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Literature summary extracted from

  • Slade, D.; Dunstan, M.; Barkauskaite, E.; Weston, R.; Lafite, P.; Dixon, N.; Ahel, M.; Leys, D.; Ahel, I.
    The structure and catalytic mechanism of a poly(ADP-ribose) glycohydrolase (2011), Nature, 477, 616-622.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.143 expressed in Escherichia coli Homo sapiens
3.2.1.143 expressed in Escherichia coli Thermomonospora curvata

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.2.1.143 in complexes with ADP-ribose and inhibitor adenosine 5'-diphosphate (hydroxymethyl) pyrrolidinediol Thermomonospora curvata

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.143 A874W the mutant shows about 45% activity compared to the wild type enzyme Homo sapiens
3.2.1.143 E114A the mutant shows about 2% activity compared to the wild type enzyme Thermomonospora curvata
3.2.1.143 E115A inactive Thermomonospora curvata
3.2.1.143 E755A inactive Homo sapiens
3.2.1.143 E756A inactive Homo sapiens
3.2.1.143 F227A inactive Thermomonospora curvata
3.2.1.143 F875A the mutant shows about 1% activity compared to the wild type enzyme Homo sapiens
3.2.1.143 N740A the mutant shows about 30% activity compared to the wild type enzyme Homo sapiens
3.2.1.143 S298K the mutant shows about 2% activity compared to the wild type enzyme Thermomonospora curvata
3.2.1.143 V226W the mutant shows about 5% activity compared to the wild type enzyme Thermomonospora curvata

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.143 Homo sapiens
-
-
-
3.2.1.143 Thermomonospora curvata
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.143 poly(ADP-ribose) + H2O
-
 Homo sapiens ADP-ribose oligomer + ADP-ribose
-
 ?
3.2.1.143 poly(ADP-ribose) + H2O
-
 Thermomonospora curvata ADP-ribose oligomer + ADP-ribose
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.143 PARG
-
 Homo sapiens
3.2.1.143 PARG
-
 Thermomonospora curvata