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Literature summary extracted from

  • Chang, L.; Ding, M.; Bao, L.; Chen, Y.; Zhou, J.; Lu, H.
    Characterization of a bifunctional xylanase/endoglucanase from yak rumen microorganisms (2011), Appl. Microbiol. Biotechnol., 90, 1933-1942.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.8 Ca2+ activates uncultured microorganism
3.2.1.8 Co2+ activates uncultured microorganism

Application

EC Number Application Comment Organism
3.2.1.8 biofuel production RuCelA can produce xylo-oligosaccharides and cell-oligosaccharides in the continuous saccharification of pretreated rice straw, which can be further degraded into fermentable sugars. Therefor, the bifunctional RuCelA distinguishes itself as an ideal candidate for industrial application uncultured microorganism
3.2.1.73 biofuel production RuCelA can produce xylo-oligosaccharides and cell-oligosaccharides in the continuous saccharification of pretreated rice straw, which can be further degraded into fermentable sugars. Therefor, the bifunctional RuCelA distinguishes itself as an ideal candidate for industrial application uncultured microorganism

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.8 expressed in Escherichia coli BL21/pET21a uncultured microorganism
3.2.1.8 RuCelA, DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain BL21(DE3) uncultured microorganism
3.2.1.73 expressed in Escherichia coli BL21/pET21a uncultured microorganism

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.8 Ca2+ 10 mM, activity decreased to 45% uncultured microorganism
3.2.1.8 Co2+ 10 mM, activity decreased to 60% uncultured microorganism
3.2.1.8 Cu2+ 10 mM, activity decreased to 20% uncultured microorganism
3.2.1.8 Mg2+ 10 mM, activity decreased to 70% uncultured microorganism
3.2.1.8 Zn2+ 10 mM, activity decreased to 15% uncultured microorganism

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.8 additional information
-
 additional information Michaelis-Menten kinetics uncultured microorganism
3.2.1.8 additional information
-
 xylan 0.58 g/l at 65°C, pH 7.0 uncultured microorganism
3.2.1.73 additional information
-
 carboxymethylcellulose Km-value: 2.71 g/l at 50°C, pH5.0 uncultured microorganism

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.73 Co2+ 10 mM, activity increased to 154% uncultured microorganism
3.2.1.73 Cu2+ 10 mM, activity decreased to 86% uncultured microorganism
3.2.1.73 Mg2+ 10 mM, activity decreased to 85% uncultured microorganism

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.8 60600
-
 calculated mass uncultured microorganism
3.2.1.73 60600
-
 calculated mass uncultured microorganism

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.8 additional information uncultured microorganism RuCelA is a bifunctional xylanase/endoglucanase showing activity against xylan and carboxymethyl cellulose. Using xylan and barley glucan as substrates, RuCelA displays obvious synergistic effects with beta-1,4-xylosidase and beta-1,4-glucosidase. RuCelA can produce xylo-oligosaccharides and cello-oligosaccharides in the continuous saccharification of pretreated rice straw ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.8 uncultured microorganism E2DQY5 from Bos mutus rumen
-
3.2.1.8 uncultured microorganism E2DQY5 RuCelA, a bifunctional xylanase/endoglucanase, EC 3.2.1.8 and EC 3.2.1.73; isolated from rumen from a yak rumen metagenomic library, chinese yak, Bos grunniens
-
3.2.1.73 uncultured microorganism E2DQY5 RuCelA, a bifunctional xylanase/endoglucanase, EC 3.2.1.8 and EC 3.2.1.73; isolated from rumen from a yak rumen metagenomic library, chinese yak, Bos grunniens
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.8 the cloned protein from the recombinant strain BL21/pET21a-RuCelA is purified using Ni-NTA resin uncultured microorganism
3.2.1.73 the cloned protein from the recombinant strain BL21/pET21a-RuCelA is purified using Ni-NTA resin uncultured microorganism

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.8 additional information isolated from rumen from a yak rumen metagenomic library, chinese yak, Bos grunniens uncultured microorganism
-
3.2.1.73 additional information isolated from rumen from a yak rumen metagenomic library, chinese yak, Bos grunniens uncultured microorganism
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.8 264.1
-
 purified recombinant enzyme, pH 7.0, 65°C uncultured microorganism
3.2.1.8 264.1
-
 xylan as substrate, pH 7.0, 65°C uncultured microorganism
3.2.1.8 805
-
 lichenin as substrate, pH 7.0, 65°C uncultured microorganism
3.2.1.8 938
-
 barley glucan as substrate, pH 7.0, 65°C uncultured microorganism
3.2.1.73 54.3
-
 carboxymethylcellulose as substrate, pH 5.0, 50°C uncultured microorganism
3.2.1.73 58.6
-
 hydroxyethylcellulose as substrate, pH 5.0, 50°C uncultured microorganism

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.8 barley glucan + H2O
-
 uncultured microorganism ? the main end-products are monosaccharides, disaccharides, trisacchraides, and tetrasaccharides ?
3.2.1.8 birch wood xylan + H2O
-
 uncultured microorganism ?
-
 ?
3.2.1.8 lichenin + H2O
-
 uncultured microorganism ?
-
 ?
3.2.1.8 additional information RuCelA is a bifunctional xylanase/endoglucanase showing activity against xylan and carboxymethyl cellulose. Using xylan and barley glucan as substrates, RuCelA displays obvious synergistic effects with beta-1,4-xylosidase and beta-1,4-glucosidase. RuCelA can produce xylo-oligosaccharides and cello-oligosaccharides in the continuous saccharification of pretreated rice straw uncultured microorganism ?
-
 ?
3.2.1.8 additional information no activitiy with beta-1,3-glucan, laminarin, or Avicel as substrates uncultured microorganism ?
-
 ?
3.2.1.8 xylan + H2O
-
 uncultured microorganism ? the main end-products are monosaccharides, disaccharides, trisacchraides, and tetrasaccharides, mainly xylose, xylobiose, and xylotetrose ?
3.2.1.73 carboxymethylcellulose + H2O
-
 uncultured microorganism ?
-
 ?
3.2.1.73 cellulose + H2O filter paper uncultured microorganism ? main products are cellobiose and cellotriose ?
3.2.1.73 hydroxyethylcellulose + H2O
-
 uncultured microorganism ?
-
 ?
3.2.1.73 additional information no activitiy using beta-1,3-glucan, laminarin, or Avicel as substrates uncultured microorganism ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.8 ? x * 60600, about, sequence calculation uncultured microorganism

Synonyms

EC Number Synonyms Comment Organism
3.2.1.8 bifunctional xylanase/endoglucanase RuCelA, a bifunctional xylanase/endoglucanase, EC 3.2.1.8 and EC 3.2.1.73 uncultured microorganism
3.2.1.8 RuCelA
-
 uncultured microorganism
3.2.1.8 xylanase
-
 uncultured microorganism
3.2.1.73 bifunctional xylanase/endoglucanase RuCelA, a bifunctional xylanase/endoglucanase, EC 3.2.1.8 and EC 3.2.1.73 uncultured microorganism
3.2.1.73 endoglucanase
-
 uncultured microorganism
3.2.1.73 RuCelA
-
 uncultured microorganism

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.8 65
-
-
 uncultured microorganism
3.2.1.73 50
-
-
 uncultured microorganism

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.8 35 75 activity range uncultured microorganism

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.8 30
-
 xylan as substrate, remains stable after treatment for 1 h uncultured microorganism
3.2.1.8 30 50 purified recombinant RuCelA, 1 h, stable uncultured microorganism
3.2.1.8 40
-
 xylan as substrate, remains stable after treatment for 1 h uncultured microorganism
3.2.1.8 50
-
 xylan as substrate, remains stable after treatment for 1 h uncultured microorganism
3.2.1.8 60
-
 purified recombinant RuCelA, 1 h, 60% activity remaining uncultured microorganism
3.2.1.8 60
-
 xylan as substrate, remains 60% activity after treatment for 1 h uncultured microorganism
3.2.1.8 70
-
 purified recombinant RuCelA, 1 h, almost no activity remaining uncultured microorganism
3.2.1.8 70
-
 xylan as substrate, almost loses its activity after treatment for 1 h uncultured microorganism

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.8 7
-
-
 uncultured microorganism
3.2.1.73 5
-
-
 uncultured microorganism

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.8 4 10 activity range uncultured microorganism

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.8 3
-
 purified recombinant RuCelA, 24 h, 55% activity remaining uncultured microorganism
3.2.1.8 3
-
 xylan as substrate, loses 45% of its activity after incubation for 24 h uncultured microorganism
3.2.1.8 4 9 purified recombinant RuCelA, 24 h, 80% activity remaining uncultured microorganism
3.2.1.8 4 9 xylan as substrate, remains more than 80% of its activity uncultured microorganism