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Literature summary extracted from

  • Chen, M.; Poulter, C.D.
    Characterization of thermophilic archaeal isopentenyl phosphate kinases. (2010), Biochemistry, 49, 207-217.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.4.26 0.0027
-
 isopentenyl diphosphate pH 7.5, 37°C Thermoplasma acidophilum
2.7.4.26 0.0031
-
 ADP pH 7.5, 37°C Thermoplasma acidophilum
2.7.4.26 0.0044
-
 isopentenyl phosphate pH 7.5, 37°C Thermoplasma acidophilum
2.7.4.26 0.006
-
 ATP pH 7.5, 37°C Thermoplasma acidophilum
2.7.4.26 0.0076
-
 isopentenyl diphosphate pH 7.5, 37°C Methanothermobacter thermautotrophicus
2.7.4.26 0.0127
-
 isopentenyl phosphate pH 7.5, 37°C Methanothermobacter thermautotrophicus
2.7.4.26 0.013
-
 ADP pH 7.5, 37°C Methanothermobacter thermautotrophicus
2.7.4.26 0.0134
-
 ATP pH 7.5, 37°C Methanothermobacter thermautotrophicus
2.7.4.26 0.0145
-
 isopentenyl thiolophosphate pH 7.5, 37°C Thermoplasma acidophilum
2.7.4.26 0.0237
-
 isopentenyl thiolophosphate pH 7.5, 37°C Methanothermobacter thermautotrophicus
2.7.4.26 0.0263
-
 butyl phosphate pH 7.5, 37°C Methanothermobacter thermautotrophicus
2.7.4.26 0.0297
-
 but-3-en-1-yl phosphate pH 7.5, 37°C Methanothermobacter thermautotrophicus
2.7.4.26 0.0432
-
 dimethylallyl phosphate pH 7.5, 37°C Methanothermobacter thermautotrophicus
2.7.4.26 0.103
-
 but-3-en-1-yl phosphate pH 7.5, 37°C Thermoplasma acidophilum
2.7.4.26 0.173
-
 butyl phosphate pH 7.5, 37°C Thermoplasma acidophilum
2.7.4.26 0.174
-
 dimethylallyl phosphate pH 7.5, 37°C Thermoplasma acidophilum
2.7.4.26 0.74
-
 geranyl phosphate pH 7.5, 37°C Methanothermobacter thermautotrophicus
2.7.4.26 4.7
-
 geranyl phosphate pH 7.5, 37°C Thermoplasma acidophilum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.4.26 ATP + isopentenyl phosphate Thermoplasma acidophilum
-
 ADP + isopentenyl diphosphate
-
 ?
2.7.4.26 ATP + isopentenyl phosphate Methanothermobacter thermautotrophicus
-
 ADP + isopentenyl diphosphate
-
 ?
2.7.4.26 ATP + isopentenyl phosphate Methanothermobacter thermautotrophicus Delta H
-
 ADP + isopentenyl diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.4.26 Methanothermobacter thermautotrophicus O26153
-
-
2.7.4.26 Methanothermobacter thermautotrophicus Delta H O26153
-
-
2.7.4.26 Thermoplasma acidophilum Q9HLX1
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.4.26 ATP + but-3-en-1-yl phosphate low reaction rate Thermoplasma acidophilum ADP + but-3-en-1-yl diphosphate
-
 ?
2.7.4.26 ATP + but-3-en-1-yl phosphate low reaction rate Methanothermobacter thermautotrophicus ADP + but-3-en-1-yl diphosphate
-
 ?
2.7.4.26 ATP + but-3-en-1-yl phosphate low reaction rate Methanothermobacter thermautotrophicus Delta H ADP + but-3-en-1-yl diphosphate
-
 ?
2.7.4.26 ATP + butyl phosphate low reaction rate Thermoplasma acidophilum ADP + butyl diphosphate
-
 ?
2.7.4.26 ATP + butyl phosphate low reaction rate Methanothermobacter thermautotrophicus ADP + butyl diphosphate
-
 ?
2.7.4.26 ATP + butyl phosphate low reaction rate Methanothermobacter thermautotrophicus Delta H ADP + butyl diphosphate
-
 ?
2.7.4.26 ATP + dimethylallyl phosphate low reaction rate Thermoplasma acidophilum ADP + dimethylallyl diphosphate
-
 ?
2.7.4.26 ATP + dimethylallyl phosphate low reaction rate Methanothermobacter thermautotrophicus ADP + dimethylallyl diphosphate
-
 ?
2.7.4.26 ATP + dimethylallyl phosphate low reaction rate Methanothermobacter thermautotrophicus Delta H ADP + dimethylallyl diphosphate
-
 ?
2.7.4.26 ATP + geranyl phosphate low reaction rate Thermoplasma acidophilum ADP + geranyl diphosphate
-
 ?
2.7.4.26 ATP + geranyl phosphate low reaction rate Methanothermobacter thermautotrophicus ADP + geranyl diphosphate
-
 ?
2.7.4.26 ATP + isopentenyl phosphate
-
 Thermoplasma acidophilum ADP + isopentenyl diphosphate
-
 ?
2.7.4.26 ATP + isopentenyl phosphate
-
 Thermoplasma acidophilum ADP + isopentenyl diphosphate
-
 r
2.7.4.26 ATP + isopentenyl phosphate
-
 Methanothermobacter thermautotrophicus ADP + isopentenyl diphosphate
-
 ?
2.7.4.26 ATP + isopentenyl phosphate
-
 Methanothermobacter thermautotrophicus ADP + isopentenyl diphosphate
-
 r
2.7.4.26 ATP + isopentenyl phosphate
-
 Methanothermobacter thermautotrophicus Delta H ADP + isopentenyl diphosphate
-
 ?
2.7.4.26 ATP + isopentenyl phosphate
-
 Methanothermobacter thermautotrophicus Delta H ADP + isopentenyl diphosphate
-
 r
2.7.4.26 ATP + isopentenyl thiolophosphate low reaction rate Thermoplasma acidophilum ADP + isopentenyl thiolodiphosphate
-
 ?
2.7.4.26 ATP + isopentenyl thiolophosphate low reaction rate Methanothermobacter thermautotrophicus ADP + isopentenyl thiolodiphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.4.26 homodimer
-
 Thermoplasma acidophilum
2.7.4.26 homodimer
-
 Methanothermobacter thermautotrophicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.4.26 70
-
-
 Thermoplasma acidophilum
2.7.4.26 70
-
-
 Methanothermobacter thermautotrophicus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.7.4.26 55 75
-
 Thermoplasma acidophilum
2.7.4.26 55 75
-
 Methanothermobacter thermautotrophicus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.4.26 50
-
 stable up to Methanothermobacter thermautotrophicus
2.7.4.26 70
-
 stable up to Thermoplasma acidophilum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.4.26 0.0142
-
 geranyl phosphate pH 7.5, 37°C Methanothermobacter thermautotrophicus
2.7.4.26 0.047
-
 geranyl phosphate pH 7.5, 37°C Thermoplasma acidophilum
2.7.4.26 2.75
-
 isopentenyl diphosphate pH 7.5, 37°C, reverse reaction Thermoplasma acidophilum
2.7.4.26 2.84
-
 isopentenyl thiolophosphate pH 7.5, 37°C Thermoplasma acidophilum
2.7.4.26 4.5
-
 isopentenyl diphosphate pH 7.5, 37°C, reverse reaction Methanothermobacter thermautotrophicus
2.7.4.26 8
-
 isopentenyl phosphate pH 7.5, 37°C Thermoplasma acidophilum
2.7.4.26 8.07
-
 but-3-en-1-yl phosphate pH 7.5, 37°C Thermoplasma acidophilum
2.7.4.26 8.22
-
 isopentenyl thiolophosphate pH 7.5, 37°C Methanothermobacter thermautotrophicus
2.7.4.26 9.9
-
 butyl phosphate pH 7.5, 37°C Thermoplasma acidophilum
2.7.4.26 11.3
-
 dimethylallyl phosphate pH 7.5, 37°C Thermoplasma acidophilum
2.7.4.26 20.5
-
 but-3-en-1-yl phosphate pH 7.5, 37°C Methanothermobacter thermautotrophicus
2.7.4.26 27.5
-
 isopentenyl phosphate pH 7.5, 37°C Methanothermobacter thermautotrophicus
2.7.4.26 31.8
-
 butyl phosphate pH 7.5, 37°C Methanothermobacter thermautotrophicus
2.7.4.26 34
-
 dimethylallyl phosphate pH 7.5, 37°C Methanothermobacter thermautotrophicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.4.26 7.7
-
-
 Thermoplasma acidophilum
2.7.4.26 8.5
-
 broad optimum Methanothermobacter thermautotrophicus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.4.26 6.5 8.4 less than 50% of maximal activity above and below Thermoplasma acidophilum
2.7.4.26 7 9 less than 50% of maximal activity above and below Methanothermobacter thermautotrophicus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.7.4.26 0.01
-
 geranyl phosphate pH 7.5, 37°C Thermoplasma acidophilum
2.7.4.26 0.19
-
 geranyl phosphate pH 7.5, 37°C Methanothermobacter thermautotrophicus
2.7.4.26 5.7
-
 butyl phosphate pH 7.5, 37°C Thermoplasma acidophilum
2.7.4.26 6.5
-
 dimethylallyl phosphate pH 7.5, 37°C Thermoplasma acidophilum
2.7.4.26 7.8
-
 but-3-en-1-yl phosphate pH 7.5, 37°C Thermoplasma acidophilum
2.7.4.26 69
-
 but-3-en-1-yl phosphate pH 7.5, 37°C Methanothermobacter thermautotrophicus
2.7.4.26 120
-
 butyl phosphate pH 7.5, 37°C Methanothermobacter thermautotrophicus
2.7.4.26 200
-
 isopentenyl thiolophosphate pH 7.5, 37°C Thermoplasma acidophilum
2.7.4.26 340
-
 ADP pH 7.5, 37°C Methanothermobacter thermautotrophicus
2.7.4.26 350
-
 isopentenyl thiolophosphate pH 7.5, 37°C Methanothermobacter thermautotrophicus
2.7.4.26 590
-
 isopentenyl phosphate pH 7.5, 37°C Methanothermobacter thermautotrophicus
2.7.4.26 790
-
 dimethylallyl phosphate pH 7.5, 37°C Methanothermobacter thermautotrophicus
2.7.4.26 1000
-
 isopentenyl phosphate pH 7.5, 37°C Thermoplasma acidophilum
2.7.4.26 1200
-
 ADP pH 7.5, 37°C Thermoplasma acidophilum
2.7.4.26 1300
-
 ATP pH 7.5, 37°C Thermoplasma acidophilum
2.7.4.26 1800
-
 isopentenyl phosphate pH 7.5, 37°C Thermoplasma acidophilum
2.7.4.26 2200
-
 ATP pH 7.5, 37°C Methanothermobacter thermautotrophicus
2.7.4.26 2200
-
 isopentenyl phosphate pH 7.5, 37°C Methanothermobacter thermautotrophicus