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Literature summary extracted from

  • McCarty, R.M.; Somogyi, A.; Lin, G.; Jacobsen, N.E.; Bandarian, V.
    The deazapurine biosynthetic pathway revealed: in vitro enzymatic synthesis of PreQ(0) from guanosine 5-triphosphate in four steps (2009), Biochemistry, 48, 3847-3852.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.3.99.3 gene queE, expression of His-tagged QueE in Escherichia coli strain BL21(DE3) from plasmid pRM78 Bacillus subtilis
6.3.4.20 cloning of His-tagged QueC and expression in Escherichia coli strain BL21(DE3) Bacillus subtilis

Protein Variants

EC Number Protein Variants Comment Organism
6.3.4.20 additional information in vitro preparation of the deazapurine nucleoside, preQ0, by the successive action of the four involved enzymes, i.e. Escherichia coli QueD, Bacillus subtilis QueE and QueC, and Streptomyces rimosus ToyM, cloning and expression in Escherichia coli strain BL21(DE3), overview Bacillus subtilis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.3.99.3 6-carboxy-5,6,7,8-tetrahydropterin Bacillus subtilis
-
 7-carboxy-7-deazaguanine + NH3
-
 ?
6.3.4.20 7-carboxy-7-carbaguanine + NH3 + ATP Bacillus subtilis
-
 7-cyano-7-carbaguanine + ADP + phosphate + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.3.99.3 Bacillus subtilis
-
 gene queE
-
6.3.4.20 Bacillus subtilis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.3.99.3 recombinant His-tagged QueE from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Bacillus subtilis
6.3.4.20 recombinant His-tagged QueC from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration Bacillus subtilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.3.99.3 6-carboxy-5,6,7,8-tetrahydropterin
-
 Bacillus subtilis 7-carboxy-7-deazaguanine + NH3
-
 ?
4.3.99.3 6-carboxy-5,6,7,8-tetrahydropterin substrate from a coupled reaction with Escherichia coli CPH4 synthase Bacillus subtilis 7-carboxy-7-deazaguanine + NH3
-
 ?
6.3.4.20 7-carboxy-7-carbaguanine + NH3 + ATP
-
 Bacillus subtilis 7-cyano-7-carbaguanine + ADP + phosphate + H2O
-
 ?
6.3.4.20 7-carboxy-7-carbaguanine + NH3 + ATP dependent conversion of a carboxylic acid to a nitrile Bacillus subtilis 7-cyano-7-carbaguanine + ADP + phosphate + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.3.99.3 queE
-
 Bacillus subtilis
6.3.4.20 queC
-
 Bacillus subtilis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.3.99.3 7.4
-
 assay at Bacillus subtilis
6.3.4.20 7.4
-
 assay at Bacillus subtilis

Cofactor

EC Number Cofactor Comment Organism Structure
4.3.99.3 S-adenosyl-L-methionine dependent on. Members of the radical SAM superfamily contain a CX3CX2C-motif for binding a [4Fe-4S] cluster, which when reductively activated, cleaves SAM to 5?-deoxyadenosyl radical, which in turn initiates radical mediated transformations of the substrate Bacillus subtilis
6.3.4.20 ATP
-
 Bacillus subtilis

General Information

EC Number General Information Comment Organism
4.3.99.3 evolution QueE is a member of the radical SAM organic radical generating enzyme superfamily. Members of the radical SAM superfamily contain a CX3CX2C-motif for binding a [4Fe-4S] cluster, which when reductively activated, cleaves SAM to 5'-deoxyadenosyl radical, which in turn initiates radical mediated transformations of the substrate Bacillus subtilis
4.3.99.3 metabolism QueE is involved in the queuosine biosynthetic pathway of Bacillus subtilis Bacillus subtilis
6.3.4.20 metabolism the metabolic pathway includes the conversion of the biosynthetic intermediate, 6-carboxy-5,6,7,8-tetrahydropterin, to intermediate, 7-carboxy-7-deazaguanine, CDG, by an unusual transformation catalyzed by QueE, a member of the radical SAM enzyme superfamily. The carboxylate moiety on CDG is converted subsequently to a nitrile to yield preQ0 by QueC in an ATP-dependent reaction, in which ammonia serves as the nitrogen source. CDG may be the central precursor to all deazapurines, biosynthesis of deazapurine containing compounds in nature incorporates H2NTP, CPH4 and CDG as common intermediates Bacillus subtilis