Literature summary extracted from

Yi, C.; Jia, G.; Hou, G.; Dai, Q.; Zhang, W.; Zheng, G.; Jian, X.; Yang, C.G.; Cui, Q.; He, C.
Iron-catalysed oxidation intermediates captured in a DNA repair dioxygenase (2010), Nature, 468, 330-333.

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
1.14.11.33	expressed in Escherichia coli BL21(DE3) cells	Escherichia coli

Crystallization (Commentary)

EC Number	Crystallization (Comment)	Organism
1.14.11.33	AlkB in complex with double-stranded DNA containing 1,N6-ethenoadenine, 3-methylthymine, and 3-methylcytosine, using 100 mM NaCl, 25 mM MgCl2, 100 mM cacodylate (pH 6.5) and 20-24% (w/v) PEG 8K or 4K	Escherichia coli

Protein Variants

EC Number	Protein Variants	Comment	Organism
1.14.11.33	D135A	the mutant displays about 5-15% of wild type activity towards 1-methyladenine at pH 7.0	Escherichia coli
1.14.11.33	D135N	the mutant displays about 5-15% of wild type activity towards 1-methyladenine at pH 7.0	Escherichia coli
1.14.11.33	D135S	the mutant displays about 5-15% of wild type activity towards 1-methyladenine at pH 7.0	Escherichia coli

Metals/Ions

EC Number	Metals/Ions	Comment	Organism	Structure
1.14.11.33	Fe2+	required for activity	Escherichia coli

Organism

EC Number	Organism	UniProt	Comment	Textmining
1.14.11.33	Escherichia coli	P05050	-	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
1.14.11.33	Ni-NTA column chromatography and Mono-Q column chromatography	Escherichia coli

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
1.14.11.33	DNA-1,N6-ethenoadenine + 2-oxoglutarate + O2	-	Escherichia coli	?	-	?
1.14.11.33	DNA-1-methyladenine + 2-oxoglutarate + O2	-	Escherichia coli	DNA-adenine + formaldehyde + succinate + CO2	-	?
1.14.11.33	DNA-3-methylcytosine + 2-oxoglutarate + O2	preferred substrate of AlkB	Escherichia coli	DNA-cytosine + formaldehyde + succinate + CO2	-	?
1.14.11.33	DNA-3-methylthymine + 2-oxoglutarate + O2	-	Escherichia coli	DNA-thymine + formaldehyde + succinate + CO2	-	?

Synonyms

EC Number	Synonyms	Comment	Organism
1.14.11.33	AlkB	-	Escherichia coli

Cofactor

EC Number	Cofactor	Comment	Organism	Structure
1.14.11.33	2-oxoglutarate	-	Escherichia coli

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Release 2023.1 (January 2023)