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Literature summary extracted from

  • Chan, S.I.; Nguyen, H.H.; Chen, K.H.; Yu, S.S.
    Overexpression and purification of the particulate methane monooxygenase from Methylococcus capsulatus (Bath) (2011), Methods Enzymol., 495, 177-193.
    View publication on PubMed

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.18.3 membrane
-
 Methylococcus capsulatus 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.18.3 Cu2+ multi-copper enzyme with 14.1 copper atoms per protein, highest specific activity is observed wit 0.04 mM Cu2+ in the growth medium Methylococcus capsulatus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.18.3 23000
-
 1 * 45000 + 1 * 27000 + 1 * 23000, SDS-PAGE Methylococcus capsulatus
1.14.18.3 27000
-
 1 * 45000 + 1 * 27000 + 1 * 23000, SDS-PAGE Methylococcus capsulatus
1.14.18.3 28328
-
 1 * 42785 + 1 * 29073 + 1 * 28328, MALDI-TOF mass spectrometry Methylococcus capsulatus
1.14.18.3 28376
-
 1 * 42786 + 1 * 29063 + 1 * 28376, calculated from amino acid sequence Methylococcus capsulatus
1.14.18.3 29063
-
 1 * 42786 + 1 * 29063 + 1 * 28376, calculated from amino acid sequence Methylococcus capsulatus
1.14.18.3 29073
-
 1 * 42785 + 1 * 29073 + 1 * 28328, MALDI-TOF mass spectrometry Methylococcus capsulatus
1.14.18.3 42785
-
 1 * 42785 + 1 * 29073 + 1 * 28328, MALDI-TOF mass spectrometry Methylococcus capsulatus
1.14.18.3 42786
-
 1 * 42786 + 1 * 29063 + 1 * 28376, calculated from amino acid sequence Methylococcus capsulatus
1.14.18.3 45000
-
 1 * 45000 + 1 * 27000 + 1 * 23000, SDS-PAGE Methylococcus capsulatus
1.14.18.3 100000
-
 apo-protein Methylococcus capsulatus
1.14.18.3 220000
-
 purified pMMO-detergent complex, gel filtration Methylococcus capsulatus

Organism

EC Number Organism UniProt Comment Textmining
1.14.18.3 Methylococcus capsulatus
-
-
-
1.14.18.3 Methylococcus capsulatus ATCC 33009
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.18.3 DEAE-Sepharose Fast Flow column chromatography, lysine agarose column chromatography, Sephacryl S-300HR gel filtration, QEA-Sephadex A-50 column chromatography, and Sephacryl S200 gel filtration, Superdex 200 gel filtration, or ammonium sulfate precipitation followed by Source 30Q column chroamtography Methylococcus capsulatus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.18.3 propylene + duroquinol + O2
-
 Methylococcus capsulatus propylene oxide + duroquinone + H2O
-
 ?
1.14.18.3 propylene + duroquinol + O2
-
 Methylococcus capsulatus ATCC 33009 propylene oxide + duroquinone + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.18.3 heterotrimer 1 * 42785 + 1 * 29073 + 1 * 28328, MALDI-TOF mass spectrometry Methylococcus capsulatus
1.14.18.3 heterotrimer 1 * 42786 + 1 * 29063 + 1 * 28376, calculated from amino acid sequence Methylococcus capsulatus
1.14.18.3 heterotrimer 1 * 45000 + 1 * 27000 + 1 * 23000, SDS-PAGE Methylococcus capsulatus

Synonyms

EC Number Synonyms Comment Organism
1.14.18.3 particulate methane monooxygenase
-
 Methylococcus capsulatus
1.14.18.3 pMMO
-
 Methylococcus capsulatus