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Literature summary extracted from

  • Dashper, S.G.; Pan, Y.; Veith, P.D.; Chen, Y.Y.; Toh, E.C.; Liu, S.W.; Cross, K.J.; Reynolds, E.C.
    Lactoferrin inhibits Porphyromonas gingivalis proteinases and has sustained biofilm inhibitory activity (2012), Antimicrob. Agents Chemother., 56, 1548-1556.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.22.37 lactoferrin inhibits both the Arg- and Lys-specific proteinase activities of Porphyromonas gingivalis whole cells by approximately 40% at 1 mg/ml and over 70% at 10 mg/ml. Lactoferrin inhibits both the Arg-specific and Lys-specific activities of purified Porphyromonas gingivalis 248 RgpA/Kgp proteinase-adhesin complexes by 96% at a concentration of 5 mg/mL; lactoferrin is incubated with purified RgpB which lacks the adhesin domains of RgA and Kgp. Lactoferrin inhibits RgpB activity by 77% at a concentration of 1.0 mg/ml and by 95% at 10 mg/ml confirming the inhibition is independent of adhesins Porphyromonas gingivalis
3.4.22.47 lactoferrin inhibits both the Arg- and Lys-specific proteinase activities of Porphyromonas gingivalis whole cells by approximately 40% at 1 mg/ml and over 70% at 10 mg/ml. Lactoferrin inhibits both the Arg-specific and Lys-specific activities of purified Porphyromonas gingivalis 248 RgpA/Kgp proteinase-adhesin complexes by 96% at a concentration of 5 mg/mL Porphyromonas gingivalis

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.37 Porphyromonas gingivalis
-
-
-
3.4.22.47 Porphyromonas gingivalis
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.37 Nalpha-benzoyl-L-Arg-4-nitroanilide + H2O
-
 Porphyromonas gingivalis Nalpha-benzoyl-L-Arg + 4-nitroaniline
-
 ?
3.4.22.47 N-(p-tosyl)-Gly-Pro-Lys 4-nitroanilide + H2O
-
 Porphyromonas gingivalis ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.22.37 RgpA
-
 Porphyromonas gingivalis
3.4.22.37 RgpB
-
 Porphyromonas gingivalis
3.4.22.47 KGP
-
 Porphyromonas gingivalis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.22.37 37
-
 assay at Porphyromonas gingivalis
3.4.22.47 37
-
 assay at Porphyromonas gingivalis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.22.37 8
-
 assay at Porphyromonas gingivalis
3.4.22.47 8
-
 assay at Porphyromonas gingivalis

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.4.22.37 0.005
-
 lactoferrin pH 8.0, 37°C, a kinetic analysis of the inhibition of Arg-specific proteolytic activity of purified gingivalis RgpA/Kgp proteinase-adhesin complexes by lactoferrin demonstrates time-dependent inhibition with a first-order inactivation rate constant (kinact) of 0.023/min Porphyromonas gingivalis