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Literature summary extracted from

  • Pedras, M.S.; Minic, Z.; Thongbam, P.D.; Bhaskar, V.; Montaut, S.
    Indolyl-3-acetaldoxime dehydratase from the phytopathogenic fungus Sclerotinia sclerotiorum: purification, characterization, and substrate specificity (2010), Phytochemistry, 71, 1952-1962.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.8.1.3 Na2S2O4 the specific activity of IADSs increases about 17fold upon addition of Na2S2O4 under anaerobic conditions Sclerotinia sclerotiorum

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.8.1.3 additional information no significant inhibition or activation of the enzyme occurs in the presence of dithiothreitol, FeCl3, NaN3, PLP, and FAD Sclerotinia sclerotiorum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.8.1.3 0.29
-
 (E,Z)-indolyl-3-acetaldoxime in 25 mM Tris-HCl buffer pH 7.5, at 23°C Sclerotinia sclerotiorum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.8.1.3 soluble
-
 Sclerotinia sclerotiorum
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.8.1.3 Fe2+ addition of 1 mM FeCl2 results in a slight increase of enzyme activity Sclerotinia sclerotiorum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.8.1.3 41000
-
 calculated from amino acid sequence Sclerotinia sclerotiorum
4.8.1.3 44000
-
 gel filtration Sclerotinia sclerotiorum

Organism

EC Number Organism UniProt Comment Textmining
4.8.1.3 Sclerotinia sclerotiorum A7E8M5
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.8.1.3 hydroxyapatite column chromatography, DEAE Sephacel gel filtration, and Superdex G-75 gel filtration Sclerotinia sclerotiorum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.8.1.3 mycelium
-
 Sclerotinia sclerotiorum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.8.1.3 0.0045
-
 crude extract, in 25 mM Tris-HCl buffer pH 7.5, at 23°C Sclerotinia sclerotiorum
4.8.1.3 0.175
-
 after 39fold purification, in 25 mM Tris-HCl buffer pH 7.5, at 23°C Sclerotinia sclerotiorum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.8.1.3 (E,Z)-3-(3-indolyl)propanal oxime 51% activity compared to (E,Z)-indolyl-3-acetaldoxime Sclerotinia sclerotiorum ?
-
 ?
4.8.1.3 (E,Z)-4-(3-indolyl)butanal oxime 12% activity compared to (E,Z)-indolyl-3-acetaldoxime Sclerotinia sclerotiorum ?
-
 ?
4.8.1.3 (E,Z)-4-hydroxyphenylacetaldehyde oxime 30% activity compared to (E,Z)-indolyl-3-acetaldoxime Sclerotinia sclerotiorum ?
-
 ?
4.8.1.3 (E,Z)-4-methoxyphenylacetaldoxime 19% activity compared to (E,Z)-indolyl-3-acetaldoxime Sclerotinia sclerotiorum ?
-
 ?
4.8.1.3 (E,Z)-indolyl-3-acetaldoxime 100% activity Sclerotinia sclerotiorum (E,Z)-indolyl-3-acetonitrile
-
 r
4.8.1.3 additional information the enzyme displays no activity toward (E,Z)-indole-3-carboxaldehyde oxime, (E,Z)-naphthyl-1-carboxaldehyde oxime, (E,Z)-naphthyl-2-carboxaldehyde oxime, (E,Z)-phenylacetaldoxime, (E,Z)-butanal oxime, (E,Z)-3-methylbutanal oxime, and (E,Z)-cyclopentanecarboxaldehyde oxime Sclerotinia sclerotiorum ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.8.1.3 monomer 1 * 44000, SDS-PAGE Sclerotinia sclerotiorum

Synonyms

EC Number Synonyms Comment Organism
4.8.1.3 IAD
-
 Sclerotinia sclerotiorum
4.8.1.3 indolyl-3-acetaldoxime dehydratase
-
 Sclerotinia sclerotiorum
4.8.1.3 protein SS1G-01653
-
 Sclerotinia sclerotiorum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.8.1.3 25
-
-
 Sclerotinia sclerotiorum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.8.1.3 10
-
 (E,Z)-indolyl-3-acetaldoxime in 25 mM Tris-HCl buffer pH 7.5, at 23°C Sclerotinia sclerotiorum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.8.1.3 7.2 7.5 at 25°C Sclerotinia sclerotiorum

pI Value

EC Number Organism Comment pI Value Maximum pI Value
4.8.1.3 Sclerotinia sclerotiorum calculated from amino acid sequence
-
 5.5