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Literature summary extracted from

  • van den Born, E.; Omelchenko, M.V.; Bekkelund, A.; Leihne, V.; Koonin, E.V.; Dolja, V.V.; Falnes, P.?.
    Viral AlkB proteins repair RNA damage by oxidative demethylation (2008), Nucleic Acids Res., 36, 5451-5461.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.11.33 expressed in Escherichia coli BL21-CodonPlus(DE3)-RIPL cells blueberry scorch virus
1.14.11.33 expressed in Escherichia coli BL21-CodonPlus(DE3)-RIPL cells Blackberry virus Y
1.14.11.33 expressed in Escherichia coli BL21-CodonPlus(DE3)-RIPL cells grapevine virus A

Protein Variants

EC Number Protein Variants Comment Organism
1.14.11.33 D61A the mutant is unable to rescue methylated RNA phage MS2 and is unable to convert 2-oxoglutarate to succinate Blackberry virus Y
1.14.11.33 H59A the mutant is unable to rescue methylated RNA phage MS2 and is unable to convert 2-oxoglutarate to succinate Blackberry virus Y

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.11.33 Fe2+ required for activity Escherichia coli
1.14.11.33 Fe2+ required for activity blueberry scorch virus
1.14.11.33 Fe2+ required for activity Blackberry virus Y
1.14.11.33 Fe2+ required for activity grapevine virus A

Organism

EC Number Organism UniProt Comment Textmining
1.14.11.33 Blackberry virus Y
-
-
-
1.14.11.33 blueberry scorch virus
-
-
-
1.14.11.33 Escherichia coli
-
-
-
1.14.11.33 grapevine virus A
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.11.33 TALON metal affinity resin column chromatography blueberry scorch virus
1.14.11.33 TALON metal affinity resin column chromatography Blackberry virus Y
1.14.11.33 TALON metal affinity resin column chromatography grapevine virus A

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.11.33 DNA-1-methyladenine + 2-oxoglutarate + O2
-
 Escherichia coli DNA-adenine + formaldehyde + succinate + CO2
-
 ?
1.14.11.33 DNA-1-methyladenine + 2-oxoglutarate + O2
-
 blueberry scorch virus DNA-adenine + formaldehyde + succinate + CO2
-
 ?
1.14.11.33 DNA-1-methyladenine + 2-oxoglutarate + O2
-
 Blackberry virus Y DNA-adenine + formaldehyde + succinate + CO2
-
 ?
1.14.11.33 DNA-1-methyladenine + 2-oxoglutarate + O2
-
 grapevine virus A DNA-adenine + formaldehyde + succinate + CO2
-
 ?
1.14.11.33 DNA-3-methylcytosine + 2-oxoglutarate + O2
-
 Escherichia coli DNA-cytosine + formaldehyde + succinate + CO2
-
 ?
1.14.11.33 DNA-3-methylcytosine + 2-oxoglutarate + O2
-
 blueberry scorch virus DNA-cytosine + formaldehyde + succinate + CO2
-
 ?
1.14.11.33 DNA-3-methylcytosine + 2-oxoglutarate + O2
-
 Blackberry virus Y DNA-cytosine + formaldehyde + succinate + CO2
-
 ?
1.14.11.33 DNA-3-methylcytosine + 2-oxoglutarate + O2
-
 grapevine virus A DNA-cytosine + formaldehyde + succinate + CO2
-
 ?
1.14.11.33 methylated DNA bacteriophage M13mp18 + 2-oxoglutarate + O2
-
 blueberry scorch virus DNA bacteriophage M13mp18 + formaldehyde + succinate + CO2
-
 ?
1.14.11.33 methylated DNA bacteriophage M13mp18 + 2-oxoglutarate + O2
-
 Blackberry virus Y DNA bacteriophage M13mp18 + formaldehyde + succinate + CO2
-
 ?
1.14.11.33 methylated DNA bacteriophage M13mp18 + 2-oxoglutarate + O2
-
 grapevine virus A DNA bacteriophage M13mp18 + formaldehyde + succinate + CO2
-
 ?
1.14.11.33 methylated RNA bacteriophage MS2 + 2-oxoglutarate + O2
-
 blueberry scorch virus RNA bacteriophage MS2 + formaldehyde + succinate + CO2
-
 ?
1.14.11.33 methylated RNA bacteriophage MS2 + 2-oxoglutarate + O2
-
 Blackberry virus Y RNA bacteriophage MS2 + formaldehyde + succinate + CO2
-
 ?
1.14.11.33 methylated RNA bacteriophage MS2 + 2-oxoglutarate + O2
-
 grapevine virus A RNA bacteriophage MS2 + formaldehyde + succinate + CO2
-
 ?
1.14.11.33 additional information Escherichia coli AlkB is approximately 10fold more active on single-stranded DNA compared to single-stranded RNA, Eschichia coli AlkB displays a somewhat lower activity on double-stranded RNA substrates than on single-stranded RNA substrates Escherichia coli ?
-
 ?
1.14.11.33 additional information the viral AlkB protein prefers single-stranded RNA over single-stranded DNA substrates (10fold) blueberry scorch virus ?
-
 ?
1.14.11.33 additional information the viral AlkB protein prefers single-stranded RNA over single-stranded DNA substrates (10fold) Blackberry virus Y ?
-
 ?
1.14.11.33 additional information the viral AlkB protein prefers single-stranded RNA over single-stranded DNA substrates (10fold) grapevine virus A ?
-
 ?
1.14.11.33 RNA-1-methyladenine + 2-oxoglutarate + O2
-
 Escherichia coli RNA-adenine + formaldehyde + succinate + CO2
-
 ?
1.14.11.33 RNA-1-methyladenine + 2-oxoglutarate + O2
-
 blueberry scorch virus RNA-adenine + formaldehyde + succinate + CO2
-
 ?
1.14.11.33 RNA-1-methyladenine + 2-oxoglutarate + O2
-
 Blackberry virus Y RNA-adenine + formaldehyde + succinate + CO2
-
 ?
1.14.11.33 RNA-1-methyladenine + 2-oxoglutarate + O2
-
 grapevine virus A RNA-adenine + formaldehyde + succinate + CO2
-
 ?
1.14.11.33 RNA-3-methylcytosine + 2-oxoglutarate + O2
-
 Escherichia coli RNA-cytosine + formaldehyde + succinate + CO2
-
 ?
1.14.11.33 RNA-3-methylcytosine + 2-oxoglutarate + O2
-
 blueberry scorch virus RNA-cytosine + formaldehyde + succinate + CO2
-
 ?
1.14.11.33 RNA-3-methylcytosine + 2-oxoglutarate + O2
-
 Blackberry virus Y RNA-cytosine + formaldehyde + succinate + CO2
-
 ?
1.14.11.33 RNA-3-methylcytosine + 2-oxoglutarate + O2
-
 grapevine virus A RNA-cytosine + formaldehyde + succinate + CO2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.11.33 AlkB
-
 Escherichia coli
1.14.11.33 AlkB
-
 blueberry scorch virus
1.14.11.33 AlkB
-
 Blackberry virus Y
1.14.11.33 AlkB
-
 grapevine virus A

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.11.33 2-oxoglutarate
-
 Escherichia coli
1.14.11.33 2-oxoglutarate
-
 blueberry scorch virus
1.14.11.33 2-oxoglutarate
-
 Blackberry virus Y
1.14.11.33 2-oxoglutarate
-
 grapevine virus A

General Information

EC Number General Information Comment Organism
1.14.11.33 physiological function viral AlkB proteins maintain the integrity of the viral RNA genome through repair of deleterious methylation damage blueberry scorch virus
1.14.11.33 physiological function viral AlkB proteins maintain the integrity of the viral RNA genome through repair of deleterious methylation damage Blackberry virus Y
1.14.11.33 physiological function viral AlkB proteins maintain the integrity of the viral RNA genome through repair of deleterious methylation damage grapevine virus A