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Literature summary extracted from

  • Ahuja, I.; Borgen, B.H.; Hansen, M.; Honne, B.I.; Mueller, C.; Rohloff, J.; Rossiter, J.T.; Bones, A.M.
    Oilseed rape seeds with ablated defence cells of the glucosinolate-myrosinase system. Production and characteristics of double haploid MINELESS plants of Brassica napus L. (2011), J. Exp. Bot., 62, 4975-4993.
    View publication on PubMedView publication on EuropePMC

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.147 65000
-
 x * 65000, SDS-PAGE. Immunoblot analysis reveals the major bands of myrosinase polypeptide classes (denoted as 65, 70, and 75 kDa) in wild type samples of seeds Brassica napus
3.2.1.147 70000
-
 x * 70000, SDS-PAGE. Immunoblot analysis reveals the major bands of myrosinase polypeptide classes (denoted as 65, 70, and 75 kDa) in wild type samples of seeds Brassica napus
3.2.1.147 75000
-
 x * 75000, SDS-PAGE. Immunoblot analysis reveals the major bands of myrosinase polypeptide classes (denoted as 65, 70, and 75 kDa) in wild type samples of seeds Brassica napus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.147 Brassica napus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.147 myrosin cell
-
 Brassica napus
-
3.2.1.147 seed
-
 Brassica napus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.147 additional information
-
 the specific myrosinase activity in wild type seeds ranges from 0.1186-0.4263 micromol glucose/min/mg protein Brassica napus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.147 sinigrin + H2O
-
 Brassica napus D-glucose + isothiocyanate + ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.147 ? x * 65000, SDS-PAGE. Immunoblot analysis reveals the major bands of myrosinase polypeptide classes (denoted as 65, 70, and 75 kDa) in wild type samples of seeds Brassica napus
3.2.1.147 ? x * 70000, SDS-PAGE. Immunoblot analysis reveals the major bands of myrosinase polypeptide classes (denoted as 65, 70, and 75 kDa) in wild type samples of seeds Brassica napus
3.2.1.147 ? x * 75000, SDS-PAGE. Immunoblot analysis reveals the major bands of myrosinase polypeptide classes (denoted as 65, 70, and 75 kDa) in wild type samples of seeds Brassica napus

Synonyms

EC Number Synonyms Comment Organism
3.2.1.147 myrosinase
-
 Brassica napus

General Information

EC Number General Information Comment Organism
3.2.1.147 malfunction double haploid myrosin cell-free plants (MINELESS plants) have significantly reduced myrosinase levels and glucosinolate hydrolysis products Brassica napus
3.2.1.147 physiological function Brassica napus contains a defence system known as the glucosinolate-myrosinase system or the mustard oil bomb. The mustard oil bomb which includes myrosinase and glucosinolates is triggered by abiotic and biotic stress, resulting in the formation of toxic products such as nitriles and isothiocyanates Brassica napus