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Literature summary extracted from

  • Chen, S.; Su, L.; Billig, S.; Zimmermann, W.; Chen, J.; Wu, J.
    Biochemical characterization of the cutinases from Thermobifida fusca (2010), J. Mol. Catal. B, 63, 121-127.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.74 expression of cutinase FspC in Bacillus subtilis Fusarium vanettenii
3.1.1.74 expression of cutinase isozymes Tfu 0882 and Tfu 0883 in Escherichia coli strain BL21(DE3) Thermobifida fusca

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.74 Ba2+ inhibits isozyme Tfu 0882 7% Thermobifida fusca
3.1.1.74 Ca2+ inhibits 6% at 1 mM Fusarium vanettenii
3.1.1.74 Ca2+ inhibits isozyme Tfu 0882 28% and isozyme Tfu 0883 18% at 1 mM Thermobifida fusca
3.1.1.74 Cr3+ inhibits 72% at 1 mM Fusarium vanettenii
3.1.1.74 Cr3+ inhibits isozyme Tfu 0882 89% and isozyme Tfu 0883 90% at 1 mM Thermobifida fusca
3.1.1.74 Cu2+ inhibits 17% at 1 mM Fusarium vanettenii
3.1.1.74 Fe2+ inhibits 54% at 1 mM Fusarium vanettenii
3.1.1.74 Fe2+ inhibits isozyme Tfu 0882 45% and isozyme Tfu 0883 36% at 1 mM Thermobifida fusca
3.1.1.74 Hg2+ inhibits completely at 1 mM Fusarium vanettenii
3.1.1.74 Hg2+ inhibits isozyme Tfu 0882 and isozyme Tfu 0883 completely at 1 mM Thermobifida fusca
3.1.1.74 Mg2+ inhibits 11% at 1 mM Fusarium vanettenii
3.1.1.74 Mg2+ inhibits isozyme Tfu 0882 34% Thermobifida fusca
3.1.1.74 Ni2+ inhibits 7% at 1 mM Thermobifida fusca
3.1.1.74 Pb2+ inhibits 39% at 1 mM Fusarium vanettenii
3.1.1.74 Pb2+ inhibits isozyme Tfu 0882 48% and isozyme Tfu 0883 52% at 1 mM Thermobifida fusca
3.1.1.74 Zn2+ inhibits 18% at 1 mM Fusarium vanettenii
3.1.1.74 Zn2+ inhibits isozyme Tfu 0882 50% and isozyme Tfu 0883 56% at 1 mM Thermobifida fusca

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.74 0.272
-
 4-nitrophenyl butyrate pH 8.0, 60°C, recombinant cutinase FspC Fusarium vanettenii
3.1.1.74 0.505
-
 4-nitrophenyl butyrate pH 8.0, 60°C, isozyme Tfu 0883 Thermobifida fusca
3.1.1.74 0.673
-
 4-nitrophenyl butyrate pH 8.0, 60°C, isozyme Tfu 0882 Thermobifida fusca

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.1.74 Ba2+ activates isozyme Tfu 0883 7% at 1 mM Thermobifida fusca
3.1.1.74 Co2+ activates 7% at 1 mM Fusarium vanettenii
3.1.1.74 Co2+ activates isozyme Tfu 0882 4% and isozyme Tfu 0883 24% at 1 mM Thermobifida fusca
3.1.1.74 Mg2+ activates isozyme Tfu 0883 11% at 1 mM Thermobifida fusca
3.1.1.74 Mn2+ activates 36% at 1 mM Fusarium vanettenii
3.1.1.74 Mn2+ activates isozyme Tfu 0882 9% and isozyme Tfu 0883 24% at 1 mM Thermobifida fusca
3.1.1.74 additional information Ba2+ and EDTA have no effect on enzyme activity at 1 mM and 10 mM, respectively Thermobifida fusca
3.1.1.74 additional information EDTA has no effect on enzyme activity at 10 mM Fusarium vanettenii
3.1.1.74 Ni2+ activates isozyme Tfu 0882 5% and isozyme Tfu 0883 7% at 1 mM Fusarium vanettenii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.74 28997
-
 1 * 29000, isozymes Tfu 0882 and Tfu 0883, SDS-PAGE, 1 * 29220, isozyme Tfu 0882, sequence calculation, 1 * 28997, isozyme Tfu 0883, sequence calculation Thermobifida fusca
3.1.1.74 29000
-
 1 * 29000, isozymes Tfu 0882 and Tfu 0883, SDS-PAGE, 1 * 29220, isozyme Tfu 0882, sequence calculation, 1 * 28997, isozyme Tfu 0883, sequence calculation Thermobifida fusca
3.1.1.74 29220
-
 1 * 29000, isozymes Tfu 0882 and Tfu 0883, SDS-PAGE, 1 * 29220, isozyme Tfu 0882, sequence calculation, 1 * 28997, isozyme Tfu 0883, sequence calculation Thermobifida fusca
3.1.1.74 32000
-
 recombinant isozymes Tfu 0882 and Tfu 0883, gel filtration Thermobifida fusca

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.74 additional information Thermobifida fusca the enzyme exhibits a broad substrate specificity against plant cutin, synthetic polyesters, insoluble triglycerides, and soluble esters ?
-
 ?
3.1.1.74 additional information Fusarium vanettenii the enzyme exhibits a broad substrate specificity against plant cutin, synthetic polyesters, insoluble triglycerides, and soluble esters ?
-
 ?

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
3.1.1.74 additional information stability of the cutinase in different organic solvents. The cutinase is incubated with 75% v/v of organic solvent in assay buffer at 20°C for 18 h, overview Fusarium vanettenii
3.1.1.74 additional information stability of the cutinase in different organic solvents. The cutinase isozymes are incubated with 75% v/v of organic solvent in assay buffer at 20°C for 18 h, overview Thermobifida fusca

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.74 Fusarium vanettenii
-
-
-
3.1.1.74 Thermobifida fusca
-
 two isozymes Tfu 0882 and Tfu 0883
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.74 recombinant cutinase FspC from Bacillus subtilis Fusarium vanettenii
3.1.1.74 recombinant cutinase isozymes Tfu 0882 and Tfu 0883 from Escherichia coli strain BL21(DE3) Thermobifida fusca

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.74 4-nitrophenyl butyrate + H2O
-
 Thermobifida fusca 4-nitrophenol + butyrate
-
 ?
3.1.1.74 4-nitrophenyl butyrate + H2O
-
 Fusarium vanettenii 4-nitrophenol + butyrate
-
 ?
3.1.1.74 4-nitrophenyl palmitate + H2O
-
 Thermobifida fusca 4-nitrophenol + palmitate
-
 ?
3.1.1.74 4-nitrophenyl palmitate + H2O
-
 Fusarium vanettenii 4-nitrophenol + palmitate
-
 ?
3.1.1.74 additional information the enzyme exhibits a broad substrate specificity against plant cutin, synthetic polyesters, insoluble triglycerides, and soluble esters Thermobifida fusca ?
-
 ?
3.1.1.74 additional information the enzyme exhibits a broad substrate specificity against plant cutin, synthetic polyesters, insoluble triglycerides, and soluble esters Fusarium vanettenii ?
-
 ?
3.1.1.74 tributyrin + H2O
-
 Thermobifida fusca ?
-
 ?
3.1.1.74 tributyrin + H2O
-
 Fusarium vanettenii ?
-
 ?
3.1.1.74 triolein + H2O
-
 Thermobifida fusca ?
-
 ?
3.1.1.74 triolein + H2O
-
 Fusarium vanettenii ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.74 monomer
-
 Fusarium vanettenii
3.1.1.74 monomer 1 * 29000, isozymes Tfu 0882 and Tfu 0883, SDS-PAGE, 1 * 29220, isozyme Tfu 0882, sequence calculation, 1 * 28997, isozyme Tfu 0883, sequence calculation Thermobifida fusca

Synonyms

EC Number Synonyms Comment Organism
3.1.1.74 FspC
-
 Fusarium vanettenii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.74 40
-
-
 Fusarium vanettenii
3.1.1.74 60
-
-
 Thermobifida fusca

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.1.74 483
-
 4-nitrophenyl butyrate pH 8.0, 60°C, isozyme Tfu 0882 Thermobifida fusca
3.1.1.74 742
-
 4-nitrophenyl butyrate pH 8.0, 60°C, isozyme Tfu 0883 Thermobifida fusca
3.1.1.74 837
-
 4-nitrophenyl butyrate pH 8.0, 60°C, recombinant cutinase FspC Fusarium vanettenii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.74 8
-
 assay at Thermobifida fusca
3.1.1.74 8
-
 assay at Fusarium vanettenii

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.1.1.74 150
-
 4-nitrophenyl butyrate pH 8.0, 60°C, isozyme Tfu 0883 Thermobifida fusca
3.1.1.74 700
-
 4-nitrophenyl butyrate pH 8.0, 60°C, isozyme Tfu 0882 Thermobifida fusca
3.1.1.74 3200
-
 4-nitrophenyl butyrate pH 8.0, 60°C, recombinant cutinase FspC Fusarium vanettenii