Literature summary extracted from
Taylor, M.R.; Conrad, J.A.; Wahl, D.; OBrien, P.J.
Kinetic mechanism of human DNA ligase I reveals magnesium-dependent changes in the rate-limiting step that compromise ligation efficiency (2011), J. Biol. Chem., 286, 23054-23062.
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
6.5.1.1 |
the catalytic domain of human LIG1 (residues 232-919) is expressed in Escherichia coli |
Homo sapiens |
Inhibitors
EC Number |
Inhibitors |
Comment |
Organism |
Structure |
---|
6.5.1.1 |
EDTA |
EDTA rapidly inactivates all LIG1-catalyzed reactions |
Homo sapiens |
|
KM Value [mM]
EC Number |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Comment |
Organism |
Structure |
---|
6.5.1.1 |
0.012 |
- |
ATP |
at 30 mM Mg2+, pH and temperature not specified in the publication |
Homo sapiens |
|
Metals/Ions
EC Number |
Metals/Ions |
Comment |
Organism |
Structure |
---|
6.5.1.1 |
Mg2+ |
LIG1 requires multiple Mg2+ ions for catalysis |
Homo sapiens |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
6.5.1.1 |
Homo sapiens |
- |
- |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
6.5.1.1 |
Ni-NTA column chromatography and Q-Sepharose column chromatography |
Homo sapiens |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
6.5.1.1 |
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m |
synthetic 28-mer oligonucleotide duplex that contains a nick with a 3'-hydroxyl and a 5'-phosphate, more than 95% of the nicked DNA is ligated within 2 s |
Homo sapiens |
AMP + diphosphate + (deoxyribonucleotide)m+n |
- |
? |
|
6.5.1.1 |
additional information |
LIG1 catalyzes the ligation of single-strand breaks to complete DNA replication and repair. The energy of ATP is used to form a new phosphodiester bond in DNA via a reaction mechanism that involves three distinct chemical steps: enzyme adenylylation, adenylyl transfer to DNA, and nick sealing |
Homo sapiens |
? |
- |
? |
|
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
6.5.1.1 |
DNA ligase I |
- |
Homo sapiens |
6.5.1.1 |
LIG1 |
- |
Homo sapiens |
Turnover Number [1/s]
EC Number |
Turnover Number Minimum [1/s] |
Turnover Number Maximum [1/s] |
Substrate |
Comment |
Organism |
Structure |
---|
6.5.1.1 |
0.74 |
- |
ATP |
at 30 mM Mg2+, pH and temperature not specified in the publication |
Homo sapiens |
|
Cofactor
EC Number |
Cofactor |
Comment |
Organism |
Structure |
---|
6.5.1.1 |
ATP |
the efficiency of ligation falls from near 100% under optimal conditions with 1 mM Mg2+ and 10 mM ATP to 60% with 1 mM Mg2+ and 2 mM ATP |
Homo sapiens |
|