Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Huang, S.; Liu, R.; Niu, Y.; Hasi, A.
    Cloning and functional characterization of a fatty acid DELTA6-desaturase from Oenothera biennis: Production of alpha-linolenic acid by heterologous expression in Saccharomyces cerevisiae (2010), Russ. J. Plant Physiol., 57, 568-573.
No PubMed abstract available

Application

EC Number Application Comment Organism
1.14.19.3 synthesis Oenothera biennis is grown commercially for gamma-linolenic acid production by the fatty acid DELTA6-desaturase enzymatic reaction Oenothera biennis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.19.3 gene ObD6Des, DNA and amino acid sequence determination and analysis, sequence comparisons, functional expression in Saccharomyces cerevisiae strain W303-a Oenothera biennis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.19.3 alpha-linoleoyl-CoA + AH2 + O2 Oenothera biennis
-
 stearidonoyl-CoA + A + 2 H2O
-
 ?
1.14.19.3 linoleoyl-CoA + AH2 + O2 Oenothera biennis
-
 gamma-linolenoyl-CoA + A + 2 H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.19.3 Oenothera biennis E1U2Q3 gene ObD6Des
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.19.3 alpha-linoleoyl-CoA + AH2 + O2
-
 Oenothera biennis stearidonoyl-CoA + A + 2 H2O
-
 ?
1.14.19.3 linoleoyl-CoA + AH2 + O2
-
 Oenothera biennis gamma-linolenoyl-CoA + A + 2 H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.19.3 More the enzyme sequence comprises the characteristics of membrane-bound desaturases, including three conserved histidine-rich motifs, hydropathy profile, and a cytochrome b5 domain at the N-terminus, overview Oenothera biennis

Synonyms

EC Number Synonyms Comment Organism
1.14.19.3 D6Des
-
 Oenothera biennis
1.14.19.3 fatty acid DELTA6-desaturase
-
 Oenothera biennis

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.19.3 cytochrome b5 binds at the N-terminus of the enzyme Oenothera biennis

General Information

EC Number General Information Comment Organism
1.14.19.3 physiological function fatty acid DELTA6-desaturase is the essential enzyme for polyunsaturated fatty acids biosynthesis and is responsible for the first step by converting linoleic acid to gamma-linolenoyl-CoA, and alpha-linolenic acid to stearidonic acid Oenothera biennis