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Literature summary extracted from

  • Singh, P.K.; Chan, P.F.; Hibbs, M.J.; Vazquez, M.J.; Segura, D.C.; Thomas, D.A.; Theobald, A.J.; Gallagher, K.T.; Hassan, N.J.
    High-yield production and characterization of biologically active GST-tagged human topoisomerase IIalpha protein in insect cells for the development of a high-throughput assay (2011), Protein Expr. Purif., 76, 165-172.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.6.2.2 expression of GST-tagged isozyme topo2alpha in Spodoptera frugiperda Sf9 cells using the baculovirus transfection system Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.6.2.2 Ciprofloxacin a topoisomerase II inhibitor Homo sapiens
5.6.2.2 etoposide a topoisomerase IIalpha inhibitor Homo sapiens
5.6.2.2 quercetin a topoisomerase IIalpha inhibitor Homo sapiens
5.6.2.2 suramin a topoisomerase IIalpha inhibitor Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.6.2.2 0.0000012
-
 Supercoiled DNA pH 8.0, temperature not specified in the publication, recombinant topo2alpha Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
5.6.2.2 nucleus
-
 Homo sapiens 5634
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
5.6.2.2 Mg2+ required Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
5.6.2.2 Homo sapiens P11388 topoisomerase IIalpha
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.6.2.2 recombinant GST-tagged isozyme topo2alpha from Sf9 insect cells by nuclei preparation, glutathione affinity chromatography, and gel filtration Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.6.2.2 supercoiled DNA + ATP + H2O substrate is supercoiled scpBR322 plasmid DNA. Topo2a creates transient breaks in supercoiled DNA in an ATP-dependent manner resulting in DNA relaxation Homo sapiens ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
5.6.2.2 topo2alpha
-
 Homo sapiens
5.6.2.2 topoisomerase IIalpha
-
 Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.6.2.2 8
-
 DNA relaxation and ATPase assays at Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
5.6.2.2 ATP dependent on Homo sapiens

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
5.6.2.2 0.0003
-
 inhibition of DNA relaxation activity, pH 8.0, temperature not specified in the publication, recombinant topo2alpha Homo sapiens suramin
5.6.2.2 0.0036
-
 inhibition of ATPase activity, pH 8.0, temperature not specified in the publication, recombinant topo2alpha Homo sapiens quercetin
5.6.2.2 0.021
-
 inhibition of ATPase activity, pH 8.0, temperature not specified in the publication, recombinant topo2alpha Homo sapiens suramin
5.6.2.2 0.021
-
 inhibition of ATPase activity, pH 8.0, temperature not specified in the publication, recombinant topo2alpha Homo sapiens etoposide
5.6.2.2 0.0277
-
 inhibition of DNA relaxation activity, pH 8.0, temperature not specified in the publication, recombinant topo2alpha Homo sapiens quercetin
5.6.2.2 0.0589
-
 inhibition of DNA relaxation activity, pH 8.0, temperature not specified in the publication, recombinant topo2alpha Homo sapiens etoposide
5.6.2.2 0.1695
-
 inhibition of DNA relaxation activity, pH 8.0, temperature not specified in the publication, recombinant topo2alpha Homo sapiens Ciprofloxacin
5.6.2.2 0.797
-
 inhibition of ATPase activity, pH 8.0, temperature not specified in the publication, recombinant topo2alpha Homo sapiens Ciprofloxacin