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Literature summary extracted from

  • Barabote, R.D.; Parales, J.V.; Guo, Y.Y.; Labavitch, J.M.; Parales, R.E.; Berry, A.M.
    Xyn10A, a thermostable endoxylanase from Acidothermus cellulolyticus 11B (2010), Appl. Environ. Microbiol., 76, 7363-7366.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.8 recombinant expression in Escherichia coli Acidothermus cellulolyticus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.8 0.35
-
 oat spelt xylan pH 6.0, 90°C Acidothermus cellulolyticus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.8 Acidothermus cellulolyticus
-
-
-
3.2.1.8 Acidothermus cellulolyticus 11B
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.8 recombinant enzyme from Escherichia coli by heat treatment at 65°C for 15 min and hydroxyapatite chromatography Acidothermus cellulolyticus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.8 birch wood xylan + H2O best substrate, thin-layer chromatography determination of reaction products, the major products from oat spelt and birchwood xylan have retention factor values between those of xylose and xylopentaose Acidothermus cellulolyticus ?
-
 ?
3.2.1.8 birch wood xylan + H2O best substrate, thin-layer chromatography determination of reaction products, the major products from oat spelt and birchwood xylan have retention factor values between those of xylose and xylopentaose Acidothermus cellulolyticus 11B ?
-
 ?
3.2.1.8 additional information Xyn10A is an endoxylanase. No activity with cellulose, carboxymethyl cellulose, and xanthan gum Acidothermus cellulolyticus ?
-
 ?
3.2.1.8 additional information Xyn10A is an endoxylanase. No activity with cellulose, carboxymethyl cellulose, and xanthan gum Acidothermus cellulolyticus 11B ?
-
 ?
3.2.1.8 oat spelt xylan + H2O thin-layer chromatography determination of reaction products, the major products from oat spelt and birchwood xylan have retention factor values between those of xylose and xylopentaose Acidothermus cellulolyticus ?
-
 ?
3.2.1.8 oat spelt xylan + H2O thin-layer chromatography determination of reaction products, the major products from oat spelt and birchwood xylan have retention factor values between those of xylose and xylopentaose Acidothermus cellulolyticus 11B ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.8 More the enzyme is a family 10 xylanase Acidothermus cellulolyticus
3.2.1.8 Xyn10A
-
 Acidothermus cellulolyticus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.8 90
-
-
 Acidothermus cellulolyticus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.8 60 100 activity range, pH-dependent: at 100°C activity occurs at pH 4.0-6.0, at 60°C activity occurs at pH 5.0-8.0, overview Acidothermus cellulolyticus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.8 90
-
 half-life is 1.5 h in presence of xylan Acidothermus cellulolyticus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.8 6
-
-
 Acidothermus cellulolyticus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.8 4 8 activity range, at pH 8.0 activity occurs only up to 70°C, at pH 7.0 up to 90°C, at pH 4.0 activity occurs at 90-100°C, overview Acidothermus cellulolyticus