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Literature summary extracted from

  • Peebles, C.A.; Sander, G.W.; Hughes, E.H.; Peacock, R.; Shanks, J.V.; San, K.Y.
    The expression of 1-deoxy-d-xylulose synthase and geraniol-10-hydroxylase or anthranilate synthase increases terpenoid indole alkaloid accumulation in Catharanthus roseus hairy roots (2010), Metab. Eng., 13, 234-240.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.14.83 overexpression in Catharanthus roseus hairy roots via Agrobacterium rhizogenes 15834 transfection system, co-expression with the 1-deoxy-D-xylulose synthase, DXS, from Arabidopsis thaliana Catharanthus roseus
4.1.3.27 alpha subunit (with a point mutation to confer feedback resistance to tryptophan) expressed in hairy root of Catharanthus roseus Arabidopsis thaliana

Protein Variants

EC Number Protein Variants Comment Organism
1.14.14.83 additional information co-overexpression of G10H with the 1-deoxy-D-xylulose synthase, DXS, from Arabidopsis thaliana causes a significant increase in ajmalicine by 16%, lochnericine by 31% and tabersonine by 13%. To increase the flux toward vinblastine and vincristine, a multiple genes within the pathway need to be overexpressed, terpenoid indole alkaloid profile, overview Catharanthus roseus
1.14.14.83 additional information construction of hairy root lines with inducible overexpression of 1-deoxy-D-xylulose synthase, DXS, or geraniol-10-hydroxylase, G10H Catharanthus roseus

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.83 Catharanthus roseus
-
-
-
4.1.3.27 Arabidopsis thaliana
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.14.83 hairy root
-
 Catharanthus roseus
-
4.1.3.27 hairy root
-
 Arabidopsis thaliana
-

Synonyms

EC Number Synonyms Comment Organism
1.14.14.83 G10H
-
 Catharanthus roseus
1.14.14.83 geraniol-10-hydroxylase
-
 Catharanthus roseus
4.1.3.27 anthranilate synthase
-
 Arabidopsis thaliana

General Information

EC Number General Information Comment Organism
1.14.14.83 metabolism the enzyme is part of the terpenoid indole alkaloid pathway Catharanthus roseus
1.14.14.83 additional information the individual overexpression of the terpenoid genes 1-deoxy-D-xylulose synthase, DXS, and G10H resulted in mixed results in regards to the accumulation of terpenoid indole alkaloid metabolite pools Catharanthus roseus