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Literature summary extracted from
- Functional identification of Arabidopsis ATSIP2 (At3g57520) as an alkaline alpha-galactosidase with a substrate specificity for raffinose and an apparent sink-specific expression pattern (2010), Plant Cell Physiol., 51, 1815-1819.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.2.1.22 | expressed in Sf9 insect cells | Arabidopsis thaliana |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.22 | 1-deoxygalactonojirimycin | - |
Arabidopsis thaliana |  |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.22 | 105 | - |
raffinose | pH 7.5, temperature not specified in the publication | Arabidopsis thaliana | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.22 | Arabidopsis thaliana | - |
- |
- |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.2.1.22 | additional information | a beta-glucuronidase reporter construct using the ATSIP2 promoter shows that ATSIP2 is strongly expressed in sink tissues of Arabidopsis, i.e. sink leaves and non-xylem parts of the root stele | Arabidopsis thaliana | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.22 | 4-nitrophenyl-alpha-D-galactopyranoside + H2O | - |
Arabidopsis thaliana | 4-nitrophenol + alpha-D-galactopyranose | - |
? | |
| 3.2.1.22 | raffinose + H2O | - |
Arabidopsis thaliana | D-galactose + sucrose | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.2.1.22 | alpha-galactosidase | - |
Arabidopsis thaliana |
| 3.2.1.22 | ATSIP2 | - |
Arabidopsis thaliana |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.22 | 7.5 | - |
- |
Arabidopsis thaliana |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.22 | 7.5 | 8 | - |
Arabidopsis thaliana |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 3.2.1.22 | physiological function | raffinose phloem unloading | Arabidopsis thaliana |
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Release 2023.1 (January 2023)
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