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Literature summary extracted from

  • French, J.B.; Neau, D.B.; Ealick, S.E.
    Characterization of the structure and function of Klebsiella pneumoniae allantoin racemase (2011), J. Mol. Biol., 410, 447-460.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.1.99.3 expressed in Escherichia coli B834(DE3) cells Klebsiella pneumoniae subsp. pneumoniae

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
5.1.99.3 hanging drop vapor diffusion method, using 24-34% (w/v) polyethylene glycol 400 with 0.1 M sodium acetate, pH 3.7-4.1 Klebsiella pneumoniae subsp. pneumoniae

Protein Variants

EC Number Protein Variants Comment Organism
5.1.99.3 C184S the mutation leads to total loss of activity Klebsiella pneumoniae subsp. pneumoniae
5.1.99.3 C79S the mutation leads to total loss of activity Klebsiella pneumoniae subsp. pneumoniae
5.1.99.3 C79S/C184S the double mutation leads to total loss of activity Klebsiella pneumoniae subsp. pneumoniae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.1.99.3 0.3
-
 (S)-allantoin in 10 mM NaCl and 10 mM HEPES, pH 7.5, at 25°C Klebsiella pneumoniae subsp. pneumoniae
5.1.99.3 0.4
-
 (R)-allantoin in 10 mM NaCl and 10 mM HEPES, pH 7.5, at 25°C Klebsiella pneumoniae subsp. pneumoniae

Organism

EC Number Organism UniProt Comment Textmining
5.1.99.3 Klebsiella pneumoniae subsp. pneumoniae
-
-
-
5.1.99.3 Klebsiella pneumoniae subsp. pneumoniae MGH78578
-
-
-
5.1.99.3 Klebsiella pneumoniae subsp. pneumoniae Trevisan MGH78578
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.1.99.3 Ni-NTA resin column chromatography, gel filtration Klebsiella pneumoniae subsp. pneumoniae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.1.99.3 (R)-allantoin
-
 Klebsiella pneumoniae subsp. pneumoniae (S)-allantoin
-
 r
5.1.99.3 (R)-allantoin
-
 Klebsiella pneumoniae subsp. pneumoniae Trevisan MGH78578 (S)-allantoin
-
 r
5.1.99.3 (R)-allantoin
-
 Klebsiella pneumoniae subsp. pneumoniae MGH78578 (S)-allantoin
-
 r
5.1.99.3 (S)-allantoin
-
 Klebsiella pneumoniae subsp. pneumoniae (R)-allantoin
-
 r
5.1.99.3 (S)-allantoin
-
 Klebsiella pneumoniae subsp. pneumoniae Trevisan MGH78578 (R)-allantoin
-
 r
5.1.99.3 (S)-allantoin
-
 Klebsiella pneumoniae subsp. pneumoniae MGH78578 (R)-allantoin
-
 r

Subunits

EC Number Subunits Comment Organism
5.1.99.3 hexamer trimer of dimers, X-ray crystallography Klebsiella pneumoniae subsp. pneumoniae

Synonyms

EC Number Synonyms Comment Organism
5.1.99.3 HpxA
-
 Klebsiella pneumoniae subsp. pneumoniae

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.1.99.3 183
-
 (S)-allantoin in 10 mM NaCl and 10 mM HEPES, pH 7.5, at 25°C Klebsiella pneumoniae subsp. pneumoniae
5.1.99.3 238
-
 (R)-allantoin in 10 mM NaCl and 10 mM HEPES, pH 7.5, at 25°C Klebsiella pneumoniae subsp. pneumoniae

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
5.1.99.3 595
-
 (R)-allantoin in 10 mM NaCl and 10 mM HEPES, pH 7.5, at 25°C Klebsiella pneumoniae subsp. pneumoniae
5.1.99.3 610
-
 (S)-allantoin in 10 mM NaCl and 10 mM HEPES, pH 7.5, at 25°C Klebsiella pneumoniae subsp. pneumoniae