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Literature summary extracted from
- Purification and characterization of phosphodiesterase I from Walterinnesia aegyptia venom (2011), Prep. Biochem. Biotechnol., 41, 262-277.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.15.1 | ADP | competitive inhibition | Walterinnesia aegyptia |  |
| 3.1.15.1 | cysteine | noncompetitive inhibition | Walterinnesia aegyptia | |
| 3.1.15.1 | EDTA | strong inhibition, addition of 10 mM Ca2+ restored only 8% of the enzyme activity | Walterinnesia aegyptia | |
| 3.1.15.1 | glutathione | - |
Walterinnesia aegyptia | |
| 3.1.15.1 | o-phenanthroline | - |
Walterinnesia aegyptia | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.1.15.1 | extracellular | - |
Walterinnesia aegyptia | - |
- |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.15.1 | Mg2+ | required, 13% activation at 15-20 mM | Walterinnesia aegyptia | |
| 3.1.15.1 | additional information | the enzyme is active in the absence of any divalent ions and the addition of Ca2+ or Mg2+ has no significant effect on the activity of Walterinnesia aegyptia PDE | Walterinnesia aegyptia |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.1.15.1 | 158000 | - |
x * 158000, SDS-PAGE | Walterinnesia aegyptia |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.15.1 | additional information | Walterinnesia aegyptia | the enzyme sequentially removes mononucleotide units from the polynucleotide chain starting from the free 3' hydroxyl end, and can attack DNA, RNA, or any nucleotide chain | ? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.15.1 | Walterinnesia aegyptia | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.15.1 | native enzyme 3fold from venom by preparative native polyacrylamide gel electrophoresis | Walterinnesia aegyptia |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.1.15.1 | venom | - |
Walterinnesia aegyptia | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.1.15.1 | 3.4 | - |
purified native enzyme, pH 9.0, 37°C | Walterinnesia aegyptia |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.15.1 | ATP + H2O | - |
Walterinnesia aegyptia | ? | - |
? | |
| 3.1.15.1 | cAMP + H2O | very low activity | Walterinnesia aegyptia | ? | - |
? | |
| 3.1.15.1 | additional information | the enzyme sequentially removes mononucleotide units from the polynucleotide chain starting from the free 3' hydroxyl end, and can attack DNA, RNA, or any nucleotide chain | Walterinnesia aegyptia | ? | - |
? | |
| 3.1.15.1 | thymidine-5'-phosphate 4-nitrophenyl ester + H2O | - |
Walterinnesia aegyptia | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.1.15.1 | ? | x * 158000, SDS-PAGE | Walterinnesia aegyptia |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.1.15.1 | PDE-I | - |
Walterinnesia aegyptia |
| 3.1.15.1 | venom exonuclease I | - |
Walterinnesia aegyptia |
| 3.1.15.1 | venom PDE-I | - |
Walterinnesia aegyptia |
| 3.1.15.1 | venom phosphodiesterase I | - |
Walterinnesia aegyptia |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.15.1 | 60 | - |
- |
Walterinnesia aegyptia |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.15.1 | 20 | 90 | assay range, rapid loss of activity above 65°C | Walterinnesia aegyptia |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.15.1 | 7.5 | 9 | assay at, dependent on the substrate | Walterinnesia aegyptia |
Ki Value [mM]
| EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.1.15.1 | 0.8 | - |
ADP | pH pH 9.0, 37°C | Walterinnesia aegyptia | |
| 3.1.15.1 | 6.2 | - |
cysteine | pH pH 9.0, 37°C | Walterinnesia aegyptia | |
pI Value
| EC Number | Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|---|
| 3.1.15.1 | Walterinnesia aegyptia | isoelectric focusing | - |
7.48 |
IC50 Value
| EC Number | IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|---|
| 3.1.15.1 | 2.6 | - |
pH pH 9.0, 37°C | Walterinnesia aegyptia | cysteine | |
| 3.1.15.1 | 8.3 | - |
pH pH 9.0, 37°C | Walterinnesia aegyptia | ADP | |
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