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Literature summary extracted from

  • Ariza, A.; Ekloef, J.M.; Spadiut, O.; Offen, W.A.; Roberts, S.M.; Besenmatter, W.; Friis, E.P.; Skjot, M.; Wilson, K.S.; Brumer, H.; Davies, G.
    Structure and activity of Paenibacillus polymyxa xyloglucanase from glycoside hydrolase family 44 (2011), J. Biol. Chem., 286, 33890-33900.
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.2.1.151 hanging drop vapor diffusion method, using 0.1 M bis-Tris, pH 6.5, 0.2 M Li2SO4, 25% (w/v) PEG 3350 Paenibacillus polymyxa

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.151 E358S nucleophile mutant Paenibacillus polymyxa
3.2.1.151 K129A/R156Y increased stability mutant Paenibacillus polymyxa

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.151 beta-1,4-cellobiosyloxazine competitive inhibitor Paenibacillus polymyxa
3.2.1.151 beta-1,4-glucosyl-noeuromycin competitive inhibitor Paenibacillus polymyxa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.151 0.029
-
 2-chloro-4-nitrophenyl Glcbeta(1->4)Glcbeta(1->4)Glcbeta(1->4)Glcbeta apparent value, at pH 6.0 and 30°C Paenibacillus polymyxa
3.2.1.151 0.134
-
 Xylalpha(1->6)Glcbeta(1->4)Xylalpha(1->6)Glcbeta(1->4)Xylalpha(1->6)Glcbeta(1->4)Glcbeta(1->4)Xylalpha(1->6)Glcbeta(1->4)Xylalpha(1->6)Glcbeta(1->4)Xylalpha(1->6)Glcbeta(1->4)Glcbeta apparent value, at pH 6.0 and 30°C Paenibacillus polymyxa
3.2.1.151 0.361
-
 2-chloro-4-nitrophenyl Glcbeta(1->4)Glcbeta(1->4)Glcbeta apparent value, at pH 6.0 and 30°C Paenibacillus polymyxa
3.2.1.151 0.998
-
 Xylalpha(1->6)Glcbeta(1->4)[Galbeta(1->2)Xylalpha(1->6)]Glcbeta(1->4)[Galbeta(1->2)Xylalpha(1->6)]Glcbeta(1->4)Glcbeta apparent value, at pH 6.0 and 30°C Paenibacillus polymyxa

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.151 Paenibacillus polymyxa
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.151 2-chloro-4-nitrophenyl Glcbeta(1->4)Glcbeta(1->4)Glcbeta + H2O 3.8% activity compared to 2-chloro-4-nitrophenyl-Glcbeta(1->4)Glcbeta(1->4)Glcbeta(1->4)Glcbeta Paenibacillus polymyxa 2-chloro-4-nitrophenol + Glcbeta(1->4)Glcbeta(1->4)Glcbeta
-
 ?
3.2.1.151 2-chloro-4-nitrophenyl Glcbeta(1->4)Glcbeta(1->4)Glcbeta(1->4)Glcbeta + H2O 100% activity Paenibacillus polymyxa 2-chloro-4-nitrophenol + Glcbeta(1->4)Glcbeta(1->4)Glcbeta(1->4)Glcbeta
-
 ?
3.2.1.151 2-chloro-4-nitrophenyl Glcbeta(1->4)Glcbeta(1->4)Glcbeta(1->4)Glcbeta + H2O
-
 Paenibacillus polymyxa ?
-
 ?
3.2.1.151 2-chloro-4-nitrophenyl Xylalpha(1->6)Glcbeta(1->4)[Galbeta(1->2)Xylalpha(1->6)]Glcbeta(1->4)[Galbeta(1->2)Xylalpha(1->6)]Glcbeta(1->4)Glcbeta + H2O 1.3% activity compared to 2-chloro-4-nitrophenyl-Glcbeta(1->4)Glcbeta(1->4)Glcbeta(1->4)Glcbeta Paenibacillus polymyxa 2-chloro-4-nitrophenol + ?
-
 ?
3.2.1.151 2-chloro-4-nitrophenyl Xylalpha(1->6)Glcbeta(1->4)[Galbeta(1->2)Xylalpha(1->6)]Glcbeta(1->4)[Galbeta(1->2)Xylalpha(1->6)]Glcbeta(1->4)Glcbeta + H2O
-
 Paenibacillus polymyxa ?
-
 ?
3.2.1.151 2-chloro-4-nitrophenyl Xylalpha(1->6)Glcbeta(1->4)[Xylalpha(1->6)]Glcbeta(1->4)[Xylalpha(1->6)]Glcbeta(1->4)Glcbeta + H2O 19.4% activity compared to 2-chloro-4-nitrophenyl-Glcbeta(1->4)Glcbeta(1->4)Glcbeta(1->4)Glcbeta Paenibacillus polymyxa 2-chloro-4-nitrophenol + ?
-
 ?
3.2.1.151 barley beta-glucan + H2O 87% activity compared to tamarind xyloglucan Paenibacillus polymyxa ?
-
 ?
3.2.1.151 carboxymethylcellulose + H2O high activity Paenibacillus polymyxa ?
-
 ?
3.2.1.151 carob galactomannan + H2O 4% activity compared to tamarind xyloglucan Paenibacillus polymyxa ?
-
 ?
3.2.1.151 cellohexaose + H2O
-
 Paenibacillus polymyxa ?
-
 ?
3.2.1.151 cellopentaose + H2O
-
 Paenibacillus polymyxa ?
-
 ?
3.2.1.151 cellotetraose + H2O
-
 Paenibacillus polymyxa ?
-
 ?
3.2.1.151 Glcbeta(1->4)[Xylalpha(1->6)Glcbeta(1->4)[Xylalpha(1->6)]Glcbeta(1->4)Glcbeta(1->4)[Xylalpha(1->6)]Glcbeta(1->4)[Xylalpha(1->6)]Glcbeta(1->4)[Xylalpha(1->6)]Glcbeta(1->4)Glcbeta + H2O
-
 Paenibacillus polymyxa ?
-
 ?
3.2.1.151 hydroxyethyl cellulose + H2O 65% activity compared to tamarind xyloglucan Paenibacillus polymyxa ?
-
 ?
3.2.1.151 additional information no activity with 2-chloro-4-nitrophenyl Glcbeta(1->4)Glcbeta, cellotriose, and cellobiose Paenibacillus polymyxa ?
-
 ?
3.2.1.151 additional information the enzyme demonstrates a broad substrate specificity for polysaccharides containing beta-1,4 linkages and displays an unusual specificity on defined xyloglucan oligosaccharides, cleaving the XXXG-XXXG repeat into XXX and GXXXG Paenibacillus polymyxa ?
-
 ?
3.2.1.151 tamarind xyloglucan + H2O highest activity (100%) Paenibacillus polymyxa ?
-
 ?
3.2.1.151 wheat arabinoxylan + H2O 15% activity compared to tamarind xyloglucan Paenibacillus polymyxa ?
-
 ?
3.2.1.151 Xylalpha(1->6)Glcbeta(1->4)Xylalpha(1->6)Glcbeta(1->4)Xylalpha(1->6)Glcbeta(1->4)Glcbeta(1->4)Xylalpha(1->6)Glcbeta(1->4)Xylalpha(1->6)Glcbeta(1->4)Xylalpha(1->6)Glcbeta(1->4)Glcbeta + H2O
-
 Paenibacillus polymyxa ?
-
 ?
3.2.1.151 Xylalpha(1->6)Glcbeta(1->4)[Galbeta(1->2)Xylalpha(1->6)]Glcbeta(1->4)[Galbeta(1->2)Xylalpha(1->6)]Glcbeta(1->4)Glcbeta + H2O
-
 Paenibacillus polymyxa ?
-
 ?
3.2.1.151 xyloglucan + H2O
-
 Paenibacillus polymyxa xyloglucan oligosaccharides
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.151 endo-beta-1,4-(xylo)glucan hydrolase
-
 Paenibacillus polymyxa
3.2.1.151 XG44
-
 Paenibacillus polymyxa

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.151 6.96
-
 Xylalpha(1->6)Glcbeta(1->4)[Galbeta(1->2)Xylalpha(1->6)]Glcbeta(1->4)[Galbeta(1->2)Xylalpha(1->6)]Glcbeta(1->4)Glcbeta apparent value, at pH 6.0 and 30°C Paenibacillus polymyxa
3.2.1.151 7.34
-
 2-chloro-4-nitrophenyl Glcbeta(1->4)Glcbeta(1->4)Glcbeta apparent value, at pH 6.0 and 30°C Paenibacillus polymyxa
3.2.1.151 14.01
-
 Xylalpha(1->6)Glcbeta(1->4)Xylalpha(1->6)Glcbeta(1->4)Xylalpha(1->6)Glcbeta(1->4)Glcbeta(1->4)Xylalpha(1->6)Glcbeta(1->4)Xylalpha(1->6)Glcbeta(1->4)Xylalpha(1->6)Glcbeta(1->4)Glcbeta apparent value, at pH 6.0 and 30°C Paenibacillus polymyxa
3.2.1.151 15.93
-
 2-chloro-4-nitrophenyl Glcbeta(1->4)Glcbeta(1->4)Glcbeta(1->4)Glcbeta apparent value, at pH 6.0 and 30°C Paenibacillus polymyxa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.151 6
-
 for hydrolysis of tamarind xyloglucan Paenibacillus polymyxa

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.2.1.151 6.98
-
 Xylalpha(1->6)Glcbeta(1->4)[Galbeta(1->2)Xylalpha(1->6)]Glcbeta(1->4)[Galbeta(1->2)Xylalpha(1->6)]Glcbeta(1->4)Glcbeta apparent value, at pH 6.0 and 30°C Paenibacillus polymyxa
3.2.1.151 20.33
-
 2-chloro-4-nitrophenyl Glcbeta(1->4)Glcbeta(1->4)Glcbeta apparent value, at pH 6.0 and 30°C Paenibacillus polymyxa
3.2.1.151 104.7
-
 Xylalpha(1->6)Glcbeta(1->4)Xylalpha(1->6)Glcbeta(1->4)Xylalpha(1->6)Glcbeta(1->4)Glcbeta(1->4)Xylalpha(1->6)Glcbeta(1->4)Xylalpha(1->6)Glcbeta(1->4)Xylalpha(1->6)Glcbeta(1->4)Glcbeta apparent value, at pH 6.0 and 30°C Paenibacillus polymyxa
3.2.1.151 541.7
-
 2-chloro-4-nitrophenyl Glcbeta(1->4)Glcbeta(1->4)Glcbeta(1->4)Glcbeta apparent value, at pH 6.0 and 30°C Paenibacillus polymyxa