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Literature summary extracted from

  • Yamazaki, M.; Asano, T.; Yamazaki, Y.; Sirikantaramas, S.; Sudo, H.; Saito, K.
    Biosynthetic system of camptothecin: An anticancer plant product (2010), Pure Appl. Chem., 82, 213-218.
No PubMed abstract available

Application

EC Number Application Comment Organism
4.3.3.2 synthesis production of campothecin in hairy root culture of Ophiorrhiza pumila by infection with Agrobacterium tumefaciens. The culture also produces a high level of anthraquinones besides camptothecin. Camptothecin is present not only in hairy root cells, but excreted out to medium at a substantial amount. Camptothecin content in the medium is increased by the presence of a polystyrene resin that absorbs camptothecin Ophiorrhiza pumila

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.3.3.2
-
 Ophiorrhiza pumila

Protein Variants

EC Number Protein Variants Comment Organism
4.3.3.2 C97S loss of activity Ophiorrhiza pumila
4.3.3.2 D213E loss of activity Ophiorrhiza pumila
4.3.3.2 D213N loss of activity Ophiorrhiza pumila
4.3.3.2 E194D about 30% of wild-type activity Ophiorrhiza pumila
4.3.3.2 E194V about 55% of wild-type activity Ophiorrhiza pumila
4.3.3.2 H306L loss of activity Ophiorrhiza pumila
4.3.3.2 H317L loss of activity Ophiorrhiza pumila
4.3.3.2 K144L about 124% of wild-type activity Ophiorrhiza pumila
4.3.3.2 K200L loss of activity Ophiorrhiza pumila
4.3.3.2 K200R about 90% of wild-type activity Ophiorrhiza pumila
4.3.3.2 K236L loss of activity Ophiorrhiza pumila
4.3.3.2 K236R loss of activity Ophiorrhiza pumila
4.3.3.2 N251S about 115% of wild-type activity Ophiorrhiza pumila
4.3.3.2 N88S loss of activity Ophiorrhiza pumila
4.3.3.2 Y63F about 50% of wild-type activity Ophiorrhiza pumila
4.3.3.2 Y63L about 50% of wild-type activity Ophiorrhiza pumila

Organism

EC Number Organism UniProt Comment Textmining
4.3.3.2 Ophiorrhiza pumila Q94LW9
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.3.3.2 hairy root hairy root and root, high expression Ophiorrhiza pumila
-
4.3.3.2 root hairy root and root, high expression Ophiorrhiza pumila
-
4.3.3.2 stem high expression Ophiorrhiza pumila
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.3.3.2 4-methyltryptamine + secologanin poor substrate Ophiorrhiza pumila ? + H2O
-
 ?
4.3.3.2 5-hydroxytryptamine + secologanin poor substrate Ophiorrhiza pumila ? + H2O
-
 ?
4.3.3.2 5-methoxytryptamine + secologanin poor substrate Ophiorrhiza pumila ? + H2O
-
 ?
4.3.3.2 6-methoxytryptamine + secologanin poor substrate Ophiorrhiza pumila ? + H2O
-
 ?
4.3.3.2 7-methyltryptamine + secologanin poor substrate Ophiorrhiza pumila ? + H2O
-
 ?
4.3.3.2 tryptamine + secologanin poor substrate Ophiorrhiza pumila ? + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.3.3.2 str
-
 Ophiorrhiza pumila