Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Wang, L.; Li, Z.; Qian, W.; Guo, W.; Gao, X.; Huang, L.; Wang, H.; Zhu, H.; Wu, J.W.; Wang, D.; Liu, D.
    Arabidopsis purple acid phosphatase AtPAP10 is predominantly associated with the root surface and plays an important role in plant tolerance to phosphate limitation (2011), Plant Physiol., 157, 1283-99.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.3.2 expressed in Escherichia coli as a GST-fusion protein Arabidopsis thaliana

Protein Variants

EC Number Protein Variants Comment Organism
3.1.3.2 A385V mutation in metallophosphatase domain results in complete loss of enzymatic activity Arabidopsis thaliana
3.1.3.2 D167N mutation in metallophosphatase domain results in complete loss of enzymatic activity Arabidopsis thaliana
3.1.3.2 E340K mutation in metallophosphatase domain results in complete loss of enzymatic activity Arabidopsis thaliana
3.1.3.2 E363K mutation in metallophosphatase domain results in complete loss of enzymatic activity Arabidopsis thaliana
3.1.3.2 G169E mutation in metallophosphatase domain results in complete loss of enzymatic activity Arabidopsis thaliana
3.1.3.2 G195D mutation in metallophosphatase domain results in complete loss of enzymatic activity Arabidopsis thaliana
3.1.3.2 G27E mutation not in metallophosphatase domain results in partial loss of enzymatic activity Arabidopsis thaliana
3.1.3.2 G420R mutation in metallophosphatase domain results in complete loss of enzymatic activity Arabidopsis thaliana
3.1.3.2 G55D mutation not metallophosphatase domain results in partial loss of enzymatic activity Arabidopsis thaliana
3.1.3.2 G67R mutation not metallophosphatase domain results in complete loss of enzymatic activity Arabidopsis thaliana
3.1.3.2 P250L mutation in metallophosphatase domain results in complete loss of enzymatic activity Arabidopsis thaliana
3.1.3.2 R274K mutation in metallophosphatase domain results in complete loss of enzymatic activity Arabidopsis thaliana
3.1.3.2 R415K mutation in metallophosphatase domain results in complete loss of enzymatic activity Arabidopsis thaliana
3.1.3.2 R455K mutation in metallophosphatase domain results in complete loss of enzymatic activity Arabidopsis thaliana

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.2 Fe Fe and Zn show a molar ratio of 1:0.75. AtPAP10 contains a bimetal nucleus composed of Fe and Zn Arabidopsis thaliana
3.1.3.2 Zn Fe and Zn show a molar ratio of 1:0.75. AtPAP10 contains a bimetal nucleus composed of Fe and Zn Arabidopsis thaliana

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.2 54200
-
 predicted from cDNA Arabidopsis thaliana
3.1.3.2 70000
-
 SDS-PAGE, GST fusion protein Arabidopsis thaliana
3.1.3.2 250000
-
 oligomer structure, using in-gel assays (electrophoresis under non-reducing conditions) Arabidopsis thaliana

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.2 Arabidopsis thaliana
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.2 affinity chromatography and gel filtration Arabidopsis thaliana

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.3.2 flower
-
 Arabidopsis thaliana
-
3.1.3.2 leaf
-
 Arabidopsis thaliana
-
3.1.3.2 root AtPAP10 is predominantly associated with the root surface after secretion rather than being released into the rhizosphere Arabidopsis thaliana
-
3.1.3.2 silique
-
 Arabidopsis thaliana
-
3.1.3.2 stem
-
 Arabidopsis thaliana
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.2 4-nitrophenyl phosphate + H2O
-
 Arabidopsis thaliana 4-nitrophenol + phosphate
-
 ?
3.1.3.2 5-bromo-4-chloro-3-indolyl phosphate + H2O
-
 Arabidopsis thaliana 5-bromo-4-chloro-3-hydroxyindole + phosphate
-
 ?
3.1.3.2 ADP + H2O
-
 Arabidopsis thaliana AMP + phosphate
-
 ?
3.1.3.2 ATP + H2O
-
 Arabidopsis thaliana ADP + phosphate
-
 ?
3.1.3.2 dATP + H2O
-
 Arabidopsis thaliana dADP + phosphate
-
 ?
3.1.3.2 diphosphate + H2O
-
 Arabidopsis thaliana phosphate
-
 ?
3.1.3.2 L-phosphoserine + H2O
-
 Arabidopsis thaliana serine + phosphate
-
 ?
3.1.3.2 polyphosphate + H2O
-
 Arabidopsis thaliana phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.2 oligomer electrophoresis under non-reducing conditions Arabidopsis thaliana

Synonyms

EC Number Synonyms Comment Organism
3.1.3.2 AtPAP10
-
 Arabidopsis thaliana
3.1.3.2 purple acid phosphatase 10
-
 Arabidopsis thaliana

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.3.2 60
-
 highest activity Arabidopsis thaliana

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.1.3.2 25 75 active between Arabidopsis thaliana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.2 5
-
 optimal activity between pH 5-6 Arabidopsis thaliana

Expression

EC Number Organism Comment Expression
3.1.3.2 Arabidopsis thaliana expression of AtPAP10 is specifically induced by phosphate limitation at both transcriptional and posttranscriptional levels up

General Information

EC Number General Information Comment Organism
3.1.3.2 malfunction functional analyses of multiple atpap10 mutant alleles and overexpressing lines show that AtPAP10 plays an important role in plant tolerance to phosphate limitation Arabidopsis thaliana
3.1.3.2 malfunction growth of Atpap10 mutants is reduced under phosphate limitation Arabidopsis thaliana