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Literature summary extracted from

  • Ki, M.R.; Yun, S.K.; Choi, K.M.; Hwang, S.Y.
    Identification and characterization of the acid phosphatase HppA in Helicobacter pylori (2011), J. Microbiol. Biotechnol., 21, 483-493.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.2 Ca2+ inhibits enzymatic activity Helicobacter pylori
3.1.3.2 Mn2+ inhibits enzymatic activity Helicobacter pylori
3.1.3.2 Zn2+ inhibits enzymatic activity Helicobacter pylori

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.2 0.12
-
 4-nitrophenyl phosphate pH 6.0, 37°C, periplasmic enzyme with 0.1 mM NiCl2 Helicobacter pylori
3.1.3.2 0.43
-
 4-nitrophenyl phosphate pH 6.0, 37°C, periplasmic enzyme without Ni2+ Helicobacter pylori
3.1.3.2 0.67
-
 4-nitrophenyl phosphate pH 6.0, 37°C, periplasmic enzyme with 0.1 mM NiCl2 Helicobacter pylori
3.1.3.2 0.76
-
 4-nitrophenyl phosphate pH 6.0, 37°C, membrane-bound enzyme Helicobacter pylori
3.1.3.2 0.79
-
 ATP pH 6.0, 37°C, membrane-bound enzyme Helicobacter pylori
3.1.3.2 4.14
-
 4-nitrophenyl phosphate pH 6.0, 37°C, periplasmic enzyme without Ni2+ Helicobacter pylori

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.3.2 membrane
-
 Helicobacter pylori 16020
-
3.1.3.2 periplasm
-
 Helicobacter pylori
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.2 K+ under acidic conditions (pH below 4.5) periplasmic enzymatic activity is dependent on the presence of mono- and/or divalent cations. Hydrolsis of 4-nitrophenyl phosphate is enhanced but not hydrolsis of ATP Helicobacter pylori
3.1.3.2 NH4+ under acidic conditions (pH below 4.5) periplasmic enzymatic activity is dependent on the presence of mono- and/or divalent cations Helicobacter pylori
3.1.3.2 Ni2+ under acidic conditions (pH below 4.5) periplasmic enzymatic activity is dependent on the presence of mono- and/or divalent cations. Ni2+ lowers Km without changing Vmax Helicobacter pylori

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.2 24000
-
 SDS-PAGE, periplasmic fraction Helicobacter pylori
3.1.3.2 27000
-
 SDS-PAGE, membrane-bound form Helicobacter pylori
3.1.3.2 72000
-
 trimeric native from of the enzyme Helicobacter pylori

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.2 Helicobacter pylori
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.2 purified 192- and 34fold from the periplasmic and membrane fractions of Helicobacter pylori. Purification steps of membrane enzyme: Solubilization, DEAE-Sepharose and Phe-Sepharose. Purification steps of periplasmic enzyme: (NH4)2SO4 precipitation, SP-Sepharose, Phe-Sepharose and Q-Sepharose Helicobacter pylori

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.2 4-nitrophenyl phosphate + H2O
-
 Helicobacter pylori 4-nitrophenol + phosphate
-
 ?
3.1.3.2 ATP + H2O periplasmic enzyme: stronger hydrolysis at pH 5.5 than at pH 6.0 Helicobacter pylori ADP + phosphate
-
 ?
3.1.3.2 CTP + H2O periplasmic enzyme: stronger hydrolysis at pH 6.0 than at pH 5.5 Helicobacter pylori CDP + phosphate
-
 ?
3.1.3.2 TTP + H2O periplasmic enzyme: stronger hydrolysis at pH 6.0 than at pH 5.5 Helicobacter pylori TDP + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.2 trimer
-
 Helicobacter pylori

Synonyms

EC Number Synonyms Comment Organism
3.1.3.2 HppA
-
 Helicobacter pylori

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.3.2 37
-
 assay at Helicobacter pylori

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.2 5.5
-
 periplasmic enzyme: adenosine nucleotides are better hydrolyzed at pH 5.5 than at pH 6.0 Helicobacter pylori
3.1.3.2 6
-
 assay at Helicobacter pylori
3.1.3.2 6
-
 periplasmic enzyme: CTP and TTP are better hydrolyzed at pH 6 than at pH 5.5 Helicobacter pylori

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.3.2 4.5
-
 periplasmic enzyme is inactive at pH below 4.5 but displays activity in the presence of NH4Cl and NiCl2 Helicobacter pylori

General Information

EC Number General Information Comment Organism
3.1.3.2 malfunction HppA knockout cells grow more slowly. Knockout cells import Mg2+ at a markedly lowered rate, but assimilate urea rapidly, with a subsequent increase in extracellular pH. Moreover, mutant cells are much more sensitive to extracellular potassium ions, as well as to metronidazole, omeprazole, or thiophenol, with considerably lowered MIC values, than wild-type cells Helicobacter pylori