophenol
-
 ?
1.18.1.5 reduced putidaredoxin + NAD+
-
 Pseudomonas putida oxidized putidaredoxin + NADH + H+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.18.1.5 Pdr
-
 Pseudomonas putida

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.18.1.5 203
-
 oxidized 2,6-dichlorophenolindolphenol mutant R65A, pH 7.5, 25°C Pseudomonas putida
1.18.1.5 283
-
 oxidized 2,6-dichlorophenolindolphenol mutant R310E, pH 7.5, 25°C Pseudomonas putida
1.18.1.5 288
-
 oxidized 2,6-dichlorophenolindolphenol mutant R339A, pH 7.5, 25°C Pseudomonas putida
1.18.1.5 358
-
 oxidized 2,6-dichlorophenolindolphenol mutant N384A, pH 7.5, 25°C Pseudomonas putida
1.18.1.5 377
-
 oxidized 2,6-dichlorophenolindolphenol mutant K409A, pH 7.5, 25°C Pseudomonas putida
1.18.1.5 385
-
 oxidized 2,6-dichlorophenolindolphenol wild-type, pH 7.5, 25°C Pseudomonas putida
1.18.1.5 408
-
 oxidized 2,6-dichlorophenolindolphenol mutant K387A, pH 7.5, 25°C Pseudomonas putida
1.18.1.5 543
-
 Fe(CN)63- mutant R339A, pH 7.5, 25°C Pseudomonas putida
1.18.1.5 560
-
 Fe(CN)63- mutant R65A, pH 7.5, 25°C Pseudomonas putida
1.18.1.5 617
-
 oxidized 2,6-dichlorophenolindolphenol mutant R310A, pH 7.5, 25°C Pseudomonas putida
1.18.1.5 650
-
 NADH mutant R339A, pH 7.5, 4°C Pseudomonas putida
1.18.1.5 720
-
 NADH mutant R310E, pH 7.5, 4°C Pseudomonas putida
1.18.1.5 729
-
 NADH mutant R310A, pH 7.5, 4°C Pseudomonas putida
1.18.1.5 754
-
 NADH mutant R65A, pH 7.5, 4°C Pseudomonas putida
1.18.1.5 755
-
 Fe(CN)63- mutant K409A, pH 7.5, 25°C Pseudomonas putida
1.18.1.5 772
-
 Fe(CN)63- mutant K387A, pH 7.5, 25°C Pseudomonas putida
1.18.1.5 780
-
 NADH mutant K387A, pH 7.5, 4°C Pseudomonas putida
1.18.1.5 790
-
 Fe(CN)63- mutant R310E, pH 7.5, 25°C Pseudomonas putida
1.18.1.5 800
-
 NADH mutant K409A, pH 7.5, 4°C Pseudomonas putida
1.18.1.5 806
-
 NADH wild-type, pH 7.5, 4°C Pseudomonas putida
1.18.1.5 820
-
 NADH mutant N384A, pH 7.5, 4°C Pseudomonas putida
1.18.1.5 898
-
 Fe(CN)63- mutant N384A, pH 7.5, 25°C Pseudomonas putida
1.18.1.5 982
-
 Fe(CN)63- wild-type, pH 7.5, 25°C Pseudomonas putida
1.18.1.5 1485
-
 Fe(CN)63- mutant R310A, pH 7.5, 25°C Pseudomonas putida