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Literature summary extracted from

  • Gomes, M.T.; Guimaraes, G.; Frezard, F.; Kalapothakis, E.; Minozzo, J.C.; Chaim, O.M.; Veiga, S.S.; Oliveira, S.C.; Chavez-Olortegui, C.
    Determination of sphingomyelinase-D activity of Loxosceles venoms in sphingomyelin/cholesterol liposomes containing horseradish peroxidase (2011), Toxicon, 57, 574-579.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.4.41 gene LiD1, expression in Escherichia coli strain BL21(DE3) Loxosceles intermedia

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.4.41 additional information enzyme activity is blocked by the anti-loxoscelic antivenom Loxosceles gaucho
3.1.4.41 additional information enzyme activity is blocked by the anti-loxoscelic antivenom Loxosceles intermedia
3.1.4.41 additional information enzyme activity is blocked by the anti-loxoscelic antivenom Loxosceles laeta
3.1.4.41 additional information enzyme activity is blocked by the anti-loxoscelic antivenom Loxosceles similis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.4.41 extracellular
-
 Loxosceles laeta
-
-
3.1.4.41 extracellular
-
 Loxosceles intermedia
-
-
3.1.4.41 extracellular
-
 Loxosceles gaucho
-
-
3.1.4.41 extracellular
-
 Loxosceles similis
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.4.41 Mg2+ required Loxosceles laeta
3.1.4.41 Mg2+ required Loxosceles intermedia
3.1.4.41 Mg2+ required Loxosceles gaucho
3.1.4.41 Mg2+ required Loxosceles similis

Organism

EC Number Organism UniProt Comment Textmining
3.1.4.41 Loxosceles gaucho
-
-
-
3.1.4.41 Loxosceles intermedia
-
 gene LiD1
-
3.1.4.41 Loxosceles laeta
-
-
-
3.1.4.41 Loxosceles similis
-
-
-
3.1.4.41 no activity in Bothrops jararaca
-
 venom
-
3.1.4.41 no activity in Crotalus durissus
-
 venom
-
3.1.4.41 no activity in Lachesis muta
-
 venom
-
3.1.4.41 no activity in Micrurus frontalis
-
 venom
-
3.1.4.41 no activity in Phoneutria nigriventer
-
 venom
-
3.1.4.41 no activity in Tityus serrulatus
-
 venom
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.4.41 venom
-
 Loxosceles laeta
-
3.1.4.41 venom
-
 Loxosceles intermedia
-
3.1.4.41 venom
-
 Loxosceles gaucho
-
3.1.4.41 venom
-
 Loxosceles similis
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.4.41 sphingomyelin + H2O substrate as sphingomyelin/cholesterol multilamellar liposomes in a molar ratio of 2:1 Loxosceles laeta ceramide 1-phosphate + choline
-
 ?
3.1.4.41 sphingomyelin + H2O substrate as sphingomyelin/cholesterol multilamellar liposomes in a molar ratio of 2:1 Loxosceles intermedia ceramide 1-phosphate + choline
-
 ?
3.1.4.41 sphingomyelin + H2O substrate as sphingomyelin/cholesterol multilamellar liposomes in a molar ratio of 2:1 Loxosceles gaucho ceramide 1-phosphate + choline
-
 ?
3.1.4.41 sphingomyelin + H2O substrate as sphingomyelin/cholesterol multilamellar liposomes in a molar ratio of 2:1 Loxosceles similis ceramide 1-phosphate + choline
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.4.41 SMase-D
-
 Loxosceles laeta
3.1.4.41 SMase-D
-
 Loxosceles intermedia
3.1.4.41 SMase-D
-
 Loxosceles gaucho
3.1.4.41 SMase-D
-
 Loxosceles similis
3.1.4.41 sphingomyelinase-D
-
 Loxosceles laeta
3.1.4.41 sphingomyelinase-D
-
 Loxosceles intermedia
3.1.4.41 sphingomyelinase-D
-
 Loxosceles gaucho
3.1.4.41 sphingomyelinase-D
-
 Loxosceles similis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.4.41 37
-
 assay at Loxosceles laeta
3.1.4.41 37
-
 assay at Loxosceles intermedia
3.1.4.41 37
-
 assay at Loxosceles gaucho
3.1.4.41 37
-
 assay at Loxosceles similis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.4.41 7.4
-
 assay at Loxosceles laeta
3.1.4.41 7.4
-
 assay at Loxosceles intermedia
3.1.4.41 7.4
-
 assay at Loxosceles gaucho
3.1.4.41 7.4
-
 assay at Loxosceles similis