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Literature summary extracted from

  • Lu, G.; Zhang, J.; Li, Y.; Li, Z.; Zhang, N.; Xu, X.; Wang, T.; Guan, Z.; Gao, G.F.; Yan, J.
    hNUDT16: a universal decapping enzyme for small nucleolar RNA and cytoplasmic mRNA (2011), Protein Cell, 2, 64-73.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.6.1.62 overexpressed in Escherichia coli Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
3.6.1.62 E76Q mutation totally abolishes the decapping activity under the standard assaying condition Homo sapiens
3.6.1.62 E79Q mutation decreases the decapping activity of the enzyme Homo sapiens
3.6.1.62 E80Q mutation totally abolishes the decapping activity under the standard assaying condition Homo sapiens
3.6.1.62 R75L the mutant displays a weaker capability of hydrolysis Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.6.1.62 cytoplasm
-
 Homo sapiens 5737
-
3.6.1.62 nucleus
-
 Homo sapiens 5634
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.6.1.62 Co2+ confer only limited hydrolytic capability on hNUDT16. After 30 min incubation of the RNA substrate with the enzyme, only tiny amounts of m7GDP product are observed on the migration profiles Homo sapiens
3.6.1.62 Mg2+ hNUDT16 relies on divalent cations for its cap-hydrolysis activity to remove m7GDP and m227GDP from RNAs. hNUDT16 without the coordination of metals can not catalyze the hydrolytic reaction since no detectable cleaved products can be observed for either the U8 snoRNA or the mRNA substrate. Both Mg2+ and Mn2+ can effectively switch the protein from apoenzyme to holoenzyme. Mn2+ is more efficient as the activating factor Homo sapiens
3.6.1.62 Mn2+ hNUDT16 relies on divalent cations for its cap-hydrolysis activity to remove m7GDP and m227GDP from RNAs. hNUDT16 without the coordination of metals can not catalyze the hydrolytic reaction since no detectable cleaved products can be observed for either the U8 snoRNA or the mRNA substrate. Both Mg2+ and Mn2+ can effectively switch the protein from apoenzyme to holoenzyme. Mn2+ is more efficient as the activating factor Homo sapiens
3.6.1.62 Zn2+ confer only limited hydrolytic capability on hNUDT16. After 30 min incubation of the RNA substrate with the enzyme, only tiny amounts of m7GDP product are observed on the migration profiles Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.6.1.62 m7G5'ppp5'-mRNA + H2O Homo sapiens hNUDT16 relies on divalent cations for its cap-hydrolysis activity to remove m7GDP and m227GDP from RNAs m7GDP + 5'-phospho-mRNA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.6.1.62 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.6.1.62
-
 Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.1.62 5'-(N7-methylguanosine 5'-triphospho)-mRNA + H2O luciferase mRNA Homo sapiens m7GDP + 5'-phospho-mRNA
-
 ?
3.6.1.62 m7G5'ppp5'-mRNA + H2O hNUDT16 relies on divalent cations for its cap-hydrolysis activity to remove m7GDP and m227GDP from RNAs Homo sapiens m7GDP + 5'-phospho-mRNA
-
 ?
3.6.1.62 m7G5'ppp5'-U8 snomRNA + H2O hNUDT16 relies on divalent cations for its cap-hydrolysis activity to remove m7GDP and m227GDP from RNAs. hNUDT16 without the coordination of metals can not catalyze the hydrolytic reaction since no detectable cleaved products can be observed for either the U8 snoRNA or the mRNA substrate. Both Mg2+ and Mn2+ can effectively switch the protein from apoenzyme to holoenzyme. Mn2+ is more efficient as the activating factor Homo sapiens m7GDP + 5'-phospho-U8 snomRNA
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.6.1.62 hNUDT16
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.6.1.62 37
-
 assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.6.1.62 8
-
 assay at Homo sapiens