Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Cheng, L.; Mu, W.; Jiang, B.
    Thermostable L-arabinose isomerase from Bacillus stearothermophilus IAM 11001 for D-tagatose production: gene cloning, purification and characterisation (2010), J. Sci. Food Agric., 90, 1327-1333.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.3.1.4 expressed in Escherichia coli BL21(DE3) cells Geobacillus stearothermophilus

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.3.1.4 Ba2+ 6% residual activity at 1 mM Geobacillus stearothermophilus
5.3.1.4 Ca2+ complete inhibition at 1 mM Geobacillus stearothermophilus
5.3.1.4 Co2+ 90% residual activity at 1 mM Geobacillus stearothermophilus
5.3.1.4 Cu2+ 53% residual activity at 1 mM Geobacillus stearothermophilus
5.3.1.4 EDTA 22% residual activity at 1 mM Geobacillus stearothermophilus
5.3.1.4 Mg2+ 90% residual activity at 1 mM Geobacillus stearothermophilus
5.3.1.4 Zn2+ 61% residual activity at 1 mM Geobacillus stearothermophilus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
5.3.1.4 Fe2+ 106% relative activity at 1 mM Geobacillus stearothermophilus
5.3.1.4 Mn2+ 404% relative activity at 1 mM, the enzyme has an absolute requirement for the divalent metal ion Mn2+ for both catalytic activity and thermostability Geobacillus stearothermophilus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.3.1.4 56000
-
 x * 56000, enzyme with C-terminal His-tag, SDS-PAGE Geobacillus stearothermophilus

Organism

EC Number Organism UniProt Comment Textmining
5.3.1.4 Geobacillus stearothermophilus
-
-
-
5.3.1.4 Geobacillus stearothermophilus IAM 11001
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.3.1.4 Ni-Sepharose column chromatography, gel filtration Geobacillus stearothermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.3.1.4 D-galactose the bioconversion yield of D-galactose to D-tagatose by the purified enzyme after 12 h at 65°C reaches 36% Geobacillus stearothermophilus D-tagatose
-
 ?
5.3.1.4 D-galactose the bioconversion yield of D-galactose to D-tagatose by the purified enzyme after 12 h at 65°C reaches 36% Geobacillus stearothermophilus IAM 11001 D-tagatose
-
 ?
5.3.1.4 L-arabinose
-
 Geobacillus stearothermophilus L-ribulose
-
 ?
5.3.1.4 L-arabinose
-
 Geobacillus stearothermophilus IAM 11001 L-ribulose
-
 ?

Subunits

EC Number Subunits Comment Organism
5.3.1.4 ? x * 56000, enzyme with C-terminal His-tag, SDS-PAGE Geobacillus stearothermophilus

Synonyms

EC Number Synonyms Comment Organism
5.3.1.4 L-AI
-
 Geobacillus stearothermophilus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5.3.1.4 65
-
-
 Geobacillus stearothermophilus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
5.3.1.4 45 70
-
 Geobacillus stearothermophilus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.3.1.4 7.5
-
-
 Geobacillus stearothermophilus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
5.3.1.4 5 8.5 the enzyme exhibits 82% and 65% of its maximum activity at pH 6.0 and 5.0 respectively and maintains more than 80% activity at pH values ranging from 6.0 to 8.5 Geobacillus stearothermophilus