EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
2.1.1.253 | Ti(III) citrate | dependent on | Methanosarcina barkeri |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.1.1.252 | Mg2+ | - |
Methanosarcina barkeri | |
2.1.1.253 | Mg2+ | - |
Methanosarcina barkeri |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.1.1.252 | 50000 | - |
x * 50000, SDS-PAGE | Methanosarcina barkeri |
2.1.1.253 | 40000 | - |
x * 40000, SDS-PAGE | Methanosarcina barkeri |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.1.1.247 | Methanosarcina barkeri | - |
gene mtbA | - |
2.1.1.247 | Methanosarcina barkeri NaT1 | - |
gene mtbA | - |
2.1.1.252 | Methanosarcina barkeri | - |
gene mtqB | - |
2.1.1.252 | Methanosarcina barkeri NaT1 | - |
gene mtqB | - |
2.1.1.253 | Methanosarcina barkeri | - |
gene mtqA | - |
2.1.1.253 | Methanosarcina barkeri NaT1 | - |
gene mtqA | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.1.1.252 | native enzyme by ultracentrifugation, a first step of anion exchange chromatography, hydroxyapatite chromatography, a second step of anion exchange chromatography, adsorption and again a third step of anion exchange chromatography | Methanosarcina barkeri |
2.1.1.253 | native enzyme by ultracentrifugation, a first step of anion exchange chromatography, hydroxyapatite chromatography, a second step of anion exchange chromatography, adsorption and again a third step of anion exchange chromatography | Methanosarcina barkeri |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
2.1.1.247 | culture condition:trimethylamine-grown cell | - |
Methanosarcina barkeri | - |
2.1.1.247 | additional information | cell extracts of strain NaT1 grown on trimethylamine rather than on tetramethylammonium are devoid of tetramethylammonium:coenzyme M methyltransferase activity | Methanosarcina barkeri | - |
2.1.1.253 | culture condition:tetramethylammonium-grown cell | - |
Methanosarcina barkeri | - |
2.1.1.253 | additional information | cell extracts of strain NaT1 grown on trimethylamine rather than on tetramethylammonium are devoid of tetramethylammonium:coenzyme M methyltransferase activity | Methanosarcina barkeri | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.1.1.247 | 0.2 | - |
trimethylamine:coenzyme M methyltransferase activity, pH 7.0, 37°C | Methanosarcina barkeri |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.1.1.247 | additional information | the enzyme exhibits MT2 activity, the MT2 activity is measured by following the coenzyme-M-dependent demethylation of methylcobalamin | Methanosarcina barkeri | ? | - |
? | |
2.1.1.247 | additional information | the enzyme exhibits MT2 activity, the MT2 activity is measured by following the coenzyme-M-dependent demethylation of methylcobalamin | Methanosarcina barkeri NaT1 | ? | - |
? | |
2.1.1.247 | [methyl-Co(III) trimethylamine-specific corrinoid protein] + coenzyme M | - |
Methanosarcina barkeri | methyl-CoM + [Co(I) trimethylamine-specific corrinoid protein] | - |
? | |
2.1.1.247 | [methyl-Co(III) trimethylamine-specific corrinoid protein] + coenzyme M | - |
Methanosarcina barkeri NaT1 | methyl-CoM + [Co(I) trimethylamine-specific corrinoid protein] | - |
? | |
2.1.1.252 | tetramethylammonium + a [Co(I) tetramethylammonium-specific corrinoid protein] | - |
Methanosarcina barkeri | a [methyl-Co(III) tetramethylammonium-specific corrinoid protein] + trimethylamine | - |
? | |
2.1.1.252 | tetramethylammonium + a [Co(I) tetramethylammonium-specific corrinoid protein] | - |
Methanosarcina barkeri NaT1 | a [methyl-Co(III) tetramethylammonium-specific corrinoid protein] + trimethylamine | - |
? | |
2.1.1.253 | additional information | the enzyme exhibits MT2 activity, the MT2 activity is measured by following the coenzyme-M-dependent demethylation of methylcobalamin. Tetramethylammonium:coenzyme M methyltransferase activity detected by coenzyme M methylation | Methanosarcina barkeri | ? | - |
? | |
2.1.1.253 | additional information | the enzyme exhibits MT2 activity, the MT2 activity is measured by following the coenzyme-M-dependent demethylation of methylcobalamin. Tetramethylammonium:coenzyme M methyltransferase activity detected by coenzyme M methylation | Methanosarcina barkeri NaT1 | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.1.1.252 | ? | x * 50000, SDS-PAGE | Methanosarcina barkeri |
2.1.1.253 | ? | x * 40000, SDS-PAGE | Methanosarcina barkeri |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.1.1.247 | mtbA | - |
Methanosarcina barkeri |
2.1.1.247 | trimethylamine:coenzyme M methyltransferase | - |
Methanosarcina barkeri |
2.1.1.252 | MtqB | - |
Methanosarcina barkeri |
2.1.1.253 | MtqA | - |
Methanosarcina barkeri |
2.1.1.253 | tetramethylammonium:coenzyme M methyltransferase | - |
Methanosarcina barkeri |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.1.1.247 | 37 | - |
assay at | Methanosarcina barkeri |
2.1.1.253 | 37 | - |
assay at | Methanosarcina barkeri |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.1.1.247 | 7 | - |
assay at | Methanosarcina barkeri |
2.1.1.253 | 7 | - |
assay at | Methanosarcina barkeri |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
2.1.1.253 | ATP | dependent on | Methanosarcina barkeri |
EC Number | General Information | Comment | Organism |
---|---|---|---|
2.1.1.247 | additional information | cell extracts of strain NaT1 catalyze the formation of methyl-coenzyme M from coenzyme M and tetramethylammonium, EC 2.1.1.253, or trimethylamine, EC 2.1.1.250, but not from coenzyme M and dimethylamine, EC 2.1.1.249, monomethylamine, EC 2.1.1.248, or methanol, EC 2.1.1.246 | Methanosarcina barkeri |
2.1.1.252 | additional information | cell extracts of strain NaT1 catalyze the formation of methyl-coenzyme M from coenzyme M and tetramethylammonium, EC 2.1.1.253, or trimethylamine, EC 2.1.1.250, but not from coenzyme M and dimethylamine, EC 2.1.1.249, monomethylamine, EC 2.1.1.248, or methanol, EC 2.1.1.246 | Methanosarcina barkeri |
2.1.1.253 | additional information | cell extracts of strain NaT1 catalyze the formation of methyl-coenzyme M from coenzyme M and tetramethylammonium, EC 2.1.1.253, or trimethylamine, EC 2.1.1.250, but not from coenzyme M and dimethylamine, EC 2.1.1.249, monomethylamine, EC 2.1.1.248, or methanol, EC 2.1.1.246 | Methanosarcina barkeri |