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Literature summary extracted from

  • Marcus, Y.; Altman-Gueta, H.; Wolff, Y.; Gurevitz, M.
    Rubisco mutagenesis provides new insight into limitations on photosynthesis and growth in Synechocystis PCC6803 (2011), J. Exp. Bot., 62, 4173-4182.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.1.1.39 phosphate stimulates the activation of Rubisco, all three phosphate-binding sites are involved in stimulation of Rubisco activation Synechocystis sp.

Protein Variants

EC Number Protein Variants Comment Organism
4.1.1.39 G404A while phosphate-stimulated Rubisco activation diminishes for the mutant, inhibition of catalysis by phosphate remains unchanged. The photosynthesis and growth rate under saturating irradiance and inorganic carbon concentration is not reduced, the mutant shows a marked reductions in kcat compared to the wild type enzyme Synechocystis sp.
4.1.1.39 T65A while phosphate-stimulated Rubisco activation diminishes for the mutant, inhibition of catalysis by phosphate remains unchanged. The photosynthesis and growth rate under saturating irradiance and inorganic carbon concentration is reduced 40-50%, the mutant shows a marked reductions in kcat compared to the wild type enzyme Synechocystis sp.
4.1.1.39 T65S while phosphate-stimulated Rubisco activation diminishes for the mutant, inhibition of catalysis by phosphate remains unchanged. The photosynthesis and growth rate under saturating irradiance and inorganic carbon concentration is reduced 40-50%, the mutant shows a marked reductions in kcat compared to the wild type enzyme Synechocystis sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.1.39 phosphate inhibits the catalysis of Rubisco, only the 5P-site is involved in inhibition of catalysis Synechocystis sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.1.39 0.0463
-
 D-ribulose 1,5-bisphosphate mutant enzyme G404A, in 50 mM HEPES, pH 8.0, at 30°C Synechocystis sp.
4.1.1.39 0.0473
-
 D-ribulose 1,5-bisphosphate mutant enzyme T65S, in 50 mM HEPES, pH 8.0, at 30°C Synechocystis sp.
4.1.1.39 0.146
-
 D-ribulose 1,5-bisphosphate wild type enzyme, in 50 mM HEPES, pH 8.0, at 30°C Synechocystis sp.
4.1.1.39 0.193
-
 D-ribulose 1,5-bisphosphate mutant enzyme T65A, in 50 mM HEPES, pH 8.0, at 30°C Synechocystis sp.
4.1.1.39 0.258
-
 CO2 mutant enzyme T65S, in 50 mM HEPES, pH 8.0, at 30°C Synechocystis sp.
4.1.1.39 0.268
-
 CO2 wild type enzyme, in 50 mM HEPES, pH 8.0, at 30°C Synechocystis sp.
4.1.1.39 0.283
-
 CO2 mutant enzyme T65A, in 50 mM HEPES, pH 8.0, at 30°C Synechocystis sp.
4.1.1.39 0.296
-
 CO2 mutant enzyme G404A, in 50 mM HEPES, pH 8.0, at 30°C Synechocystis sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.1.39 Mg2+ the enzyme is activated for 30 min by 0.28 mM CO2 and 10 mM MgCl2 at 30°C Synechocystis sp.

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.39 Synechocystis sp.
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.39 partial purification by ammonium sulfate precipitation and Sephadex G-100 gel filtration Synechocystis sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.39 D-ribulose 1,5-bisphosphate + CO2 + H2O
-
 Synechocystis sp. 3-phospho-D-glycerate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.1.1.39 ribulose-1,5-bisphosphate carboxylase/oxygenase
-
 Synechocystis sp.
4.1.1.39 Rubisco
-
 Synechocystis sp.

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.1.1.39 0.67
-
 D-ribulose 1,5-bisphosphate mutant enzyme T65A, in 50 mM HEPES, pH 8.0, at 30°C Synechocystis sp.
4.1.1.39 1.2
-
 D-ribulose 1,5-bisphosphate mutant enzyme G404A, in 50 mM HEPES, pH 8.0, at 30°C Synechocystis sp.
4.1.1.39 2.2
-
 D-ribulose 1,5-bisphosphate mutant enzyme T65S, in 50 mM HEPES, pH 8.0, at 30°C Synechocystis sp.
4.1.1.39 14.28
-
 D-ribulose 1,5-bisphosphate wild type enzyme, in 50 mM HEPES, pH 8.0, at 30°C Synechocystis sp.

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
4.1.1.39 8.3
-
 phosphate wild type enzyme, in 50 mM HEPES, pH 8.0, at 30°C Synechocystis sp.
4.1.1.39 8.35
-
 phosphate mutant enzyme G404A, in 50 mM HEPES, pH 8.0, at 30°C Synechocystis sp.
4.1.1.39 9.02
-
 phosphate mutant enzyme T65A, in 50 mM HEPES, pH 8.0, at 30°C Synechocystis sp.