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Literature summary extracted from

  • Nakano, C.; Horinouchi, S.; Ohnishi, Y.
    Characterization of a novel sesquiterpene cyclase involved in (+)-caryolan-1-ol biosynthesis in Streptomyces griseus (2011), J. Biol. Chem., 286, 27980-27987.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.1.138 expressed in Streptomyces lividans strain TK21 and as N-terminally histidine-tagged enzyme in Escherichia coli BL21(DE3) cells Streptomyces griseus
4.2.3.89 expressed in Streptomyces lividans strain TK21 and as N-terminally histidine-tagged enzyme in Escherichia coli BL21(DE3) cells Streptomyces griseus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.3.89 0.0000956
-
 (2E,6E)-farnesyl diphosphate at pH 7.5 and 30°C in the presence of 5 mM MgCl2 Streptomyces griseus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.1.138 Mg2+ more than 5 mM MgCl2 is required for maximum activity. The enzyme reaction is unable to proceed without MgCl2 Streptomyces griseus
4.2.1.138 Mn2+ 38% activity (relative to the activity in the presence ofMg2+) is retained in the presence of 5 mM Mn2+ Streptomyces griseus
4.2.1.138 additional information no activity is observed for Fe2+, Co2+, Zn2+, Ni2+, or Cu2+ Streptomyces griseus
4.2.3.89 Mg2+ more than 5 mM MgCl2 is required for maximum activity. The enzyme reaction is unable to proceed without MgCl2 Streptomyces griseus
4.2.3.89 Mn2+ 38% activity (relative to the activity in the presence of Mg2+) is retained in the presence of 5 mM Mn2+ Streptomyces griseus
4.2.3.89 additional information no activity is observed for Fe2+, Co2+, Zn2+, Ni2+, or Cu2+ Streptomyces griseus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.1.138 41000
-
 x * 41000, SDS-PAGE Streptomyces griseus
4.2.3.89 41000
-
 x * 41000, SDS-PAGE Streptomyces griseus

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.138 Streptomyces griseus
-
-
-
4.2.1.138 Streptomyces griseus IFO 13350
-
-
-
4.2.3.89 Streptomyces griseus
-
-
-
4.2.3.89 Streptomyces griseus IFO 13350
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.138 Ni-NTA column chromatography Streptomyces griseus
4.2.3.89 Ni-NTA column chromatography Streptomyces griseus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.2.1.138 mycelium
-
 Streptomyces griseus
-
4.2.3.89 mycelium
-
 Streptomyces griseus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.138 (+)-beta-caryophyllene + H2O
-
 Streptomyces griseus (+)-caryolan-1-ol
-
 ?
4.2.1.138 (+)-beta-caryophyllene + H2O
-
 Streptomyces griseus IFO 13350 (+)-caryolan-1-ol
-
 ?
4.2.3.89 (2E,6E)-farnesyl diphosphate + H2O
-
 Streptomyces griseus (+)-beta-caryophyllene + diphosphate
-
 ?
4.2.3.89 (2E,6E)-farnesyl diphosphate + H2O
-
 Streptomyces griseus IFO 13350 (+)-beta-caryophyllene + diphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.1.138 ? x * 41000, SDS-PAGE Streptomyces griseus
4.2.3.89 ? x * 41000, SDS-PAGE Streptomyces griseus

Synonyms

EC Number Synonyms Comment Organism
4.2.1.138 GcoA
-
 Streptomyces griseus
4.2.3.89 GcoA
-
 Streptomyces griseus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.3.89 30
-
 at pH 7.5 Streptomyces griseus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.2.3.89 0.025
-
 (2E,6E)-farnesyl diphosphate at pH 7.5 and 30°C in the presence of 5 mM MgCl2 Streptomyces griseus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.3.89 7.5
-
 at 30°C Streptomyces griseus