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Literature summary extracted from

  • Igarashi, M.; Osuga, J.; Isshiki, M.; Sekiya, M.; Okazaki, H.; Takase, S.; Takanashi, M.; Ohta, K.; Kumagai, M.; Nishi, M.; Fujita, T.; Nagai, R.; Kadowaki, T.; Ishibashi, S.
    Targeting of neutral cholesterol ester hydrolase to the endoplasmic reticulum via its N-terminal sequence (2010), J. Lipid Res., 51, 274-285.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.13 GFP-tagged enzyme is overexpressed in RAW264.7 cells Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
3.1.1.13 N270Q enzyme activity level of the mutant is significantly reduced compared with wild type Homo sapiens
3.1.1.13 N367Q enzyme activity level of the mutant is significantly reduced compared with wild type Homo sapiens
3.1.1.13 N389Q the mutant shows incomplete glycosylation Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.1.13 endoplasmic reticulum
-
 Homo sapiens 5783
-
3.1.1.13 membrane NCEH is a single-membrane-spanning type II membrane protein Homo sapiens 16020
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.13 40000
-
 x * 40000, SDS-PAGE Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.13 Homo sapiens
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.1.1.13 glycoprotein
-
 Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.13 HEK-293 cell
-
 Homo sapiens
-
3.1.1.13 HeLa cell
-
 Homo sapiens
-
3.1.1.13 macrophage
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.13 cholesteryl oleate + H2O
-
 Homo sapiens cholesterol + oleate
-
 ?
3.1.1.13 additional information neutral cholesterol ester hydrolase is not a target of protein kinase A Homo sapiens ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.13 ? x * 40000, SDS-PAGE Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
3.1.1.13 NCEH
-
 Homo sapiens
3.1.1.13 neutral cholesterol ester hydrolase
-
 Homo sapiens

General Information

EC Number General Information Comment Organism
3.1.1.13 physiological function NCEH hydrolyzes cholesterol ester to deliver cholesterol for efflux out of the macrophage cells Homo sapiens