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Literature summary extracted from

  • Molina-Rueda, J.J.; Pascual, M.B.; Canovas, F.M.; Gallardo, F.
    Characterization and developmental expression of a glutamate decarboxylase from maritime pine (2010), Planta, 232, 1471-1483.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.1.15 expressed in Escherichia coli BL21-codonPlus-(DE3)-RIL cells Pinus pinaster

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.1.15 Ca2+ the addition of Ca2+ to the reaction mix causes an activation of GAD activity of 32% Pinus pinaster

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.1.15 56000
-
 x * 56000, SDS-PAGE Pinus pinaster
4.1.1.15 57200
-
 x * 57200, calculated from amino acid sequence Pinus pinaster

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.15 Pinus pinaster A0EJ89
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.15 Ni-NTA column chromatography Pinus pinaster

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.1.1.15 cotyledon
-
 Pinus pinaster
-
4.1.1.15 embryo
-
 Pinus pinaster
-
4.1.1.15 hypocotyl
-
 Pinus pinaster
-
4.1.1.15 root highest expression Pinus pinaster
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.15 L-glutamate
-
 Pinus pinaster 4-aminobutanoate + CO2
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.1.15 ? x * 56000, SDS-PAGE Pinus pinaster
4.1.1.15 ? x * 57200, calculated from amino acid sequence Pinus pinaster

Synonyms

EC Number Synonyms Comment Organism
4.1.1.15 GAD
-
 Pinus pinaster

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.1.15 5.8
-
-
 Pinus pinaster

Cofactor

EC Number Cofactor Comment Organism Structure
4.1.1.15 Calmodulin GAD can be regulated in vivo by Ca2+/calmodulin atpH 5.8. The addition of bovine calmodulin and Ca2+ to the reaction mix causes an activation of GAD activity of 32% Pinus pinaster
4.1.1.15 pyridoxal 5'-phosphate
-
 Pinus pinaster

pI Value

EC Number Organism Comment pI Value Maximum pI Value
4.1.1.15 Pinus pinaster calculated from amino acid sequence
-
 5.7

Expression

EC Number Organism Comment Expression
4.1.1.15 Pinus pinaster GAD expression is induced in response to the production of compression wood and GAD increases during day 14 and 82 after germination in the hypocotyl up

General Information

EC Number General Information Comment Organism
4.1.1.15 physiological function GAD plays a role in hypocotyl and stem development in pine Pinus pinaster