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Literature summary extracted from

  • Thoern, C.; Gustafsson, H.; Olsson, L.
    Immobilization of feruloyl esterases in mesoporous materials leads to improved transesterification yield (2011), J. Mol. Catal. B, 72, 57-64.
No PubMed abstract available

Application

EC Number Application Comment Organism
3.1.1.73 synthesis feruloyl esterase, immobilized on mesoporous silica, is useful in the development of biocatalysts for customization of the antioxidant properties of hydroxycinnamic acids Humicola sp.

Protein Variants

EC Number Protein Variants Comment Organism
3.1.1.73 additional information immobilization of feruloyl esterase on mesoporous silica as support. Immobilization in mesoporous silica with larger pore size of 9 nm shows higher protein loading and higher specific activity compared to immobilization with smaller pore size of 5 nm. Adsorption into mesoporous silica changes the product specificity of the enzymes to favor transesterification and decrease the rate of hydrolysis compared to free enzymes, overview. The immobilized enzyme has a butyl ferulate yield of up to 90%, significantly higher compared to free enzyme. Additionally, the immobilized enzymes shows an excellent operational stability and reusability, retaining 70% or more of the initial activity after 6 sequential runs, each lasting 6 days Humicola sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.73 methyl 4-coumarate + H2O Humicola sp.
-
 4-coumaric acid + methanol
-
 ?
3.1.1.73 methyl caffeate + H2O Humicola sp.
-
 caffeic acid + methanol
-
 ?
3.1.1.73 methyl ferulate + H2O Humicola sp.
-
 ferulic acid + methanol
-
 ?
3.1.1.73 methyl sinapate + H2O Humicola sp.
-
 sinapic acid + methanol
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.73 Humicola sp.
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.73 commercial preparation crude enzyme preparation Depol 740L Humicola sp.
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.73 additional information
-
 specific transesterification activity of free and immobilized enzymes, overview Humicola sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.73 methyl 4-coumarate + H2O
-
 Humicola sp. 4-coumaric acid + methanol
-
 ?
3.1.1.73 methyl caffeate + H2O
-
 Humicola sp. caffeic acid + methanol
-
 ?
3.1.1.73 methyl ferulate + H2O
-
 Humicola sp. ferulic acid + methanol
-
 ?
3.1.1.73 methyl sinapate + H2O
-
 Humicola sp. sinapic acid + methanol
-
 ?
3.1.1.73 additional information the enzyme performs transesterification of methyl ferulate with 1-butanol into butyl ferulate and methanol Humicola sp. ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.1.73 FAE
-
 Humicola sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.73 50
-
 assay at Humicola sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.73 6
-
 assay at Humicola sp.