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Literature summary extracted from

  • Hemmert, A.C.; Otto, T.C.; Wierdl, M.; Edwards, C.C.; Fleming, C.D.; MacDonald, M.; Cashman, J.R.; Potter, P.M.; Cerasoli, D.M.; Redinbo, M.R.
    Human carboxylesterase 1 stereoselectively binds the nerve agent cyclosarin and spontaneously hydrolyzes the nerve agent sarin (2010), Mol. Pharmacol., 77, 508-516.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.1.1 sarin for enzyme inhibited by racemic mixtures of bona fide nerve agents, hCE1 spontaneously reactivates in the presence of sarin but not soman or cyclosarin. The addition of 2,3-butanedione monoxime increases the rate of reactivation of CE1 from sarin inhibition by more than 65fold Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.1 expressed in baculovirus-infected Spodoptera frugiperda Sf21 cells Homo sapiens

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.1.1.1 carboxylesterase 1 in complex with cyclosarin, sitting drop vapor diffusion method, using 10% (w/v) polyethylene glycol 3350, 0.3 M Li2SO4, 0.1 M citrate, pH 5.5, 0.1 M NaCl, 0.1 M LiCl, and 5% (v/v) glycerol. Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.1 cyclosarin
-
 Homo sapiens
3.1.1.1 additional information 2,3-butanedione monoxime has no inhibitory effect on CE1 Homo sapiens
3.1.1.1 soman
-
 Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.1 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.1 gel filtration Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.1 4-nitrophenyl butyrate + H2O
-
 Homo sapiens 4-nitrophenol + butyrate
-
 ?
3.1.1.1 additional information human carboxylesterase 1 stereoselectively binds the nerve agent cyclosarin and exhibits a 1700- and 2900fold preference for the PR enantiomers of analogs of soman and cyclosarin, respectively Homo sapiens ?
-
 ?
3.1.1.1 sarin + H2O CE1 exhibits a 5fold preference for the PS isomer of a sarin analog Homo sapiens ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.1.1 carboxylesterase 1
-
 Homo sapiens
3.1.1.1 CE1
-
 Homo sapiens