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Literature summary extracted from

  • Keeling, C.I.; Madilao, L.L.; Zerbe, P.; Dullat, H.K.; Bohlmann, J.
    The primary diterpene synthase products of Picea abies levopimaradiene/abietadiene synthase (PaLAS) are epimers of a thermally unstable diterpenol (2011), J. Biol. Chem., 286, 21145-21153.
    View publication on PubMedView publication on EuropePMC

Organism

EC Number Organism UniProt Comment Textmining
4.2.3.18 Picea abies
-
 bifunctional levopimaradiene/abietadiene synthase
-
4.2.3.32 Picea abies Q675L4
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
4.2.3.18 (+)-copalyl diphosphate = abieta-7,13-diene + diphosphate reaction mechanism proceeds via water quenching of the abieta-8(14)-en-13-yl carbocation, resulting in 13-hydroxy-8(14)-abietene Picea abies
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.3.18 (+)-copalyl diphosphate
-
 Picea abies (+-)-13-hydroxy-8(14)-abietene + diphosphate initial product of the reaction, thermally unstable. Dehydration of the alcohol products of (+)-copalyl diphosphate, yielding the well established diterpene products levopimaradiene, abietadiene, neoabietadiene, and palustradiene, may occurr due to three conditions of the GC-MS analysis typically used for identification of diterpene synthase products: a hot injector, a hot oven temperature necessary for eluting the compounds, and the high temperature and high energy of the MS and its interface ?
4.2.3.32 (+)-copalyl-diphosphate
-
 Picea abies 13-hydroxy-8(14)-abietene + diphosphate the initial products formed and released in vitro are unstable alcohols identified as epimeric thermally unstable allylic tertiary alcohols, 13-hydroxy-8(14)-abietene. The dehydration of the alcohol products, yielding the well established diterpene products levopimaradiene, abietadiene, neoabietadiene, and palustradiene, occurs due to the conditions of the GC-MS analysis typically used ?

Synonyms

EC Number Synonyms Comment Organism
4.2.3.18 PaLAS
-
 Picea abies
4.2.3.32 levopimaradiene/abietadiene synthase
-
 Picea abies
4.2.3.32 PaLAS
-
 Picea abies