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Literature summary extracted from

  • Cardona-Felix, C.S.; Pastor-Palacios, G.; Cardenas, H.; Azuara-Liceaga, E.; Brieba, L.G.
    Biochemical characterization of the DNA ligase I from Entamoeba histolytica (2010), Mol. Biochem. Parasitol., 174, 26-35.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.5.1.1 expressed in Escherichia coli BL21-Start cells Entamoeba histolytica

Protein Variants

EC Number Protein Variants Comment Organism
6.5.1.1 K326 catalytically inactive Entamoeba histolytica

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.5.1.1 NaCl complete inhibition at above 200 mM Entamoeba histolytica

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.5.1.1 0.000064
-
 ATP in 50 mM Tris pH 7.5, 100 mM NaCl, 10 mM dithiothreitol, at 21°C Entamoeba histolytica

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
6.5.1.1 cytoplasm
-
 Entamoeba histolytica 5737
-
6.5.1.1 nucleus a population of the DNAligI protein is translocated from the cytoplasm into the nuclei Entamoeba histolytica 5634
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.5.1.1 Mg2+ DNAligI uses Mn2+ or Mg2+ as metal cofactors, optimal ligation activity at 4 mM of Mg2+ Entamoeba histolytica
6.5.1.1 Mn2+ DNAligI uses Mn2+ or Mg2+ as metal cofactors, optimal ligation activity at 1 mM of Mn2+ Entamoeba histolytica

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.5.1.1 75000
-
 x * 75000, SDS-PAGE Entamoeba histolytica

Organism

EC Number Organism UniProt Comment Textmining
6.5.1.1 Entamoeba histolytica C4M5H3
-
-
6.5.1.1 Entamoeba histolytica HM1:IMSS C4M5H3
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.5.1.1 HiTrap column chromatography and phosphocellulose column chromatography Entamoeba histolytica

Source Tissue

EC Number Source Tissue Comment Organism Textmining
6.5.1.1 trophozoite
-
 Entamoeba histolytica
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.5.1.1 ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m
-
 Entamoeba histolytica AMP + diphosphate + (deoxyribonucleotide)m+n
-
 ?
6.5.1.1 ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m
-
 Entamoeba histolytica HM1:IMSS AMP + diphosphate + (deoxyribonucleotide)m+n
-
 ?
6.5.1.1 additional information DNAligI performs the three conserved steps of a DNA ligation reaction: adenylation, binding to a 5'-phosphorylated nicked DNA substrate and sealing of the nick. DNAligI is also able to ligate a RNA strand upstream of a nucleic acid nick, but not in the downstream or the template position Entamoeba histolytica ?
-
 ?
6.5.1.1 additional information DNAligI performs the three conserved steps of a DNA ligation reaction: adenylation, binding to a 5'-phosphorylated nicked DNA substrate and sealing of the nick. DNAligI is also able to ligate a RNA strand upstream of a nucleic acid nick, but not in the downstream or the template position Entamoeba histolytica HM1:IMSS ?
-
 ?

Subunits

EC Number Subunits Comment Organism
6.5.1.1 ? x * 75000, SDS-PAGE Entamoeba histolytica

Synonyms

EC Number Synonyms Comment Organism
6.5.1.1 DNA ligase I
-
 Entamoeba histolytica
6.5.1.1 DNAligI
-
 Entamoeba histolytica

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.5.1.1 37
-
-
 Entamoeba histolytica

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.5.1.1 0.04
-
 ATP in 50 mM Tris pH 7.5, 100 mM NaCl, 10 mM dithiothreitol, at 21°C Entamoeba histolytica

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5.1.1 7.5
-
-
 Entamoeba histolytica

pH Range

EC Number pH Minimum pH Maximum Comment Organism
6.5.1.1 6.5 8.5 DNAligI activity gradually increases from pH 6.5 to 8.0 and displays a sharp decline at pH 8.5 Entamoeba histolytica

Cofactor

EC Number Cofactor Comment Organism Structure
6.5.1.1 ATP specifically dependent on ATP Entamoeba histolytica
6.5.1.1 additional information DNAligI is not active using NAD+, UTP, CTP, GTP, dATP, and dGTP as cofactors Entamoeba histolytica

General Information

EC Number General Information Comment Organism
6.5.1.1 physiological function DNAligI is involved in sealing DNA nicks during lagging strand synthesis and may have a role in base excision repair in Entamoeba histolytica Entamoeba histolytica

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
6.5.1.1 625
-
 ATP in 50 mM Tris pH 7.5, 100 mM NaCl, 10 mM dithiothreitol, at 21°C Entamoeba histolytica