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Literature summary extracted from

  • Chapla, D.; Patel, H.; Singh, A.; Madamwar, D.; Shah, A.
    Production, purification and properties of a cellulase-free thermostable endoxylanase from newly isolated Paenibacillus sp. ASCD2 (2012), Ann. Microbiol., 62, 825-834.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.8 Cu2+
-
 Paenibacillus sp.
3.2.1.8 EDTA
-
 Paenibacillus sp.
3.2.1.8 Hg2+
-
 Paenibacillus sp.
3.2.1.8 Mn2+
-
 Paenibacillus sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.8 K+ activates Paenibacillus sp.
3.2.1.8 Mg2+ activates Paenibacillus sp.
3.2.1.8 additional information the enzyme activity is unaffected by Fe2+, Zn2+, and Tween 80 Paenibacillus sp.
3.2.1.8 Na+ activates Paenibacillus sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.8 20200
-
 x * 20200, SDS-PAGE Paenibacillus sp.

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.8 Paenibacillus sp.
-
-
-
3.2.1.8 Paenibacillus sp. ASCD2
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.8 native enzyme 16.6fold by ammonium sulfate fractionation and gel filtration Paenibacillus sp.

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.8 additional information optimization of xylanase production under solid state fermentation, maximal activity on wheat straw with organic nitrogen rich medium, pH 7.0, at a moisture ratio of 1:5, at 50°C Paenibacillus sp.
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.8 87
-
 purified native enzyme, pH 7.0, 60°C, substrate birchwood xylan Paenibacillus sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.8 birchwood xylan + H2O the endoxylanase produces only xylooligosaccharides from birchwood xylan Paenibacillus sp. xylooligosaccharides
-
 ?
3.2.1.8 birchwood xylan + H2O the endoxylanase produces only xylooligosaccharides from birchwood xylan Paenibacillus sp. ASCD2 xylooligosaccharides
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.8 ? x * 20200, SDS-PAGE Paenibacillus sp.

Synonyms

EC Number Synonyms Comment Organism
3.2.1.8 endoxylanase
-
 Paenibacillus sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.8 60
-
-
 Paenibacillus sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.8 65
-
 purified native enzyme, half-life is 415.12 min Paenibacillus sp.
3.2.1.8 70
-
 purified native enzyme, half-life is 102.8 min Paenibacillus sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.8 7
-
-
 Paenibacillus sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.8 6.5 7.5 70% of maximal activity at pH 6.5, 85% at pH 7.5 Paenibacillus sp.