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Literature summary extracted from

  • Soulimane, T.
    Thermus thermophilus encodes an archaeal-like fructose-1,6-bisphosphatase: purification of native and recombinant protein for structural studies (2010), Protein Expr. Purif., 74, 175-180.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.3.11 recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) pLysS Thermus thermophilus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.1.3.11 purified recombinant His-tagged enzyme, sitting drop vapour diffusion method, 20°C, X-ray diffraction structure determination and analysis of twinned crystals at 1.2 A resolution, and of untwinned crystals at 1.8 A resolution Thermus thermophilus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.11 40324
-
 2 * 40324, mass spectrometry, 2 * 40361, sequence calculation Thermus thermophilus
3.1.3.11 40361
-
 2 * 40324, mass spectrometry, 2 * 40361, sequence calculation Thermus thermophilus
3.1.3.11 80000
-
 gel filtration Thermus thermophilus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.3.11 D-fructose 1,6-bisphosphate + H2O Thermus thermophilus
-
 D-fructose 6-phosphate + phosphate
-
 ?
3.1.3.11 D-fructose 1,6-bisphosphate + H2O Thermus thermophilus HB8 / ATCC 27634 / DSM 579
-
 D-fructose 6-phosphate + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.11 Thermus thermophilus Q5SJM8 ATCC 27634
-
3.1.3.11 Thermus thermophilus HB8 / ATCC 27634 / DSM 579 Q5SJM8 ATCC 27634
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.11 recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) pLysS by heat treatment, nickel affinity chromatography, and gel filtration Thermus thermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.11 D-fructose 1,6-bisphosphate + H2O
-
 Thermus thermophilus D-fructose 6-phosphate + phosphate
-
 ?
3.1.3.11 D-fructose 1,6-bisphosphate + H2O
-
 Thermus thermophilus HB8 / ATCC 27634 / DSM 579 D-fructose 6-phosphate + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.11 dimer 2 * 40324, mass spectrometry, 2 * 40361, sequence calculation Thermus thermophilus
3.1.3.11 More in solution, the enzyme appears to be a homodimer which ensembles into an octamer in the tetragonal crystals Thermus thermophilus

Synonyms

EC Number Synonyms Comment Organism
3.1.3.11 archaeal-like fructose-1,6-bisphosphatase
-
 Thermus thermophilus
3.1.3.11 FBPase
-
 Thermus thermophilus
3.1.3.11 fructose-1,6-bisphosphatase
-
 Thermus thermophilus