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Literature summary extracted from

  • Labbe, G.; de Groot, S.; Rasmusson, T.; Milojevic, G.; Dmitrienko, G.I.; Guillemette, J.G.
    Evaluation of four microbial Class II fructose 1,6-bisphosphate aldolase enzymes for use as biocatalysts (2011), Protein Expr. Purif., 80, 224-233.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.2.13 expressed in Escherichia coli BL21(DE3) cells Pseudomonas aeruginosa
4.1.2.13 expressed in Escherichia coli BL21(DE3) cells Bacillus cereus
4.1.2.13 expressed in Escherichia coli BL21(DE3) cells Mycobacterium tuberculosis
4.1.2.13 expressed in Escherichia coli BL21(DE3) cells Pyricularia grisea

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.2.13 EDTA the specific activity of the Bacillus cereus aldolase is partially restored after EDTA inactivation by Co2+ and Cd2+, as well as Zn2+, but not significantly restored by Cu2+, Mn2+, Mg2+, or Ni2+ Bacillus cereus
4.1.2.13 EDTA the activity of the EDTA-inactivated enzyme increases more than 6fold upon the addition of 0.002 mM Zn2+ and increases by a factor 2 with the addition of 0.002 mM Co2+. The addition of Cu2+, Ni2+, Cd2+, Mn2+, or Mg2+ does not reactivate the enzyme significantly Mycobacterium tuberculosis
4.1.2.13 EDTA the specific activity of the recombinant Pseudomonas aeruginosa aldolase is 5.5times higher after inactivation by EDTA followed by the addition of 0.02 mM cobalt chloride, and increases more than 2fold after EDTA inactivation followed by the addition of 0.1 mM manganese(II)-chloride Pseudomonas aeruginosa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.2.13 0.0279
-
 D-fructose 1,6-bisphosphate in Tris-HCl pH 8.0, at 30°C Mycobacterium tuberculosis
4.1.2.13 0.035
-
 D-fructose 1,6-bisphosphate in Tris-HCl pH 8.0, at 30°C Pseudomonas aeruginosa
4.1.2.13 0.051
-
 D-fructose 1,6-bisphosphate in Tris-HCl pH 8.0, at 30°C Pyricularia grisea
4.1.2.13 0.45
-
 D-fructose 1,6-bisphosphate in Tris-HCl pH 8.0, at 30°C Bacillus cereus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.2.13 additional information the addition of Co2+ to the purified Bacillus cereus aldolase does not increase the relatively low specific activity of this recombinant enzyme Bacillus cereus
4.1.2.13 Zn2+ contains 0.16 zinc atoms per subunit Pseudomonas aeruginosa
4.1.2.13 Zn2+ contains 0.49 zinc atoms per subunit Mycobacterium tuberculosis
4.1.2.13 Zn2+ contains 0.83 zinc atoms per subunit Pyricularia grisea
4.1.2.13 Zn2+ contains 0.97 zinc atoms per subunit Bacillus cereus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.2.13 30540
-
 1 * 30671 + 1 * 30540, mass spectrometry Bacillus cereus
4.1.2.13 30671
-
 1 * 30671 + 1 * 30540, mass spectrometry Bacillus cereus
4.1.2.13 36411
-
 4 * 36411, mass spectrometry Mycobacterium tuberculosis
4.1.2.13 38441
-
 4 * 38441, mass spectrometry Pseudomonas aeruginosa
4.1.2.13 39644
-
 1 * 39775 + 1 * 39644, mass spectrometry Pyricularia grisea
4.1.2.13 39775
-
 1 * 39775 + 1 * 39644, mass spectrometry Pyricularia grisea

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
4.1.2.13 Acetone more than 50% of the enzyme activity is retained in the presence of 10% (v/v) acetone Pyricularia grisea
4.1.2.13 Acetone more than 50% of the enzyme activity is retained in the presence of 25% (v/v) acetone Pseudomonas aeruginosa
4.1.2.13 Acetone more than 50% of the enzyme activity is retained in the presence of 25% (v/v) acetone Bacillus cereus
4.1.2.13 Acetone more than 50% of the enzyme activity is retained in the presence of 25% (v/v) acetone Mycobacterium tuberculosis
4.1.2.13 acetonitrile more than 50% of the enzyme activity is retained in the presence of 10% (v/v) acetonitrile Bacillus cereus
4.1.2.13 acetonitrile more than 50% of the enzyme activity is retained in the presence of 10% (v/v) acetonitrile Mycobacterium tuberculosis
4.1.2.13 acetonitrile more than 50% of the enzyme activity is retained in the presence of 15% (v/v) acetonitrile Pseudomonas aeruginosa
4.1.2.13 acetonitrile more than 50% of the enzyme activity is retained in the presence of about 5% (v/v) acetonitrile Pyricularia grisea
4.1.2.13 dimethylformamide more than 50% of the enzyme activity is retained in the presence of 10% (v/v) dimethylformamide Pyricularia grisea
4.1.2.13 dimethylformamide more than 50% of the enzyme activity is retained in the presence of 20% (v/v) dimethylformamide Mycobacterium tuberculosis
4.1.2.13 dimethylformamide more than 50% of the enzyme activity is retained in the presence of 35% (v/v) dimethylformamide Pseudomonas aeruginosa
4.1.2.13 dimethylformamide more than 50% of the enzyme activity is retained in the presence of 50% (v/v) dimethylformamide Bacillus cereus
4.1.2.13 DMSO 167% activity in the presence of 50% (v/v) DMSO Bacillus cereus
4.1.2.13 DMSO more than 50% of the enzyme activity is retained in the presence of 20% (v/v) DMSO Pyricularia grisea
4.1.2.13 DMSO more than 50% of the enzyme activity is retained in the presence of 50% (v/v) DMSO Pseudomonas aeruginosa
4.1.2.13 DMSO more than 50% of the enzyme activity is retained in the presence of 50% (v/v) DMSO Mycobacterium tuberculosis
4.1.2.13 tert-Butanol more than 50% of the enzyme activity is retained in the presence of 15% (v/v) tert-butanol Pseudomonas aeruginosa
4.1.2.13 tert-Butanol more than 50% of the enzyme activity is retained in the presence of 20% (v/v) tert-butanol Mycobacterium tuberculosis
4.1.2.13 tert-Butanol more than 50% of the enzyme activity is retained in the presence of about 5% (v/v) tert-butanol Bacillus cereus
4.1.2.13 tert-Butanol more than 50% of the enzyme activity is retained in the presence of about 5% (v/v) tert-butanol Pyricularia grisea

Organism

EC Number Organism UniProt Comment Textmining
4.1.2.13 Bacillus cereus
-
-
-
4.1.2.13 Bacillus cereus ATCC 10987
-
-
-
4.1.2.13 Mycobacterium tuberculosis
-
-
-
4.1.2.13 Pseudomonas aeruginosa
-
-
-
4.1.2.13 Pyricularia grisea
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.2.13 ammonium sulfate precipitation, DEAE Sepharose column chromatography, and Sepharose Q column chromatography Pseudomonas aeruginosa
4.1.2.13 ammonium sulfate precipitation, DEAE Sepharose column chromatography, and Sepharose Q column chromatography Bacillus cereus
4.1.2.13 ammonium sulfate precipitation, DEAE Sepharose column chromatography, and Sepharose Q column chromatography Mycobacterium tuberculosis
4.1.2.13 ammonium sulfate precipitation, DEAE Sepharose column chromatography, and Sepharose Q column chromatography Pyricularia grisea

Storage Stability

EC Number Storage Stability Organism
4.1.2.13 4°C, in 50 mM HEPES buffer with pH 7.3 supplemented with ZnCl2, 24 h, 40% loss of activity Bacillus cereus
4.1.2.13 4°C, in 50 mM HEPES buffer with pH 7.3 supplemented with ZnCl2, several days, the enzyme remains stable Pseudomonas aeruginosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.2.13 D-fructose 1,6-bisphosphate
-
 Pseudomonas aeruginosa dihydroxyacetone phosphate + D-glyceraldehyde 3-phosphate
-
 r
4.1.2.13 D-fructose 1,6-bisphosphate
-
 Bacillus cereus dihydroxyacetone phosphate + D-glyceraldehyde 3-phosphate
-
 r
4.1.2.13 D-fructose 1,6-bisphosphate
-
 Mycobacterium tuberculosis dihydroxyacetone phosphate + D-glyceraldehyde 3-phosphate
-
 r
4.1.2.13 D-fructose 1,6-bisphosphate
-
 Pyricularia grisea dihydroxyacetone phosphate + D-glyceraldehyde 3-phosphate
-
 r
4.1.2.13 D-fructose 1,6-bisphosphate
-
 Bacillus cereus ATCC 10987 dihydroxyacetone phosphate + D-glyceraldehyde 3-phosphate
-
 r

Subunits

EC Number Subunits Comment Organism
4.1.2.13 heterodimer 1 * 30671 + 1 * 30540, mass spectrometry Bacillus cereus
4.1.2.13 heterodimer 1 * 39775 + 1 * 39644, mass spectrometry Pyricularia grisea
4.1.2.13 homotetramer 4 * 36411, mass spectrometry Mycobacterium tuberculosis
4.1.2.13 homotetramer 4 * 38441, mass spectrometry Pseudomonas aeruginosa

Synonyms

EC Number Synonyms Comment Organism
4.1.2.13 class II fructose 1,6-bisphosphate aldolase
-
 Pseudomonas aeruginosa
4.1.2.13 class II fructose 1,6-bisphosphate aldolase
-
 Bacillus cereus
4.1.2.13 class II fructose 1,6-bisphosphate aldolase
-
 Mycobacterium tuberculosis
4.1.2.13 class II fructose 1,6-bisphosphate aldolase
-
 Pyricularia grisea
4.1.2.13 FBP aldolase
-
 Pseudomonas aeruginosa
4.1.2.13 FBP aldolase
-
 Bacillus cereus
4.1.2.13 FBP aldolase
-
 Mycobacterium tuberculosis
4.1.2.13 FBP aldolase
-
 Pyricularia grisea

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.1.2.13 45 60 the aldolase loses 25% of its activity after 10 min of incubation at 45°C and loses almost all its activity after a10 min incubation at 60°C Pyricularia grisea
4.1.2.13 60
-
 the aldolase loses almost all its activity after a 10 min incubation at 60°C Mycobacterium tuberculosis
4.1.2.13 60
-
 the aldolase loses almost all its activity after a10 min incubation at 60°C Pseudomonas aeruginosa
4.1.2.13 90
-
 the aldolase retains almost half of its activity after 10 min of incubation at 90°C Bacillus cereus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.1.2.13 1.53
-
 D-fructose 1,6-bisphosphate in Tris-HCl pH 8.0, at 30°C Pseudomonas aeruginosa
4.1.2.13 2.95
-
 D-fructose 1,6-bisphosphate in Tris-HCl pH 8.0, at 30°C Bacillus cereus
4.1.2.13 30.1
-
 D-fructose 1,6-bisphosphate in Tris-HCl pH 8.0, at 30°C Mycobacterium tuberculosis
4.1.2.13 45.7
-
 D-fructose 1,6-bisphosphate in Tris-HCl pH 8.0, at 30°C Pyricularia grisea

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.2.13 7.8
-
-
 Mycobacterium tuberculosis
4.1.2.13 7.8
-
-
 Pyricularia grisea
4.1.2.13 8
-
-
 Bacillus cereus
4.1.2.13 8.5
-
-
 Pseudomonas aeruginosa

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4.1.2.13 0.066
-
 D-fructose 1,6-bisphosphate in Tris-HCl pH 8.0, at 30°C Bacillus cereus
4.1.2.13 0.44
-
 D-fructose 1,6-bisphosphate in Tris-HCl pH 8.0, at 30°C Pseudomonas aeruginosa
4.1.2.13 9
-
 D-fructose 1,6-bisphosphate in Tris-HCl pH 8.0, at 30°C Pyricularia grisea
4.1.2.13 10.8
-
 D-fructose 1,6-bisphosphate in Tris-HCl pH 8.0, at 30°C Mycobacterium tuberculosis