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Literature summary extracted from

  • Kim, Y.M.; Shimizu, R.; Nakai, H.; Mori, H.; Okuyama, M.; Kang, M.S.; Fujimoto, Z.; Funane, K.; Kim, D.; Kimura, A.
    Truncation of N- and C-terminal regions of Streptococcus mutans dextranase enhances catalytic activity (2011), Appl. Microbiol. Biotechnol., 91, 329-339.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.11 DNA and amino acid sequence determination and analysis, sequence comparisons, expression of His-tagged enzyme in Escherichia coli strain BL21 (DE3) CodonPlus-RIL Streptococcus mutans

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.11 additional information generation of five truncated SmDexs by deleting the N-terminal variable region, the glucan-binding site, or the C-terminal variable region. Two truncation-mutant enzymes devoid of C-terminal variable region or of C-terminal variable region and N-terminal variable region are catalytically active, thereby indicating that the two regions are not essential for the catalytic activity, mutant structures compared to the wild-type enzyme, overview Streptococcus mutans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.11 89800
-
 x * 94537, sequence calculation, x * 95400, unprocessed recombinant enzyme, SDS-PAGE, x * 89800, processed recombinant enzyme, SDS-PAGE Streptococcus mutans
3.2.1.11 94537
-
 x * 94537, sequence calculation, x * 95400, unprocessed recombinant enzyme, SDS-PAGE, x * 89800, processed recombinant enzyme, SDS-PAGE Streptococcus mutans
3.2.1.11 95400
-
 x * 94537, sequence calculation, x * 95400, unprocessed recombinant enzyme, SDS-PAGE, x * 89800, processed recombinant enzyme, SDS-PAGE Streptococcus mutans

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.11 Streptococcus mutans F5BA50
-
-
3.2.1.11 Streptococcus mutans ATCC 25175 F5BA50
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.11 recombinant His-tagged enzyme from Escherichia coli strain BL21 (DE3) CodonPlus-RIL by nickel affinity chromatography, ultrafilatration, and hydrophobic interaction and anion exchange chromatography Streptococcus mutans

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.11 additional information cells are cultivated in brain-heart infusion medium Streptococcus mutans
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.11 3.02
-
 purified recombinant enzyme SmDex95, the 95 kDa variant, pH 5.0, temperature not specified in the publication Streptococcus mutans
3.2.1.11 8.21
-
 purified recombinant enzyme SmDex90, the 90 kDa variant, pH 5.0, temperature not specified in the publication Streptococcus mutans

Storage Stability

EC Number Storage Stability Organism
3.2.1.11 4°C, 2 mM NaN3, 6 months, the purified SmDex90 is proteolytically degraded to more than seven polypeptides of 23-70 kDa during long storage Streptococcus mutans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.11 dextran + H2O
-
 Streptococcus mutans ?
-
 ?
3.2.1.11 dextran + H2O
-
 Streptococcus mutans ATCC 25175 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.11 ? x * 94537, sequence calculation, x * 95400, unprocessed recombinant enzyme, SDS-PAGE, x * 89800, processed recombinant enzyme, SDS-PAGE Streptococcus mutans
3.2.1.11 More the enzyme comprises four regions from the N- to C-termini: N-terminal variable region, N-VR, conserved region, CR, glucan-binding site, GBS, and C-terminal variable region, C-VR Streptococcus mutans

Synonyms

EC Number Synonyms Comment Organism
3.2.1.11 endo-dextranase
-
 Streptococcus mutans
3.2.1.11 More the enzyme belongs to the glycoside hydrolase family (GH) 66 Streptococcus mutans
3.2.1.11 SmDex
-
 Streptococcus mutans

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.11 40
-
 purified recombinant enzymes, stable up to Streptococcus mutans

General Information

EC Number General Information Comment Organism
3.2.1.11 additional information N-terminal variable region and C-terminal variable region of the enzyme are not essentially required for catalytic activity, but induce hindered substrate binding to the active site Streptococcus mutans