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Literature summary extracted from

  • Rampias, T.; Sheppard, K.; Soell, D.
    The archaeal transamidosome for RNA-dependent glutamine biosynthesis (2010), Nucleic Acids Res., 38, 5774-5783.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.3.5.7 expressed in Escherichia coli as a His-tagged fusion protein Methanothermobacter thermautotrophicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.1.1.24 GatDE a heterodimeric amidotransferase. In the presence of GatDE at 1.8 mM, the Glu-tRNAGlu activity of ND-GluRS decreases almost in half Methanothermobacter thermautotrophicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.5.7 0.0017
-
 Glu-tRNAGln 37°C, pH not specified in the publication Methanothermobacter thermautotrophicus
6.3.5.7 0.0023
-
 Glu-tRNAGln 37°C, pH not specified in the publication, in the presence of glutamyl-tRNA synthetase (0.002 mM) Methanothermobacter thermautotrophicus
6.3.5.7 0.0024
-
 Glu-tRNAGln 37°C, pH not specified in the publication, in the presence of bovine serum albumin (0.002 mM) Methanothermobacter thermautotrophicus

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.24 Methanothermobacter thermautotrophicus
-
-
-
6.3.5.7 Methanothermobacter thermautotrophicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.1.1.24 native ND-GluRS by gel filtration Methanothermobacter thermautotrophicus
6.3.5.7 using Ni-NTA chromatography Methanothermobacter thermautotrophicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.5.7 ATP + Glu-tRNAGln + L-glutamine
-
 Methanothermobacter thermautotrophicus ADP + phosphate + Gln-tRNAGln + L-glutamate
-
 ?
6.3.5.7 additional information the association of archaeal glutamyl-tRNA synthetase (ND-GluRS) with GatDE sequesters the tRNA synthetase for Gln-tRNAGln formation, with GatDE reducing the affinity of glutamyl-tRNA synthetase (ND-GluRS) for tRNAGlu 13fold Methanothermobacter thermautotrophicus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
6.1.1.24 More ND-GluRS:GatDE complex formation and structure, detailed overview Methanothermobacter thermautotrophicus
6.3.5.7 homodimer gel filtration, GatDE forms a homodimer with the binding interface between the D-subunits Methanothermobacter thermautotrophicus

Synonyms

EC Number Synonyms Comment Organism
6.1.1.24 ND-GluRS
-
 Methanothermobacter thermautotrophicus
6.1.1.24 non-discriminating glutamyl-tRNA synthetase
-
 Methanothermobacter thermautotrophicus
6.3.5.7 GatDE
-
 Methanothermobacter thermautotrophicus
6.3.5.7 Glu-amidotransferase
-
 Methanothermobacter thermautotrophicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.1.1.24 37
-
 assay at Methanothermobacter thermautotrophicus
6.3.5.7 37
-
 assay at Methanothermobacter thermautotrophicus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.3.5.7 0.9
-
 Glu-tRNAGln 37°C, pH not specified in the publication Methanothermobacter thermautotrophicus
6.3.5.7 0.9
-
 Glu-tRNAGln 37°C, pH not specified in the publication, in the presence of bovine serum albumin (0.002 mM) Methanothermobacter thermautotrophicus
6.3.5.7 1.2
-
 Glu-tRNAGln 37°C, pH not specified in the publication, in the presence of glutamyl-tRNA synthetase (0.002 mM) Methanothermobacter thermautotrophicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.1.1.24 7.2 7.5 assay at Methanothermobacter thermautotrophicus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
6.1.1.24 additional information
-
 GatDE apparent KI of GatDE for ND-GluRS in the Glu-tRNAGlu reaction is 31 nM Methanothermobacter thermautotrophicus

General Information

EC Number General Information Comment Organism
6.1.1.24 evolution ND-GluRS is a class I aminoacyl-tRNA synthetase Methanothermobacter thermautotrophicus
6.1.1.24 additional information ND-GluRS:GatDE complex formation and structure, detailed overview Methanothermobacter thermautotrophicus
6.1.1.24 physiological function for synthesis of Gln-tRNAGln, a two-step process is required: the non-discriminating glutamyl-tRNA synthetase, ND-GluRS, forms Glu-tRNAGln, while the heterodimeric amidotransferase GatDE converts this mischarged tRNA to Gln-tRNAGln. A similar complex for Gln-tRNAGln formation in Methanothermobacter thermautotrophicus that allows the mischarged Glu-tRNAGln made by the tRNA synthetase to be channeled to the amidotransferase. The association of archaeal ND-GluRS with GatDE sequesters the tRNA synthetase for Gln-tRNAGln formation, with GatDE reducing the affinity of ND-GluRS for tRNAGlu by at least 13fold. The archaeal complex does not require tRNA for its formation, is not stable through product (Gln-tRNAGln) formation, and has no major effect on the kinetics of tRNAGln glutamylation nor transamidation Methanothermobacter thermautotrophicus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
6.3.5.7 400
-
 Glu-tRNAGln 37°C, pH not specified in the publication, in the presence of bovine serum albumin (0.002 mM) Methanothermobacter thermautotrophicus
6.3.5.7 500
-
 Glu-tRNAGln 37°C, pH not specified in the publication Methanothermobacter thermautotrophicus
6.3.5.7 600
-
 Glu-tRNAGln 37°C, pH not specified in the publication, in the presence of glutamyl-tRNA synthetase (0.002 mM) Methanothermobacter thermautotrophicus