BRENDA - Enzyme Database

Identification and characterization of the cofactor-independent phosphoglycerate mutases of Dirofilaria immitis and its Wolbachia endosymbiont

Li, Z.; Galvin, B.D.; Raverdy, S.; Carlow, C.K.; Vet. Parasitol. 176, 350-356 (2011)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
5.4.2.12
additional information
independent on 2,3-bisphosphoglycerate
Dirofilaria immitis
5.4.2.12
additional information
independent on 2,3-bisphosphoglycerate
Wolbachia sp.
Cloned(Commentary)
EC Number
Commentary
Organism
5.4.2.1
expressed in Escherichia coli C2566/T7 cells
Dirofilaria immitis
5.4.2.1
expressed in Escherichia coli C2566/T7 cells
Wolbachia endosymbiont of Dirofilaria immitis
5.4.2.12
expressed in Escherichia coli C2566/T7 cells
Wolbachia endosymbiont of Dirofilaria immitis
5.4.2.12
expressed in Escherichia coli C2566/T7 cells; expression of C-terminally His6-tagged enzyme in Escherichia coli strain C2566/T7
Dirofilaria immitis
5.4.2.12
expression of C-terminally His-tagged enzyme in Escherichia coli strain C2566/T7
Wolbachia sp.
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
5.4.2.1
0.55
-
3-phospho-D-glycerate
in 30 mM Tris-HCl, 5 mM MgSO4, 20 mM KCl, pH 7.5, at 30°C
Wolbachia endosymbiont of Dirofilaria immitis
5.4.2.1
0.58
-
3-phospho-D-glycerate
in 30 mM Tris-HCl, 5 mM MgSO4, 20 mM KCl, pH 7.5, at 30°C
Dirofilaria immitis
5.4.2.12
additional information
-
additional information
Michaelis-Menten kinetics
Dirofilaria immitis
5.4.2.12
additional information
-
additional information
Michaelis-Menten kinetics
Wolbachia sp.
5.4.2.12
0.55
-
3-phospho-D-glycerate
in 30 mM Tris-HCl, 5 mM MgSO4, 20 mM KCl, pH 7.5, at 30°C
Wolbachia endosymbiont of Dirofilaria immitis
5.4.2.12
0.55
-
3-phospho-D-glycerate
recombinant His-tagged enzyme, pH 7.5, 30°C
Wolbachia sp.
5.4.2.12
0.58
-
3-phospho-D-glycerate
in 30 mM Tris-HCl, 5 mM MgSO4, 20 mM KCl, pH 7.5, at 30°C; recombinant His-tagged enzyme, pH 7.5, 30°C
Dirofilaria immitis
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
5.4.2.1
56000
-
x * 56000, calculated from amino acid sequence
Wolbachia endosymbiont of Dirofilaria immitis
5.4.2.1
57000
-
x * 57000, calculated from amino acid sequence
Dirofilaria immitis
5.4.2.12
56000
-
x * 56000, calculated from amino acid sequence
Wolbachia endosymbiont of Dirofilaria immitis
5.4.2.12
57000
-
x * 57000, calculated from amino acid sequence
Dirofilaria immitis
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
5.4.2.12
2-phospho-D-glycerate
Dirofilaria immitis
-
3-phospho-D-glycerate
-
-
r
5.4.2.12
2-phospho-D-glycerate
Wolbachia sp.
-
3-phospho-D-glycerate
-
-
r
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
5.4.2.1
Dirofilaria immitis
F4ZE01
-
-
5.4.2.1
Wolbachia endosymbiont of Dirofilaria immitis
F4ZE02
-
-
5.4.2.12
Dirofilaria immitis
-
-
-
5.4.2.12
Dirofilaria immitis
F4ZE01
-
-
5.4.2.12
Wolbachia endosymbiont of Dirofilaria immitis
F4ZE02
-
-
5.4.2.12
Wolbachia sp.
-
endosymbiont of filarial parasite Brugia malayi
-
Purification (Commentary)
EC Number
Commentary
Organism
5.4.2.1
HiTrap nickel chelating column chromatography and HiTrap Q column chromatography
Dirofilaria immitis
5.4.2.1
HiTrap nickel chelating column chromatography and HiTrap Q column chromatography
Wolbachia endosymbiont of Dirofilaria immitis
5.4.2.12
HiTrap nickel chelating column chromatography and HiTrap Q column chromatography
Wolbachia endosymbiont of Dirofilaria immitis
5.4.2.12
HiTrap nickel chelating column chromatography and HiTrap Q column chromatography; recombinant His6-tagged enzyme from Escherichia coli strain C2566/T7 by nickel affinity chromatography, dialysis and anion exchange chromatography
Dirofilaria immitis
5.4.2.12
recombinant His6-tagged enzyme from Escherichia coli strain C2566/T7 by nicke affinity chromatography, dialysis and anion exchange chromatography
Wolbachia sp.
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
5.4.2.12
additional information
optimal enzyme production at 37°C
Wolbachia sp.
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
5.4.2.1
71
-
in 30 mM Tris-HCl, 5 mM MgSO4, 20 mM KCl, pH 7.5, at 30°C
Wolbachia endosymbiont of Dirofilaria immitis
5.4.2.1
225
-
in 30 mM Tris-HCl, 5 mM MgSO4, 20 mM KCl, pH 7.5, at 30°C
Dirofilaria immitis
5.4.2.12
71
-
in 30 mM Tris-HCl, 5 mM MgSO4, 20 mM KCl, pH 7.5, at 30°C
Wolbachia endosymbiont of Dirofilaria immitis
5.4.2.12
71
-
purified recombinant His-tagged enzyme, pH 7.5, 30°C
Wolbachia sp.
5.4.2.12
225
-
in 30 mM Tris-HCl, 5 mM MgSO4, 20 mM KCl, pH 7.5, at 30°C; purified recombinant His-tagged enzyme, pH 7.5, 30°C
Dirofilaria immitis
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5.4.2.1
2-phospho-D-glycerate
-
716984
Wolbachia endosymbiont of Dirofilaria immitis
3-phospho-D-glycerate
-
-
-
r
5.4.2.1
2-phospho-D-glycerate
-
716984
Dirofilaria immitis
3-phospho-D-glycerate
-
-
-
r
5.4.2.1
3-phospho-D-glycerate
-
716984
Wolbachia endosymbiont of Dirofilaria immitis
2-phospho-D-glycerate
-
-
-
r
5.4.2.1
3-phospho-D-glycerate
-
716984
Dirofilaria immitis
2-phospho-D-glycerate
-
-
-
r
5.4.2.12
2-phospho-D-glycerate
-
716984
Dirofilaria immitis
3-phospho-D-glycerate
-
-
-
r
5.4.2.12
2-phospho-D-glycerate
-
716984
Wolbachia sp.
3-phospho-D-glycerate
-
-
-
r
5.4.2.12
2-phospho-D-glycerate
-
716984
Wolbachia endosymbiont of Dirofilaria immitis
3-phospho-D-glycerate
-
-
-
r
5.4.2.12
3-phospho-D-glycerate
-
716984
Dirofilaria immitis
2-phospho-D-glycerate
-
-
-
r
5.4.2.12
3-phospho-D-glycerate
-
716984
Wolbachia sp.
2-phospho-D-glycerate
-
-
-
r
5.4.2.12
3-phospho-D-glycerate
-
716984
Wolbachia endosymbiont of Dirofilaria immitis
2-phospho-D-glycerate
-
-
-
r
Subunits
EC Number
Subunits
Commentary
Organism
5.4.2.1
?
x * 57000, calculated from amino acid sequence
Dirofilaria immitis
5.4.2.1
?
x * 56000, calculated from amino acid sequence
Wolbachia endosymbiont of Dirofilaria immitis
5.4.2.12
?
x * 57000, calculated from amino acid sequence
Dirofilaria immitis
5.4.2.12
?
x * 56000, calculated from amino acid sequence
Wolbachia endosymbiont of Dirofilaria immitis
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
5.4.2.12
30
-
assay at
Dirofilaria immitis
5.4.2.12
30
-
assay at
Wolbachia sp.
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
5.4.2.1
102
-
3-phospho-D-glycerate
in 30 mM Tris-HCl, 5 mM MgSO4, 20 mM KCl, pH 7.5, at 30°C
Wolbachia endosymbiont of Dirofilaria immitis
5.4.2.1
132
-
3-phospho-D-glycerate
in 30 mM Tris-HCl, 5 mM MgSO4, 20 mM KCl, pH 7.5, at 30°C
Dirofilaria immitis
5.4.2.12
102
-
3-phospho-D-glycerate
in 30 mM Tris-HCl, 5 mM MgSO4, 20 mM KCl, pH 7.5, at 30°C
Wolbachia endosymbiont of Dirofilaria immitis
5.4.2.12
102
-
3-phospho-D-glycerate
recombinant His-tagged enzyme, pH 7.5, 30°C
Wolbachia sp.
5.4.2.12
132
-
3-phospho-D-glycerate
in 30 mM Tris-HCl, 5 mM MgSO4, 20 mM KCl, pH 7.5, at 30°C; recombinant His-tagged enzyme, pH 7.5, 30°C
Dirofilaria immitis
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5.4.2.12
7.5
-
assay at
Dirofilaria immitis
5.4.2.12
7.5
-
assay at
Wolbachia sp.
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
5.4.2.1
additional information
2,3-bisphosphoglycerate is not required as a cofactor
Dirofilaria immitis
5.4.2.1
additional information
2,3-bisphosphoglycerate is not required as a cofactor
Wolbachia endosymbiont of Dirofilaria immitis
5.4.2.12
additional information
2,3-bisphosphoglycerate is not required as a cofactor
Dirofilaria immitis
5.4.2.12
additional information
2,3-bisphosphoglycerate is not required as a cofactor
Wolbachia endosymbiont of Dirofilaria immitis
pI Value
EC Number
Organism
Commentary
pI Value Maximum
pI Value
5.4.2.1
Dirofilaria immitis
calculated from amino acid sequence
-
6.3
5.4.2.1
Wolbachia endosymbiont of Dirofilaria immitis
calculated from amino acid sequence
-
7.4
5.4.2.12
Dirofilaria immitis
calculated from amino acid sequence
-
6.3
5.4.2.12
Wolbachia endosymbiont of Dirofilaria immitis
calculated from amino acid sequence
-
7.4
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
5.4.2.12
additional information
independent on 2,3-bisphosphoglycerate
Dirofilaria immitis
5.4.2.12
additional information
independent on 2,3-bisphosphoglycerate
Wolbachia sp.
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
5.4.2.1
expressed in Escherichia coli C2566/T7 cells
Dirofilaria immitis
5.4.2.1
expressed in Escherichia coli C2566/T7 cells
Wolbachia endosymbiont of Dirofilaria immitis
5.4.2.12
expressed in Escherichia coli C2566/T7 cells
Dirofilaria immitis
5.4.2.12
expressed in Escherichia coli C2566/T7 cells
Wolbachia endosymbiont of Dirofilaria immitis
5.4.2.12
expression of C-terminally His-tagged enzyme in Escherichia coli strain C2566/T7
Wolbachia sp.
5.4.2.12
expression of C-terminally His6-tagged enzyme in Escherichia coli strain C2566/T7
Dirofilaria immitis
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
5.4.2.1
additional information
2,3-bisphosphoglycerate is not required as a cofactor
Dirofilaria immitis
5.4.2.1
additional information
2,3-bisphosphoglycerate is not required as a cofactor
Wolbachia endosymbiont of Dirofilaria immitis
5.4.2.12
additional information
2,3-bisphosphoglycerate is not required as a cofactor
Dirofilaria immitis
5.4.2.12
additional information
2,3-bisphosphoglycerate is not required as a cofactor
Wolbachia endosymbiont of Dirofilaria immitis
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
5.4.2.1
0.55
-
3-phospho-D-glycerate
in 30 mM Tris-HCl, 5 mM MgSO4, 20 mM KCl, pH 7.5, at 30°C
Wolbachia endosymbiont of Dirofilaria immitis
5.4.2.1
0.58
-
3-phospho-D-glycerate
in 30 mM Tris-HCl, 5 mM MgSO4, 20 mM KCl, pH 7.5, at 30°C
Dirofilaria immitis
5.4.2.12
additional information
-
additional information
Michaelis-Menten kinetics
Dirofilaria immitis
5.4.2.12
additional information
-
additional information
Michaelis-Menten kinetics
Wolbachia sp.
5.4.2.12
0.55
-
3-phospho-D-glycerate
in 30 mM Tris-HCl, 5 mM MgSO4, 20 mM KCl, pH 7.5, at 30°C
Wolbachia endosymbiont of Dirofilaria immitis
5.4.2.12
0.55
-
3-phospho-D-glycerate
recombinant His-tagged enzyme, pH 7.5, 30°C
Wolbachia sp.
5.4.2.12
0.58
-
3-phospho-D-glycerate
recombinant His-tagged enzyme, pH 7.5, 30°C
Dirofilaria immitis
5.4.2.12
0.58
-
3-phospho-D-glycerate
in 30 mM Tris-HCl, 5 mM MgSO4, 20 mM KCl, pH 7.5, at 30°C
Dirofilaria immitis
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
5.4.2.1
56000
-
x * 56000, calculated from amino acid sequence
Wolbachia endosymbiont of Dirofilaria immitis
5.4.2.1
57000
-
x * 57000, calculated from amino acid sequence
Dirofilaria immitis
5.4.2.12
56000
-
x * 56000, calculated from amino acid sequence
Wolbachia endosymbiont of Dirofilaria immitis
5.4.2.12
57000
-
x * 57000, calculated from amino acid sequence
Dirofilaria immitis
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
5.4.2.12
2-phospho-D-glycerate
Dirofilaria immitis
-
3-phospho-D-glycerate
-
-
r
5.4.2.12
2-phospho-D-glycerate
Wolbachia sp.
-
3-phospho-D-glycerate
-
-
r
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
5.4.2.1
HiTrap nickel chelating column chromatography and HiTrap Q column chromatography
Dirofilaria immitis
5.4.2.1
HiTrap nickel chelating column chromatography and HiTrap Q column chromatography
Wolbachia endosymbiont of Dirofilaria immitis
5.4.2.12
HiTrap nickel chelating column chromatography and HiTrap Q column chromatography
Dirofilaria immitis
5.4.2.12
HiTrap nickel chelating column chromatography and HiTrap Q column chromatography
Wolbachia endosymbiont of Dirofilaria immitis
5.4.2.12
recombinant His6-tagged enzyme from Escherichia coli strain C2566/T7 by nicke affinity chromatography, dialysis and anion exchange chromatography
Wolbachia sp.
5.4.2.12
recombinant His6-tagged enzyme from Escherichia coli strain C2566/T7 by nickel affinity chromatography, dialysis and anion exchange chromatography
Dirofilaria immitis
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
5.4.2.12
additional information
optimal enzyme production at 37°C
Wolbachia sp.
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
5.4.2.1
71
-
in 30 mM Tris-HCl, 5 mM MgSO4, 20 mM KCl, pH 7.5, at 30°C
Wolbachia endosymbiont of Dirofilaria immitis
5.4.2.1
225
-
in 30 mM Tris-HCl, 5 mM MgSO4, 20 mM KCl, pH 7.5, at 30°C
Dirofilaria immitis
5.4.2.12
71
-
in 30 mM Tris-HCl, 5 mM MgSO4, 20 mM KCl, pH 7.5, at 30°C
Wolbachia endosymbiont of Dirofilaria immitis
5.4.2.12
71
-
purified recombinant His-tagged enzyme, pH 7.5, 30°C
Wolbachia sp.
5.4.2.12
225
-
purified recombinant His-tagged enzyme, pH 7.5, 30°C
Dirofilaria immitis
5.4.2.12
225
-
in 30 mM Tris-HCl, 5 mM MgSO4, 20 mM KCl, pH 7.5, at 30°C
Dirofilaria immitis
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5.4.2.1
2-phospho-D-glycerate
-
716984
Wolbachia endosymbiont of Dirofilaria immitis
3-phospho-D-glycerate
-
-
-
r
5.4.2.1
2-phospho-D-glycerate
-
716984
Dirofilaria immitis
3-phospho-D-glycerate
-
-
-
r
5.4.2.1
3-phospho-D-glycerate
-
716984
Wolbachia endosymbiont of Dirofilaria immitis
2-phospho-D-glycerate
-
-
-
r
5.4.2.1
3-phospho-D-glycerate
-
716984
Dirofilaria immitis
2-phospho-D-glycerate
-
-
-
r
5.4.2.12
2-phospho-D-glycerate
-
716984
Dirofilaria immitis
3-phospho-D-glycerate
-
-
-
r
5.4.2.12
2-phospho-D-glycerate
-
716984
Wolbachia sp.
3-phospho-D-glycerate
-
-
-
r
5.4.2.12
2-phospho-D-glycerate
-
716984
Wolbachia endosymbiont of Dirofilaria immitis
3-phospho-D-glycerate
-
-
-
r
5.4.2.12
3-phospho-D-glycerate
-
716984
Dirofilaria immitis
2-phospho-D-glycerate
-
-
-
r
5.4.2.12
3-phospho-D-glycerate
-
716984
Wolbachia sp.
2-phospho-D-glycerate
-
-
-
r
5.4.2.12
3-phospho-D-glycerate
-
716984
Wolbachia endosymbiont of Dirofilaria immitis
2-phospho-D-glycerate
-
-
-
r
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
5.4.2.1
?
x * 57000, calculated from amino acid sequence
Dirofilaria immitis
5.4.2.1
?
x * 56000, calculated from amino acid sequence
Wolbachia endosymbiont of Dirofilaria immitis
5.4.2.12
?
x * 57000, calculated from amino acid sequence
Dirofilaria immitis
5.4.2.12
?
x * 56000, calculated from amino acid sequence
Wolbachia endosymbiont of Dirofilaria immitis
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
5.4.2.12
30
-
assay at
Dirofilaria immitis
5.4.2.12
30
-
assay at
Wolbachia sp.
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
5.4.2.1
102
-
3-phospho-D-glycerate
in 30 mM Tris-HCl, 5 mM MgSO4, 20 mM KCl, pH 7.5, at 30°C
Wolbachia endosymbiont of Dirofilaria immitis
5.4.2.1
132
-
3-phospho-D-glycerate
in 30 mM Tris-HCl, 5 mM MgSO4, 20 mM KCl, pH 7.5, at 30°C
Dirofilaria immitis
5.4.2.12
102
-
3-phospho-D-glycerate
in 30 mM Tris-HCl, 5 mM MgSO4, 20 mM KCl, pH 7.5, at 30°C
Wolbachia endosymbiont of Dirofilaria immitis
5.4.2.12
102
-
3-phospho-D-glycerate
recombinant His-tagged enzyme, pH 7.5, 30°C
Wolbachia sp.
5.4.2.12
132
-
3-phospho-D-glycerate
recombinant His-tagged enzyme, pH 7.5, 30°C
Dirofilaria immitis
5.4.2.12
132
-
3-phospho-D-glycerate
in 30 mM Tris-HCl, 5 mM MgSO4, 20 mM KCl, pH 7.5, at 30°C
Dirofilaria immitis
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5.4.2.12
7.5
-
assay at
Dirofilaria immitis
5.4.2.12
7.5
-
assay at
Wolbachia sp.
pI Value (protein specific)
EC Number
Organism
Commentary
pI Value Maximum
pI Value
5.4.2.1
Dirofilaria immitis
calculated from amino acid sequence
-
6.3
5.4.2.1
Wolbachia endosymbiont of Dirofilaria immitis
calculated from amino acid sequence
-
7.4
5.4.2.12
Dirofilaria immitis
calculated from amino acid sequence
-
6.3
5.4.2.12
Wolbachia endosymbiont of Dirofilaria immitis
calculated from amino acid sequence
-
7.4
KCat/KM [mM/s]
EC Number
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
5.4.2.1
186
-
3-phospho-D-glycerate
in 30 mM Tris-HCl, 5 mM MgSO4, 20 mM KCl, pH 7.5, at 30°C
Wolbachia endosymbiont of Dirofilaria immitis
5.4.2.1
228
-
3-phospho-D-glycerate
in 30 mM Tris-HCl, 5 mM MgSO4, 20 mM KCl, pH 7.5, at 30°C
Dirofilaria immitis
5.4.2.12
186
-
3-phospho-D-glycerate
recombinant His-tagged enzyme, pH 7.5, 30°C
Wolbachia sp.
5.4.2.12
228
-
3-phospho-D-glycerate
recombinant His-tagged enzyme, pH 7.5, 30°C
Dirofilaria immitis
KCat/KM [mM/s] (protein specific)
EC Number
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
5.4.2.1
186
-
3-phospho-D-glycerate
in 30 mM Tris-HCl, 5 mM MgSO4, 20 mM KCl, pH 7.5, at 30°C
Wolbachia endosymbiont of Dirofilaria immitis
5.4.2.1
228
-
3-phospho-D-glycerate
in 30 mM Tris-HCl, 5 mM MgSO4, 20 mM KCl, pH 7.5, at 30°C
Dirofilaria immitis
5.4.2.12
186
-
3-phospho-D-glycerate
recombinant His-tagged enzyme, pH 7.5, 30°C
Wolbachia sp.
5.4.2.12
228
-
3-phospho-D-glycerate
recombinant His-tagged enzyme, pH 7.5, 30°C
Dirofilaria immitis