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Literature summary extracted from

  • Adler, L.N.; Gomez, T.A.; Clarke, S.G.; Linster, C.L.
    A novel GDP-D-glucose phosphorylase involved in quality control of the nucleoside diphosphate sugar pool in Caenorhabditis elegans and mammals (2011), J. Biol. Chem., 286, 21511-21523.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.78 expression in Escherichia coli Caenorhabditis elegans
2.7.7.78 expression in Escherichia coli Homo sapiens
2.7.7.78 expression in Escherichia coli Mus musculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.78 0.002
-
 GDP-alpha-D-glucose pH 7.5, 31°C Homo sapiens
2.7.7.78 0.01
-
 GDP-alpha-D-glucose pH 7.5, 26°C Caenorhabditis elegans
2.7.7.78 1.2
-
 phosphate pH 7.5, 26°C Caenorhabditis elegans
2.7.7.78 2.9
-
 phosphate pH 7.5, 31°C Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.78 GDP-alpha-D-glucose + phosphate Homo sapiens
-
 alpha-D-glucose 1-phosphate + GDP
-
 ?
2.7.7.78 GDP-alpha-D-glucose + phosphate Mus musculus
-
 alpha-D-glucose 1-phosphate + GDP
-
 ?
2.7.7.78 GDP-alpha-D-glucose + phosphate Caenorhabditis elegans the enzyme may be involved in prevention of misincorporation of glucose in place of mannose residues into glycoconjugates i.e. to remove accidentally produced GDP-alpha-D-glucose alpha-D-glucose 1-phosphate + GDP
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.78 Caenorhabditis elegans Q5ZR76
-
-
2.7.7.78 Homo sapiens Q6ZNW5
-
-
2.7.7.78 Mus musculus Q3TLS3
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.7.78 anterior hypodermis
-
 Caenorhabditis elegans
-
2.7.7.78 brain highest levels measured in brain and testis (4-8 times higher than in the remaining tissues) Mus musculus
-
2.7.7.78 embryo developing Caenorhabditis elegans
-
2.7.7.78 heart highest levels measured in brain and testis (4-8 times higher than in the remaining tissues) Mus musculus
-
2.7.7.78 kidney highest levels measured in brain and testis (4-8 times higher than in the remaining tissues) Mus musculus
-
2.7.7.78 larva expressed in all the normal larval stages observed Caenorhabditis elegans
-
2.7.7.78 liver highest levels measured in brain and testis (4-8 times higher than in the remaining tissues) Mus musculus
-
2.7.7.78 spermatheca
-
 Caenorhabditis elegans
-
2.7.7.78 testis highest levels measured in brain and testis (4-8 times higher than in the remaining tissues) Mus musculus
-
2.7.7.78 thymus highest levels measured in brain and testis (4-8 times higher than in the remaining tissues) Mus musculus
-

Storage Stability

EC Number Storage Stability Organism
2.7.7.78 -80°C, 10% glycerol Caenorhabditis elegans
2.7.7.78 -80°C, 10% glycerol Homo sapiens
2.7.7.78 -80°C, 10% glycerol Mus musculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.78 GDP-alpha-D-glucose + phosphate
-
 Homo sapiens alpha-D-glucose 1-phosphate + GDP
-
 ?
2.7.7.78 GDP-alpha-D-glucose + phosphate
-
 Mus musculus alpha-D-glucose 1-phosphate + GDP
-
 ?
2.7.7.78 GDP-alpha-D-glucose + phosphate the enzyme may be involved in prevention of misincorporation of glucose in place of mannose residues into glycoconjugates i.e. to remove accidentally produced GDP-alpha-D-glucose Caenorhabditis elegans alpha-D-glucose 1-phosphate + GDP
-
 ?
2.7.7.78 GDP-alpha-D-glucose + phosphate activities with GDP-L-galactose, GDP-D-mannose and UDP-D-glucose are all less than 3% that with GDP-D-glucose Caenorhabditis elegans alpha-D-glucose 1-phosphate + GDP
-
 ?
2.7.7.78 GDP-alpha-D-glucose + phosphate activities with GDP-L-galactose, GDP-D-mannose and UDP-D-glucose are all less than 3% that with GDP-D-glucose Homo sapiens alpha-D-glucose 1-phosphate + GDP
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.7.78 C10F3.4
-
 Caenorhabditis elegans
2.7.7.78 C15orf58
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.7.78 26
-
 assay at Caenorhabditis elegans

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.7.78 26
-
 GDP-alpha-D-glucose pH 7.5, 31°C Homo sapiens
2.7.7.78 28.1
-
 phosphate pH 7.5, 31°C Homo sapiens
2.7.7.78 265
-
 GDP-alpha-D-glucose pH 7.5, 26°C Caenorhabditis elegans
2.7.7.78 380
-
 phosphate pH 7.5, 26°C Caenorhabditis elegans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.78 7.5
-
 assay at Caenorhabditis elegans

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.7.7.78 9.7
-
 phosphate pH 7.5, 31°C Homo sapiens
2.7.7.78 317
-
 phosphate pH 7.5, 26°C Caenorhabditis elegans
2.7.7.78 1300
-
 GDP-alpha-D-glucose pH 7.5, 31°C Homo sapiens
2.7.7.78 26500
-
 GDP-alpha-D-glucose pH 7.5, 26°C Caenorhabditis elegans