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Literature summary extracted from

  • Pavezzi, F.C.; Carneiro, A.A.; Bocchini-Martins, D.A.; Alves-Prado, H.F.; Ferreira, H.; Martins, P.M.; Gomes, E.; da Silva, R.
    Influence of different substrates on the production of a mutant thermostable glucoamylase in submerged fermentation (2011), Appl. Biochem. Biotechnol., 163, 14-24.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.3 expression of wild-type and mutant S54P/T314A/H415Y enzymes in Saccharomyces cerevisiae Aspergillus awamori

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.3 S54P/T314A/H415Y the mutant enzyme is more thermostable compared to the wild-type enzyme at 70°C. The mutation does not affect the protein secretion nor the production of the enzyme Aspergillus awamori

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.3 100000
-
 mutant S54P/T314A/H415Y Aspergillus awamori

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.3 Aspergillus awamori
-
-
-
3.2.1.3 Aspergillus awamori C468
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.3 recombinant enzyme mutant S54P/T314A/H415Y from Saccharomyces cerevisiae by acarbose affinity chromatography Aspergillus awamori

Synonyms

EC Number Synonyms Comment Organism
3.2.1.3 glucoamylase
-
 Aspergillus awamori

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.3 65
-
 mutant S54P/T314A/H415Y Aspergillus awamori

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.3 60
-
 purified recombinant enzyme mutant S54P/T314A/H415Y, stable up to, irreversible thermo-inactivation of the mutant enzyme follows first-order kinetics Aspergillus awamori
3.2.1.3 65
-
 purified recombinant enzyme mutant S54P/T314A/H415Y, half-life is 76 min Aspergillus awamori

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.3 4.5
-
 mutant S54P/T314A/H415Y Aspergillus awamori

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.3 5.5 9 the recombinant enzyme mutant S54P/T314A/H415Y retains 80% of its activity within this range Aspergillus awamori