Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Mora-Montes, H.M.; Robledo-Ortiz, C.I.; Gonzalez-Sanchez, L.C.; Lopez-Esparza, A.; Lopez-Romero, E.; Flores-Carreon, A.
    Purification and biochemical characterisation of endoplasmic reticulum alpha1,2-mannosidase from Sporothrix schenckii (2010), Mem. Inst. Oswaldo Cruz, 105, 79-85.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.209 1-deoxymannojirimycin the enzyme is inhibited preferentially by 1-deoxymannojirimycin Sporothrix schenckii
3.2.1.209 Co2+ 50% reduction in alpha-mannosidase activity is observed after addition of 3 mM CoCl2 Sporothrix schenckii
3.2.1.209 additional information not affected by EDTA Sporothrix schenckii
3.2.1.209 swainsonine
-
 Sporothrix schenckii
3.2.1.209 Zn2+ 50% reduction in alpha-mannosidase activity is observed after addition of 3 mM ZnCl2 Sporothrix schenckii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.209 0.04
-
 4-methylumbelliferyl alpha-D-mannopyranoside at pH 7.0 in 50 mM MES-Tris buffer and at 37°C Sporothrix schenckii

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.209 endoplasmic reticulum with the catalytic domain within the lumen of this compartment Sporothrix schenckii 5783
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.209 additional information the enzyme activity os not sensitive to the presence of Ca2+, Mg2+ and Mn2+ at concentrations up to 3 mM Sporothrix schenckii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.209 75000
-
 x * 75000, SDS-PAGE Sporothrix schenckii

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.209 Sporothrix schenckii
-
-
-
3.2.1.209 Sporothrix schenckii EH206
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.209 DEAE Bio-Gel A column chromatography Sporothrix schenckii

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.209 4-methylumbelliferyl alpha-D-mannopyranoside + H2O
-
 Sporothrix schenckii 4-methylumbelliferone + alpha-D-mannopyranoside
-
 ?
3.2.1.209 4-methylumbelliferyl alpha-D-mannopyranoside + H2O
-
 Sporothrix schenckii EH206 4-methylumbelliferone + alpha-D-mannopyranoside
-
 ?
3.2.1.209 4-nitrophenyl alpha-D-mannopyranoside + H2O
-
 Sporothrix schenckii 4-nitrophenol + alpha-D-mannopyranoside
-
 ?
3.2.1.209 4-nitrophenyl alpha-D-mannopyranoside + H2O
-
 Sporothrix schenckii EH206 4-nitrophenol + alpha-D-mannopyranoside
-
 ?
3.2.1.209 Manalpha(1->2)Manalpha(1->2)Manalpha(1->3)[Manalpha(1->2)Manalpha(1->6)[Manalpha(1->2)Manalpha(1->3)]Manalpha(1->6)]Manbeta(1->4)GlcNAcbeta(1->4)GlcNAcbeta-2-pyridylamine + H2O
-
 Sporothrix schenckii Manalpha(1->2)Manalpha(1->3)[Manalpha(1->2)Manalpha(1->6)[Manalpha(1->2)Manalpha(1->3)]Manalpha(1->6)]Manbeta(1->4)GlcNAcbeta(1->4)GlcNAcbeta-2-pyridylamine + D-mannose
-
 ?
3.2.1.209 Manalpha(1->2)Manalpha(1->2)Manalpha(1->3)[Manalpha(1->2)Manalpha(1->6)[Manalpha(1->2)Manalpha(1->3)]Manalpha(1->6)]Manbeta(1->4)GlcNAcbeta(1->4)GlcNAcbeta-2-pyridylamine + H2O
-
 Sporothrix schenckii EH206 Manalpha(1->2)Manalpha(1->3)[Manalpha(1->2)Manalpha(1->6)[Manalpha(1->2)Manalpha(1->3)]Manalpha(1->6)]Manbeta(1->4)GlcNAcbeta(1->4)GlcNAcbeta-2-pyridylamine + D-mannose
-
 ?
3.2.1.209 Manalpha(1->3)[Manalpha(1->6)[Manalpha(1->2)Manalpha(1->3)]Manalpha(1->6)]Manbeta(1->4)GlcNAcbeta(1->4)GlcNAcbeta-Asn + H2O
-
 Sporothrix schenckii Manalpha(1->3)[Manalpha(1->6)[Manalpha(1->3)]Manalpha(1->6)]Manbeta(1->4)GlcNAcbeta(1->4)GlcNAcbeta + D-mannose
-
 ?
3.2.1.209 Manalpha(1->3)[Manalpha(1->6)[Manalpha(1->2)Manalpha(1->3)]Manalpha(1->6)]Manbeta(1->4)GlcNAcbeta(1->4)GlcNAcbeta-Asn + H2O
-
 Sporothrix schenckii EH206 Manalpha(1->3)[Manalpha(1->6)[Manalpha(1->3)]Manalpha(1->6)]Manbeta(1->4)GlcNAcbeta(1->4)GlcNAcbeta + D-mannose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.209 ? x * 75000, SDS-PAGE Sporothrix schenckii

Synonyms

EC Number Synonyms Comment Organism
3.2.1.209 endoplasmic reticulum alpha1,2-mannosidase
-
 Sporothrix schenckii

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
3.2.1.209 0.12
-
 at pH 7.0 in 50 mM MES-Tris buffer and at 37°C Sporothrix schenckii 1-deoxymannojirimycin
3.2.1.209 0.56
-
 at pH 7.0 in 50 mM MES-Tris buffer and at 37°C Sporothrix schenckii swainsonine