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Literature summary extracted from

  • Geng, Y.; Wang, S.; Qi, Q.
    Expression of active recombinant human tissue-type plasminogen activator by using in vivo polyhydroxybutyrate granule display (2010), Appl. Environ. Microbiol., 76, 7226-7230.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.21.68 a truncated version of tPA is fused to the N terminus of the phasin protein with a thrombin cleavage site as the linker, and then expressed in Escherichia coli strain XL1-Blue on the surface of polyhydroxybutyrate granules using phasin as the affinity tag. Untreate enzyme is expressed in inactive inclusion bodies in the bacteria. The in vivo surface display strategy for functional rPA expression in Escherichia coli is distinct for its efficient folding and easier purification, method optimization, overview. Subcloning in Escherichia coli strain DH5alpha Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.21.68 plasminogen + H2O Homo sapiens
-
 plasmin + ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.68 Homo sapiens
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.68 plasminogen + H2O
-
 Homo sapiens plasmin + ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.21.68 Tissue plasminogen activator
-
 Homo sapiens
3.4.21.68 tPA
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.21.68 37
-
 assay at Homo sapiens